Assessing the occurrence and transfer dynamics of ESBL/pAmpC-producing Escherichia coli across the broiler production pyramid

Extended-spectrum \u3b2-lactamase (ESBL)- and plasmid mediated AmpC-type cephalosporinase (pAmpC)-producing Escherichia coli (ESBL/pAmpC E. coli) in food-producing animals is a major public health concern. This study aimed at quantifying ESBL/pAmpC-E. coli occurrence and transfer in Italy's broiler production pyramid. Three production chains of an integrated broiler company were investigated. Cloacal swabs were taken from parent stock chickens and offspring broiler flocks in four fattening farms per chain. Carcasses from sampled broiler flocks were collected at slaughterhouse. Samples were processed on selective media, and E. coli colonies were screened for ESBL/pAmpC production. ESBL/pAmpC genes and E. coli phylogroups were determined by PCR and sequencing. Average pairwise overlap of ESBL/pAmpC E. coli gene and phylogroup occurrences between subsequent production stages was estimated using the proportional similarity index, modelling uncertainty in a Monte Carlo simulation setting. In total, 820 samples were processed, from which 513 ESBL/pAmpC E. coli isolates were obtained. We found a high prevalence (92.5%, 95%CI 72.1-98.3%) in day-old parent stock chicks, in which blaCMY-2 predominated; prevalence then dropped to 20% (12.9-29.6%) at laying phase. In fattening broilers, prevalence was 69.2% (53.6-81.3%) at the start of production, 54.2% (38.9-68.6%) at slaughter time, and 61.3% (48.1-72.9%) in carcasses. Significantly decreasing and increasing trends for respectively blaCMY-2 and blaCTX-M-1 gene occurrences were found across subsequent production stages. ESBL/pAmpC E. coli genetic background appeared complex and bla-gene/phylogroup associations indicated clonal and horizontal transmission. Modelling revealed that the average transfer of ESBL/pAmpC E. coli genes between subsequent production stages was 47.7% (42.3-53.4%). We concluded that ESBL/pAmpC E. coli in the broiler production pyramid is prevalent, with substantial transfer between subsequent production levels

A search for millimetric emission from Gamma Ray Bursts

We have used the 2- year Differential Microwave Radiometer data from the COsmic Background Explorer (COBE) satellite to systematically search for millimetric (31 - 90 GHz) emission from the Gamma Ray Bursts (GRBs) in the Burst And Transient Source Experiment (BATSE) GRB 3B catalog. The large beamsize of the COBE instrument (7 degs FWHM) allows for an efficient search of the large GRB positional error boxes, although it also means that fluxes from (point source) GRB objects will be somewhat diluted. A likelihood analysis has been used to look for a change in the level of millimetric emission from the locations of 81 GRB events during the first two years (1990 & 1991) of the COBE mission. The likelihood analysis determined that we did not find any significant millimetric signal before or after the occurance of the GRB. We find 95% confidence level upper limits of 175, 192 and 645 Jy or, in terms of fluxes, of 9.6, 16.3 and 54.8 10^{-13} erg/cm^2/s, respectively at 31, 53 and 90 GHz. We also look separately at different classes of GRBs, including a study of the top ten (in peak flux) GRBs, the "short burst" and "long burst" subsets, finding similar upper limits. While these limits may be somewhat higher than one would like, we estimate that using this technique with future planned missions could push these limits down to \sim 1 mJy.Comment: 21 pages, 5 figures, to be published in The Astrophysical Journa

Effects of the feeding system on performance and myopathy occurrence in two broiler chicken genotypes

To evaluate whether performance and myopathy occurrence differed according to genotype (Cobb 500 vs. Ross 308) and feeding system (AL: ad libitum vs. ER: early restricted from 13 to 23 d of age, vs. LR: late restricted from 27 to 37 d; restriction rate: 80% of ad libitum), 828 day- old male chicks were assigned to 6 groups (2 x 3 arrangement), housed in 36 pens, and controlled for: individual live weight (weekly) and pen feed intake (daily) until slaughter (48 d); white striping and wooden breast occurrence at slaughter. Individual data were analysed by PROC MIXED of SAS (fixed effects: feeding system, genotype, and interaction; random effect: pen); pen feed intake and myopathy occurrence were analysed by PROC GLM and CATMOD, respectively. The feeding system affected performance: at the end of the first period (1-22 d), ER chickens showed lower weight gain (40.5 g/d vs. 47.8 g/d and 48.0 g/d), feed intake (50.8 g/d vs 61.0 g/d and 60.2 g/d) and weight (903 g vs 1056 g and 1059 g) than AL and LR broilers (P<0.001); at the end of the second period (23-48 d), ER chickens showed higher weight gain (98.3 g/d vs 93.6 g/d and 90.2 g/d) and feed intake (182 g/d vs. 177 g/d and 171 g/d) compared to AL and LR chickens (P<0.001). Final live weight was the highest in AL group, intermediate in ER group, and the lowest in the LR one (3482 g, 3454 g, and 3399 g; P<0.01). Feed conversion in the whole period did not change with the feeding system. At gross examination, white striping occurrence changed from 77.8% to 67.1%, and 81.7% in AL, ER and LR broilers (p<0.10). Differences between genotypes were evident from the first day and, at the end of the trial, weight gain (74.3 g/d vs 70.1 g/d), feed intake (126 g/d vs 114 g/d), feed conversion (1.69 vs 1.64), and live weight (3548 g vs. 3342 g) were higher in the Ross than in the Cobb chickens (P<0.001). At slaughter, the rate of white-striped breasts was similar (on average 75.5%), but the occurrence of severely white-striped breasts was higher in the Ross than in the Cobb chickens (25.9% vs. 7.41%; P<0.001). Wooden breast occurrence (on average 5.1%) did not change with the feeding system or the genotype. In conclusion, under our conditions, a late feed restriction did not permit to recover performance at the end of the trial nor to control white striping occurrence. Moreover, the genotype affected growth rate and white striping degree: the highest the growth rate, the highest the severity of white striping

COBE Observations of the Microwave Counterparts of Gamma Ray Bursts

We have used the data from the COBE satellite to search for delayed microwave emission (31 - 90 GHz) from Gamma Ray Bursts (GRBs). The large $7^\circ$ beam of COBE is well matched to the large positional uncertainties in the GRB locations, although it also means that fluxes from (point source) GRB objects will be diluted. In view of this we are doing a statistical search of the GRBs which occurred during the currently released COBE DMR data (years 1990 and 1991), which overlap $\sim 200$ GRBs recorded by GRO. Here we concentrate on just the top 10 GRBs (in peak counts/second). We obtain the limits on the emission by comparing the COBE fluxes before and after the GRB at the GRB location. Since it is thought that the microwave emission should lag the GRB event, we have searched the GRB position for emission in the few months following the GRB occurrence.Comment: 5 pages, LaTE

A Radio Determination of the Time of the New Moon

The detection of the New Moon at sunset is of importance to communities based on the lunar calendar. This is traditionally undertaken with visual observations. We propose a radio method which allows a higher visibility of the Moon relative to the Sun and consequently gives us the ability to detect the Moon much closer to the Sun than is the case of visual observation. We first compare the relative brightness of the Moon and Sun over a range of possible frequencies and find the range 5--100\,GHz to be suitable. The next consideration is the atmospheric absorption/emission due to water vapour and oxygen as a function of frequency. This is particularly important since the relevant observations are near the horizon. We show that a frequency of $\sim 10$ GHz is optimal for this programme. We have designed and constructed a telescope with a FWHM resolution of 0$^\circ{}\!\!$.6 and low sidelobes to demonstrate the potential of this approach. At the time of the 21 May 2012 New Moon the Sun/Moon brightness temperature ratio was $72.7 \pm 2.2$ in agreement with predictions from the literature when combined with the observed sunspot numbers for the day. The Moon would have been readily detectable at $\sim 2^{\circ}$ from the Sun. Our observations at 16\,hr\,36\,min UT indicated that the Moon would have been at closest approach to the Sun 16\,hr\,25\,min earlier; this was the annular solar eclipse of 00\,hr\,00\,min\,UT on 21 May 2012.Comment: 11 pages, 15 figures, accepted for publication in MNRA

Guidelines for the LTO Noise Assessment of Future Civil Supersonic Aircraft in Conceptual Design

One of the most critical regulatory issues related to supersonic flight arises from limitations imposed by community noise acceptability. The most efficient way to ensure that future supersonic aircraft will meet low-noise requirements is the verification of noise emissions from the early stages of the design process. Therefore, this paper suggests guidelines for the Landing and Take-Off (LTO) noise assessment of future civil supersonic aircraft in conceptual design. The supersonic aircraft noise model is based on the semi-empirical equations employed in the early versions of the Aircraft NOise Prediction Program (ANOPP) developed by NASA, whereas sound attenuation due to atmospheric absorption has been considered in accordance with SAE ARP 866 B. The simulation of the trajectory leads to the prediction of the aircraft noise level on ground in terms of several acoustic metrics (LAmax, SEL, PNLTM and EPNL). Therefore, a dedicated validation has been performed, selecting the only available supersonic aircraft of the Aircraft Noise and Performance database (ANP), that is, the Concorde, through the matching with Noise Power Distance (NPD) curves for LAmax and SEL, obtaining a maximum prediction error of +/- 2.19%. At least, an application to departure and approach procedures is reported to verify the first noise estimations with current noise requirements defined by ICAO at the three certification measurement points (sideline, flyover, approach) and to draw preliminary considerations for future low-noise supersonic aircraft design

COMPASS: a 2.6m telescope for CMBR polarization studies

COMPASS (COsmic Microwave Polarization at Small Scale) is an experiment devoted to measuring the polarization of the CMBR. Its design and characteristics are presented

Detection of pathological lesions in slaughtered rabbits

The slaughterhouse is considered an important control point for the monitoring of rabbit diseases. In our study, 59,440 rabbit carcasses were examined, but only 1% of pathological lesions were recorded at postmortem inspection. Mainly affected were tegumentary, digestive and urinary systems. The most consistent lesion was the subcutaneous abscess; nephritis, probably caused by Encephalitozoon cuniculi, was also frequent. Pathological alterations of the liver, classified as "necrotizing hepatitis" and localized at the caudate lobe, were observed for the first time