Potential Application of Yeast β-Glucans in Food Industry

Different β-glucans are found in a variety of natural sources such as bacteria, yeast, algae, mushrooms, barley and oat. They have potential use in medicine and pharmacy, food, cosmetic and chemical industries, in veterinary medicine and feed production. The use of different β-glucans in food industry and their main characteristics important for food production are described in this paper. This review focuses on beneficial properties and application of β-glucans isolated from different yeasts, especially those that are considered as waste from brewing industry. Spent brewer’s yeast, a by-product of beer production, could be used as a raw-material for isolation of β-glucan. In spite of the fact that large quantities of brewer’s yeast are used as a feedstuff , certain quantities are still treated as a liquid waste. β-Glucan is one of the compounds that can achieve a greater commercial value than the brewer’s yeast itself and maximize the total profitability of the brewing process. β-Glucan isolated from spent brewer’s yeast possesses properties that are benefi cial for food production. Therefore, the use of spent brewer’s yeast for isolation of β-glucan intended for food industry would represent a payable technological and economical choice for breweries

Chemical Constituents of Fruit Wines as Descriptors of their Nutritional, Sensorial and Health-Related Properties

Functional foods are foods that provide positive health effects apart from the provision of essential nutrients. Along with nutraceuticals, they represent the top trends in the food industry. Fruit wines are considered functional foods. When assessing the fruit wine quality, a wide range of descriptors are taken into consideration, namely physicochemical and sensorial properties of fruit wine. Furthermore, within the context of the new food products development (e.g. functional products), functional properties of fruit wines are also taken into consideration. Functional properties are determined by the content of the biologically active components, such as polyphenols, vitamins and micro- and macrominerals. It is also important to consider the food-safety issues regarding the fruit wines consummation, that is, the presence of pesticides, mycotoxins and biogenic amines in different fruit wines. This chapter aims to give an overview of various factors used to evaluate the quality and the functional properties of fruit wines

ESEM Comparative Studies of Hop (Humulus lupulus L.) Peltate and Bulbous Glandular Trichomes Structure

Hop glandular trichomes are developing on the epidermis of hop cones bracts. The anticline cross cut of hop peltate glandular trichomes in Environment Scanning Electron Microscopy (ESEM) observations confirmed spheroid shape of cells. In our research, length of internal peltate glandular trichomes cells varied from 16.00 to 21.47 μm, width from 4.21 to 4.70 μm and volume from 1.19 × 10-6 to 1.99 × 10-6 mm3. The diameter of cell walls varied from 2.11 to 2.94 μm. Volume of observed peltate glandular trichomes varied from 1.65 × 10-2 to 1.95 × 10-2 mm3. The cell structure of bulbous glandular trichomes in anticline cross cut was not observed. According to 121 observations, bulbs formation was visible on the surface of peltate glandular trichomes in the last phase of morphogenesis. Biosynthesis of hop secondary metabolites and activation of their genetic mechanisms are the most intensive at the end of technological maturity of hop cones of hop cultivar Aurora. It is possible that bulbous glandular trichomes represent the final stage of peltate glandular trichome morphogenesis, instead of being separate morphological formations. During biosynthesis of hops secondary metabolites partial pressures of liquids and gasses increase causing breakage of cell walls, which withdraw towards tunica and form papillary texture

Karakterizacija β-glukana izoliranih iz pivskoga kvasca te osušenih različitim metodama

Two different procedures have been used for isolation of water-insoluble ß-glucans from brewer’s yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained ß-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. ß-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of ß-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1→3)-ß-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in ß-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.Netopljivi β-glukani izolirani su iz pivskoga kvasca primjenom dvaju različitih postupaka: alkalno-kiselinskog (AK) i alkalno-kiselinskog s uklanjanjem manoproteina (AKM). Dobiveni pripravci β-glukana osušeni su na zraku, liofilizacijom i raspršivanjem uz prethodnu obradu ultrazvukom. Takvi su pripravci, dobiveni AK i AKM postupcima, imali približno iste vrijednosti suhe tvari, ukupnih polisaharida i proteina, te udjele ugljika, dušika i kisika određene organskom mikroanalizom. Na udio β-glukana u ukupnim polisaharidima bitno su utjecali različiti postupci izolacije. FT-IR spektri svih pripravaka imali su izgled tipičan za (1→3)-β-D-glukan. Sušenjem na zraku i liofilizacijom došlo je do značajnih promjena strukture čestica i aglomeracije ß-glukana. Sušenje raspršivanjem uz prethodnu obradu ultrazvukom nije dovelo do stvaranja nakupina čestica kao ni do znatnih promjena njihove strukture

Potential of bacteria for bioethanol production from lignocellulosic raw materials

Poljoprivreda, šumarstvo i prehrambena industrija izvori su velike količine lignocelulozne biomase, koja može poslužiti kao lako dostupna i jeftina obnovljiva sirovina za dobivanje različitih bioproizvoda. Jedan od takvih proizvoda je i bioetanol. Ovaj rad daje pregled bakterija koje se koriste i/ili istražuju za proizvodnju bioetanola iz lignoceluloznih sirovina. U navedenim istraživanjima proizvodnje bioetanola pomoću bakterija primijenjuju se različiti pristupi kako bi se povećala ekološka i ekonomska efikasnost procesa. Pored uobičajenih bioprocesa, koji se provode u više faza i uz pomoć monokulture, razvijaju se i visokointegrirani (konsolidirani) bioprocesi uz primjenu mikrobnih kokultura.Agriculture, forestry and food industry are sources of large quantities of lignocellulosic biomass, which can be used as an easily accessible and cheap renewable raw material for production of different bioproducts. One of these bioproducts is also bioethanol. This paper provides an overview of bacteria used and/or investigated for bioethanol production from lignocellulose-containing feedstocks. In the research of bioethanol production using bacteria, various approaches are applied in order to increase ecological and economic efficiency of bioprocess. In addition to conventional multi-stage bioprocesses that are carried out using monoculture, highly integrated (consolidated) bioprocesses and applications of microbial cocultures are also in developing stage

Yeasts for bioethanol production from lignocellulose hydrolyzates

Velika količina lignoceluloznog otpada, kojeg čine uglavnom celuloza, hemiceluloza i lignin, nastaje u šumarstvu, poljoprivredi i prehrambenoj industriji. Obzirom na dostupne količine ovih jeftinih i obnovljivih sirovina u Republici Hrvatskoj, važno je razmotriti mogućnosti njihove primjene u proizvodnji bioetanola i raznih drugih biotehnoloških proizvoda. Lignocelulozni hidrolizati su složene smjese heksoza i pentoza te drugih spojeva od kojih neki mogu djelovati kao inhibitori fermentacije. U ovom radu dan je pregled kvasaca koji se koriste i/ili istražuju za proizvodnju etanola iz lignoceluloznih hidrolizata.Huge amounts of lignocellulosic waste, consisting mainly of cellulose, hemicellulose and lignin, are ge- nerated through forestry, agriculture and food industry. Given the available amount of these cheap and renewable raw materials in the Republic of Croatia, it is important to consider the possibility of their conversion into ethanol and other biotechnological products. Lignocellulosic hydrolysates are complex mixtures of hexoses, pentoses and other compounds, some of which may act as fermentation inhibitors. This paper reviews yeast strains that are used and/or studied for ethanol production by fermentation of lignocellulosic hydrolyzates

REINVENTING THE TRADITIONAL PRODUCTS - THE CASE OF BLACKBERRY WINE

Blackberry wine is traditionally produced in the continental parts of Croatia and consumed in moderate quantities as a dessert wine. It is often used as a popular remedy for anaemia and iron deficiency, as well as for alleviating sleep disorders, because of its mineral composition comprising among other elements, iron and magnesium. Besides minerals, blackberry wine is a good natural source of strong antioxidants, such as phenolic acids, anthocyanins, flavonols, catechins and other flavonoids. For a long time, fruit wines in Croatia were mainly produced by traditional methods in small scale. Therefore, the wines were of inconsistent characteristics and often lower quality. Small and medium scale producers are now implementing the more appropriate modern production technologies to achieve good fermentation control and produce high-quality blackberry wine. Consumers are looking for a fruit wine with traditional, geographically unique characteristics, as well as appropriate enological properties, sensory attributes and high added value (e.g. wine rich in bioactive compounds). This is in accordance with the trends in the food industry and food markets (functional, organic) that are geared towards the development of new products with the high added value associated with health or well-being of environment and society. This work aims at reinventing the traditional product blackberry wine, through the prism of claims and requirements for functional and organic foods

Karakterizacija β-glukana izoliranih iz pivskoga kvasca te osušenih različitim metodama

Two different procedures have been used for isolation of water-insoluble ß-glucans from brewer’s yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained ß-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. ß-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of ß-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infrared (FTIR) spectra of all preparations had the appearance typical for (1→3)-ß-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in ß-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.Netopljivi β-glukani izolirani su iz pivskoga kvasca primjenom dvaju različitih postupaka: alkalno-kiselinskog (AK) i alkalno-kiselinskog s uklanjanjem manoproteina (AKM). Dobiveni pripravci β-glukana osušeni su na zraku, liofilizacijom i raspršivanjem uz prethodnu obradu ultrazvukom. Takvi su pripravci, dobiveni AK i AKM postupcima, imali približno iste vrijednosti suhe tvari, ukupnih polisaharida i proteina, te udjele ugljika, dušika i kisika određene organskom mikroanalizom. Na udio β-glukana u ukupnim polisaharidima bitno su utjecali različiti postupci izolacije. FT-IR spektri svih pripravaka imali su izgled tipičan za (1→3)-β-D-glukan. Sušenjem na zraku i liofilizacijom došlo je do značajnih promjena strukture čestica i aglomeracije ß-glukana. Sušenje raspršivanjem uz prethodnu obradu ultrazvukom nije dovelo do stvaranja nakupina čestica kao ni do znatnih promjena njihove strukture