Massive Star Formation at High Angular Resolution : Pyramid Wavefront Sensing and the Search for Massive Binaries

Anders als bei Sternen niedriger Massen ist die Entstehung von Sternen mit Massen oberhalb von 10 Sonnenmassen noch nicht geklaert. Die Gruende sind, dass diese Sterne tief in Staub eigebettet, im allgemeinen weit entfernt (> 1kpc) und aeusserst selten sind. Eine Moeglichkeit die Entstehung massereicher Sterne zu entschluesseln, liegt darin, die Parameter massereicher Doppel- und Mehrfachsysteme zu untersuchen, da diese Parameter Informationen ueber den Entstehungsmechanismus enthalten. Um dieses Projekt durchzufuehren, braucht man Aufnahmen hoher Winkelaufloesung wie sie z.B. mit Hilfe adaptiver Optik erzielt werden koennen. Zu deren Verbesserung wurde der im Infraroten arbeitende Pyramiden Wellenfront Sensor PYRAMIR entwickelt. Es konnte gezeigt werden, dass dieser Sensor eine Verbesserung gegenueber dem konventionellen Shack-Hartmann Sensor darstellt. Das Instrument konnte leider wegen technischer Schwierigkeiten nicht fuer unsere Zwecke eingesetzt werden. Stattdessen wurden mittels Lucky Imaging 150 Targets in den Cep OB2 und OB3 Assoziationen beobachtet. Das aus den Daten abgeleitete Entstehungsszenario zeigt einen urspruenglich weiten, massearmen Doppelstern der durch Akkretion zu einem engen massereicheren heranwaechst. Es ergeben sich grosse Unterschiede zwischen Sternen leichter und schwerer als 10 sonnenmassen in Zahl und Masse der Begleiter. Dieser Befund bestaetigt Resultate aus anderen Sternentstehungsgebieten

Scaleable microscale perfusion systems for yeast and mammalian cells to accelerate process development of bioproducts

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Inbred lab mice are not isogenic:Genetic variation within inbred strains used to infer the mutation rate per nucleotide site

For over a century, inbred mice have been used in many areas of genetics research to gain insight into the genetic variation underlying traits of interest. The generalizability of any genetic research study in inbred mice is dependent upon all individual mice being genetically identical, which in turn is dependent on the breeding designs of companies that supply inbred mice to researchers. Here, we compare whole-genome sequences from individuals of four commonly used inbred strains that were procured from either the colony nucleus or from a production colony (which can be as many as ten generations removed from the nucleus) of a large commercial breeder, in order to investigate the extent and nature of genetic variation within and between individuals. We found that individuals within strains are not isogenic, and there are differences in the levels of genetic variation that are explained by differences in the genetic distance from the colony nucleus. In addition, we employ a novel approach to mutation rate estimation based on the observed genetic variation and the expected site frequency spectrum at equilibrium, given a fully inbred breeding design. We find that it provides a reasonable per nucleotide mutation rate estimate when mice come from the colony nucleus (~7.9 × 10−9 in C3H/HeN), but substantially inflated estimates when mice come from production colonies

The Milky Way like galaxy NGC 6384 and its nuclear star cluster at high NIR spatial resolution using LBT/ARGOS commissioning data

We analyse high spatial resolution near infra-red (NIR) imaging of NGC6384, a Milky Way like galaxy, using ARGOS commissioning data at the Large Binocular Telescope (LBT). ARGOS provides a stable PSF$_{\rm FWHM}\!=\!0.2"\!-\!0.3"$ AO correction of the ground layer across the LUCI2 NIR camera $4'\!\times4'$ field by using six laser guide stars (three per telescope) and a natural guide star for tip-tilt sensing and guiding. Enabled by this high spatial resolution we analyse the structure of the nuclear star cluster (NSC) and the central kiloparsec of NGC6384. We find via 2D modelling that the NSC ($r_{\rm eff}\!\simeq\!10$pc) is surrounded by a small ($r_{\rm eff}\!\simeq\!100$pc) and a larger Sersi\'c ($r_{\rm eff}\!\simeq\!400$pc), all embedded within the NGC\,6384 large-scale boxy/X-shaped bulge and disk. This proof-of-concept study shows that with the high spatial resolution achieved by ground-layer AO we can push such analysis to distances previously only accessible from space. SED-fitting to the NIR and optical HST photometry allowed to leverage the age-metallicity-extinction degeneracies and derive the effective NSC properties of an young to old population mass ratio of $8\%$ with ${\cal M}_{\rm\star,old}\!\simeq\!3.5\times10^7M_\odot$, Age$_{\rm old,\ young}\!=\!10.9\pm1.3$Gyr and 226\,Myr$\pm62\%$, metallicity [M/H]$=\!-0.11\pm0.16$and$0.33\pm39\%$dex, and$E(B\!-\!V)\!=\!0.63$ and 1.44mag.Comment: 12 pages (+9 appendix), 11 figures, Accepted in MNRA

The effect of continuous liver normothermic machine perfusion on the severity of histological bile duct injury

Static Cold Storage (SCS) injures the bile duct, while the effect of Normothermic Machine Perfusion (NMP) is unknown. In a sub-study of the COPE trial on liver NMP, we investigated the impact of preservation type on histological bile duct injury score (BDIS). Transplants with at least one bile duct biopsy, either at end of preservation or 1 h post-reperfusion, were considered. BDIS was determined by assessing peribiliary glands injury, stromal and mural loss, haemorrhage, and thrombosis. A bivariate linear model compared BDIS (estimate, CI) between groups. Sixty-five transplants and 85 biopsies were analysed. Twenty-three grafts were preserved with SCS and 42 with NMP, with comparable baseline characteristics except for a shorter cold ischemic time in NMP. The BDIS increased over time regardless of preservation type (p = 0.04). The BDIS estimate was higher in NMP [8.02 (7.40–8.65)] than in SCS [5.39 (4.52–6.26), p < 0.0001] regardless of time. One patient in each group developed ischemic cholangiopathy, with a BDIS of 6 for the NMP-preserved liver. In six other NMP grafts, BDIS ranged 7–12 without development of ischemic cholangiopathy. In conclusion, BDIS increases over time, and the higher BDIS in NMP did not increase ischemic cholangiopathy. Thus, BDIS may overestimate this risk after liver NMP

Porcine Liver Normothermic Machine Perfusion: Methodological Framework and Potential Pitfalls

peer reviewedPorcine models of liver normothermic machine perfusion (NMP) are increasingly used in transplant research, although known to be challenging because of their complex methodology and their scarcely documented operational aspects. Here, we aimed to provide a methodological framework for researchers looking to adopt NMP technology in research setting by giving an in-detail account of the implementation of a previously validated porcine liver NMP model. We subjected groups of 3-5 porcine livers to 24 h NMP and, using a trial-and-error principle, introduced stepwise changes in the NMP setting with the objective to obtain stable preservation of liver function and histology for 24 h. Female porcine livers were procured, and packed red-blood-cell perfusate was prepared. Perfusate oxygenation, hemodynamics, markers of hepatic injury (aspartate transaminase [AST]), function (lactate, perfusate pH, bile production), and histology were analyzed. Intermediate analysis was performed within groups and a minimum of 3 (out of 5) failed experiments prompted methodological reevaluation. Overall, 13 liver NMP experiments were needed in 3 phases. In phase 1, loss of oxygenator performance occurred from 6 h onward in 3 consecutive experiments because of perfusate leakage. In phase 2, a plasma-tight hollow fiber oxygenator ensured adequate perfusate oxygenation in 5 experiments. However, portal vein resistance increased during all liver NMP, associated with high perfusate AST levels (range, 106-322 IU/L/100 g) and pan-lobular sinusoidal dilation and hemorrhage, suggesting liver outflow impairment. In phase 3, an improved inferior vena cava cannulation technique avoided liver outflow impairment, resulting in lower AST release (range, 29-101 IU/L/100 g), improved lactate clearance, preserved biliary excretion, and normal histology in 5 experiments. This study underscores the critical importance of auditing all equipment and operational components of NMP circuits to obtain successful and reproducible perfusion setup and advocates for in-detail reporting of methodological aspects and potential pitfalls. © 2021 Wolters Kluwer Health. All rights reserved

A Revised Design for Microarray Experiments to Account for Experimental Noise and Uncertainty of Probe Response

Background Although microarrays are analysis tools in biomedical research, they are known to yield noisy output that usually requires experimental confirmation. To tackle this problem, many studies have developed rules for optimizing probe design and devised complex statistical tools to analyze the output. However, less emphasis has been placed on systematically identifying the noise component as part of the experimental procedure. One source of noise is the variance in probe binding, which can be assessed by replicating array probes. The second source is poor probe performance, which can be assessed by calibrating the array based on a dilution series of target molecules. Using model experiments for copy number variation and gene expression measurements, we investigate here a revised design for microarray experiments that addresses both of these sources of variance. Results Two custom arrays were used to evaluate the revised design: one based on 25 mer probes from an Affymetrix design and the other based on 60 mer probes from an Agilent design. To assess experimental variance in probe binding, all probes were replicated ten times. To assess probe performance, the probes were calibrated using a dilution series of target molecules and the signal response was fitted to an adsorption model. We found that significant variance of the signal could be controlled by averaging across probes and removing probes that are nonresponsive or poorly responsive in the calibration experiment. Taking this into account, one can obtain a more reliable signal with the added option of obtaining absolute rather than relative measurements. Conclusion The assessment of technical variance within the experiments, combined with the calibration of probes allows to remove poorly responding probes and yields more reliable signals for the remaining ones. Once an array is properly calibrated, absolute quantification of signals becomes straight forward, alleviating the need for normalization and reference hybridizations