Evaluation of focal depth

Evaluation of focal depth using 1 μm fluorescent particles suspended in the experimental<br>channel under the microscope, obtained from 400 number of steps. Estimated focal depth about<br>10 μm. <br

S5 Appendix

1. Selected symbols characterizing experiment and materials<br> 2. Non-dimensional parameters characterizing filaments and flow<br> <br

Matlab scripts for diffusion analysis

<p>The Matlab code for the calculation of diffusion coefficients, generation of figures and image processing of ruptured filaments due to thresholding.</p> <p> </p> <p>_exe ended files are executive files, other are functions</p> <p>CentrOrientGabson generates position table and angle table of the fibers</p> <p>figureANGLE and figureMSD generates tif images</p> <p>Gapson connects gaps in a fiber when threshold functon cuts fiber into pieces</p> <p>MSDandDiff calculates Mean Squared Displacement and diffusion coefficients</p> <p> </p> <p>For more information please visit: http://fluid.ippt.pan.pl/nanofibres/ and Hydrogel nanofilaments via core-shell electrospinning by Pawel Nakielski</p

S3 Microchannel preparation

The file include all steps necessary to prepare microchannel.<br><br

Thermal fluctuations and calculation of persistance length - part 1

<p>Data presents thermal fluctuations of nanofilaments for different composition:</p> <p>EA1 - polyacrylamide hydrogel monomer mass ratio 37.5:1 AAm:BIS-AAm</p> <p>EA2 - polyacrylamide hydrogel monomer mass ratio 20:1 AAm:BIS-AAm</p> <p>EA3 - polyacrylamide hydrogel monomer mass ratio 4:1 AAm:BIS-AAm</p> <p>EN1 - polyacrylamide hydrogel monomer mass ratio 37.5:1 NIPAAm:BIS-AAm</p> <p>EN2 - polyacrylamide hydrogel monomer mass ratio 20:1 NIPAAm:BIS-AAm</p> <p>EN3 - polyacrylamide hydrogel monomer mass ratio 4:1 NIPAAm:BIS-AAm</p> <p>For more information please visit: http://fluid.ippt.pan.pl/nanofibres/ and Hydrogel nanofilaments via core-shell electrospinning by Pawel Nakielski</p> <p> </p

S1 Filament knots formation

Video presenting long filament and knots formation during Brownian motion.<br

AFM images of hydrogel nanofilaments

<p>Figures presenting AFM topography of the hydrogel nanofilaments for three different compositions.</p> <p>EA1 - polyacrylamide hydrogel monomer mass ratio 37.5:1 AAm:BIS-AAm</p> <p>EA2 - polyacrylamide hydrogel monomer mass ratio 20:1 AAm:BIS-AAm</p> <p>EA3 - polyacrylamide hydrogel monomer mass ratio 4:1 AAm:BIS-AAm</p> <p> </p> <p>EN1 - polyacrylamide hydrogel monomer mass ratio 37.5:1 NIPAAm:BIS-AAm</p> <p>EN2 - polyacrylamide hydrogel monomer mass ratio 20:1 NIPAAm:BIS-AAm</p> <p>EN3 - polyacrylamide hydrogel monomer mass ratio 4:1 NIPAAm:BIS-AAm</p> <p> </p> <p>For more information please visit: http://fluid.ippt.pan.pl/nanofibres/ and Hydrogel nanofilaments via core-shell electrospinning by Pawel Nakielski</p

S4 Pulsate pump

Design of pulsate pump by K.Z.<br

Nanofibers shell dissolution

<p>Movie presents dissolution of nanofibers polymer shell. Highly flexible nanofilaments can be observed.</p> <p> </p> <p>For more information please visit: http://fluid.ippt.pan.pl/nanofibres/ and Hydrogel nanofilaments via core-shell electrospinning by Pawel Nakielski</p

Description of filament orientation evaluation

Evaluating orientation, elongation and bending characteristic (definitions)<br> <br