Synthesis of 3D porous CeO2/reduced graphene oxide xerogel composite and low level detection of H2O2

A novel synthetic approach has been designed to prepare CeO2/reduced graphene oxide (rGO) xerogel composite. The CeO2/rGO xerogel composite electrode displays much enhanced performance for the catalytic reduction of H2O2 than the single component CeO2. The CeO2/rGO modified glassy carbon electrode displayed a wide linear range (60.7 nM – 3.0 μM), and low level of detection limit (30.40 nM) for H2O2 and much higher sensitivity than that of CeO2 nanoparticles modified electrode. The sensor fabricated by the xerogel composite was fast, stable, and reliable to the detection of hydrogen peroxid

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Not AvailableKlebsiella pneumoniae is an important emerging pathogen of humans and animals which may lead to major clinical implications including mortality. Moreover the increased use of antibiotics has promoted emergence of carbapenem resistant strains and extended spectrum β-lactamase producers (ESBLs) of K. pneumoniae. Recently, phage therapy has gained momentum as a conceivable alternative against emerging antibiotic resistance. OBJECTIVE: With the aim to explore the efficacy of phage therapy against virulent K. pneumoniae infection, the current study was undertaken to assess the therapeutic effects of a novel lytic phage-VTCCBPA43 in pneumonic mouse model. METHODS: The tailed phage - VTCCBPA43 was assessed for it's growth kinetics, in vitro host range analysis, temperature and pH sensitivity. The protein constituents were analysed by SDS-PAGE and Lc MS/MS and the therapeutic efficacy was observed 2 hr post challenge with virulent K. pneumoniae in BALB/c mouse model. RESULTS: The phage-VTCCBPA43 was found to exhibit high temperature (upto 80 °C) tolerant property. It was most active at pH 5, had a burst size of 172 PFU/ml and exhibited a narrow host range. It was identified as KP36 like phage by shotgun proteomics. Following intranasal application of a single dose of 2 × 109 PFU/mouse post challenge, presence of biologically active phage in vivo and a significant reduction of bacterial load in lungs at all time points was observed which was even more impressive at 96hpi, 6dpi and 10dpi. The loss of severity of lesions suggested overall beneficial effects of phage therapy using BPA43 in the pneumonic mouse model. CONCLUSION: The current research represents first in vivo evidences for effective phage therapy against K. pneumoniae infection by using intranasal route.Not Availabl

Development of nano-spherical RuO2 active material on AISI 317 steel substrate via pulse electrodeposition for supercapacitors

The goal of the present study is to develop a thin film hydrous ruthenium oxide (RuO2) electrode material on Ni flashed AISI 317 stainless steel (SS) substrate by pulse electrodeposition (PED) technique for application in supercapacitors. The nickel (Ni) strike thin film is deposited prior to RuO2 in order to improve the adhesion of the active material on the SS substrate. The prepared RuO2 active material on Ni strike SS electrode is characterized using XRD, SEM with EDAX and electrochemically using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using an IVIUM reference potentiostat. The surface morphologies of the thin film (thickness of 2.3 μm) active materials are nano-spherical shaped and the particles are arranged uniformly without cracks on the SS substrate. The electrochemical impedance and CV profile demonstrates its superior characteristics in the electrochemical system. Moreover, the specific capacitance of RuO2 value is approximately 520 F g− 1 at the scan rate of 1 mV s− 1 and it's indicating a better utilization of active species in supercapacitors