Cross-correlation functions calculated for large gull and tern detrended and standardised breeding population abundance (number of breeding pairs) from 1975–2013 (excluding 2003) for a) large gull and Arctic Terns, b) large gull and Common Terns, c) large gulls and Sandwich Terns and d) large gulls and Roseate Terns.

<p>A significant negative correlation (where the correlation coefficient lay outside the lower 2.5% confidence interval) was found at lag 0 for Roseate Terns (r_t = -0.40). There was a significant positive correlation between large gull and Common Tern abundance at lag 0 (r_t = 0.51). There were no significant correlations between large gull and Sandwich Tern and large gull and Arctic Tern breeding population abundances at any time lag.</p

Cross-correlation functions calculated for large gull detrended and standardised breeding population abundance (number of breeding pairs) and tern productivity data from 1991–2013 (excluding 2003) for a) large gull and Arctic Terns, b) large gull and Common Terns and c) large gulls and Roseate Terns.

<p>A significant negative correlation (where the correlation coefficient lay outside the lower 2.5% confidence interval) was found at lag 2 for Arctic (r_t+2 = -0.51) and Common Terns (r_t+2 = -0.77). There was no significant correlation between large gull breeding abundance and Roseate Tern productivity at any time lag.</p

Productivity of three tern species on Coquet Island.

<p>Time series showing annual productivity (number of fledged chicks per nest) of Arctic, Common and Roseate Tern populations breeding on Coquet Island from 1991–2013.</p

MOESM9 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 9: Figure S8. The samples of A. fumigatus-SD100, A. fumigatus-SD256 and A. fumigatus-SD257 pellets. 1) A. fumigatus-SD100 (left), A. fumigatus-SD256 after CO2 limitation (right); 2) A. fumigatus-SD100 (left), A. fumigatus-SD257 after CO2 limitation (right)

MOESM4 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 4: Figure S3. Biomass production and lipid yields of A. fumigatus growing on different carbon sources. A. fumigatus/NEC: A. fumigatus grown on FGB with no extra carbon source; A. fumigatus/GLU: A. fumigatus grown on FGB with 20% glucose; A. fumigatus/TWS: A. fumigatus grown on FGB with 1% TWS. Significance levels: *) p<0.05; **) p<0.01

MOESM7 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 7: Figure S6. Environmental scanning electron microscopy of A. fumigatus and A. fumigatus-Synechocystis PCC 6803 associations. (A,B) A. fumigatus pellets growing in liquid media; (C) A. fumigatus-SD100 and (D) A. fumigatus-SD216 pellets

MOESM8 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 8: Figure S7. Increase in biomass and lipid/Intracellular FFA yield in A. fumigatus-Synechocystis pellets after flocculation. (A) Increase in lipids in A. fumigatus/GLU-SD pellets compare to the additive yields of lipid/intracellular FFA and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. (B) Increase in lipids in A. fumigatus/TWS-SD pellets compare to the additive yields of lipid/intracellular FFAs and extracellular FFAs extracted from mono-cultured A. fumigatus and SD strains before co-cultivation. Significance levels: *) p<0.05; **) p<0.01

MOESM1 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 1: Figure S1. Artistic representation of two strategies designed for secretion of FFAs to growth media: ‘FFA Secretion’ and ‘Green Recovery’ from Synechocystis PCC 6803 cells. Text in red represents genetic modifications of SD100 strains

MOESM3 of Lipid production in association of filamentous fungi with genetically modified cyanobacterial cells

Additional file 3: Figure S2. Pelletization of A. fumigatus grown in liquid media. (A) A. fumigatus pellets grown on FGB with no extra carbon source (left); with 1% TWS (middle) and with 20% glucose (right); (B) A. fumigatus pellets grown on FGB with 20% glucose (viewed from bottom); (C) A. fumigatus pellets grown on FGB with 20% glucose (left) and with 1% TWS (right). Scale=5mm