DEVELOPMENT AND VALIDATION OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR GABAPENTIN AND ITS RELATED SUBSTANCES IN CAPSULE DOSAGE FORM AND EXCIPIENT COMPATIBILITY STUDIES

Objective: A simple, accurate, precise, and reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for gabapentin (GBP) and its related substances in the capsule dosage form and excipient compatibility studies. Methods: The review of literature indicates that various methods have been reported for the estimation of GBP. When some excipients were used for GBP, it produced degradation product called lactam due to the presence of more water content. Hence, a novel RP-HPLC method has been developed for studying excipient compatibility and related substances of GBP in capsule dosage form using excipients such as lactose anhydrous and dried maize starch which is having less water activity. Waters Alliance e2695 separation module with ultraviolet/photodiode array (UV/PDA) detector with Inertsil C8 (250 mm×4.6 mm); 5 μm with an injection volume of 50 μl is injected and eluted with the (gradient program) mobile Phase A buffer: acetonitrile (940:60) and mobile phase B buffer: acetonitrile (700:300) pH 6.9 with 5 N potassium hydroxide which is pumped at a speed of 1.5 ml/min and detected by UV/PDA detector at 210 nm. The peaks of GBP and GBP-related compound A are well separated at 6.7 min and 34.5 min, respectively. Results: The method developed was approved for various parameters such as accuracy, specificity, precision, intermediate precision, range, linearity, robustness, limit of detection, limit of quantification, steadiness, and system suitability according to the International Conference on Harmonization guidelines. The results got were according to the acceptance criteria. Conclusion: The technique proposed was assured for detection of related substances in the marketed formulation and could be used for the routine analysis of GBP and GBP-related compound A in the capsule dosage form

VALERIANA JATAMANSI: AN ETHNOBOTANICAL REVIEW

 Objective: The main objective of review is an effort to give detailed information on Valeriana jatamansi and its multiple medicinal properties. Methods: As per the literature, the plant V. jatamansi is a perennial herb and found at subtemperate Himalayan regions of the Kumaon and Garhwal. The plant most popularly known as Valerian.†The plant belongs to the family Valerianaceae. The roots and rhizomes of the plants are traditionally used to treat ulcers, jaundice, dry cough, seminal weakness, skin diseases, leprosy, and for sleep enhancement due to the presence of a variety of different chemical constituents such as sesquiterpenes, coumarins, iridoids, lignanoids, alkaloids, and flavonoids. From literature, the reported methods performed for different pharmacological activities have been thoroughly discussed and complied. Results: Most of the studies compiled from literature shown that V. jatamansi possess antimicrobial, antioxidant, anti-inflammatory, cytotoxic, anxiolytic, and neuroprotective activities. Conclusion: The review reveals that continuous research on this plant is needed to know the exact molecular mechanism and further elaborative studies can lead to develop safe therapeutic use in modern medicine

Method development and validation for the simultaneous estimation of Atazanavir and Ritonavir in tablet dosage form by RP-HPLC

The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Atazanavir and Ritonavir in pure and pharmaceutical dosage forms of Atazanavir and Ritonavir were well separated using a X-Tera C18 (100 x 4.6mm, 3.5�m) and Mobile phase consisting of Buffer(pH-2.5): Acetonitrile (40:60) adjusted to pH- 2.5 at the flow rate 1.2 ml/min and the detection was carried out at 247nm with PDA detector. The Retention time for Atazanavir and Ritonavir were found to be 1.982 & 2.576 respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Metronidazole and Norfloxacin combination pharmaceutical dosage form