1 research outputs found
Successful production of recombinant buckwheat cysteine-rich aspartic protease in Escherichia coli
U ovom radu predstavljena je ekspresija rekombinantne atipiÄne aspartatne proteinaze heljde (Fagopyrum esculentum) bogate cisteinom, gde su testirana razliÄita ekspresiona svojstva pet sojeva E. coli. TakoÄe je analiziran i uticaj fuzionih partnera (His6 i MBP) na efikasnost ekspresije. U sluÄaju His6-FeAPL1, dobijena je velika koliÄina nerastvornog proteina, smeÅ”tenog u inkluzionim telima. S druge strane, MBP-FeAPL1 je bio lokalizovan i u citoplazmi i u inkluzionim telima u oba upotrebljena soja E. coli (BL21 i Rosetta-gami). MeÄutim, samo za rekombinantni protein proizveden u soju Rosetta-gami, dokazana je proteolitiÄka aktivnost na supstratu BSA, pri pH 3,0. Rezultati su takoÄe ukazali da FeAPL1 sadrži PRO segment, Äije je odstranjivanje neophodno za njegovu proteolitiÄku aktivnost. Aktivnost FeAPL1, pokazana samo u soju Rosetta-gami, gde je moguÄe formiranje disulfidnih veza, ukazuje na znaÄaj 12 cisteina u uspostavljanju pravilne strukture koja omoguÄava funkcionalnost enzima.Herein, the expression of recombinant cysteine-rich atypical buckwheat (Fagopyrum esculentum) aspartic protease (FeAPL1) in five Escherichia coli strains differing in their expression capabilities is presented. It was shown that the expression success depended highly on the choice of FeAPL1 fusion partner. His6-FeAPL1 was produced in large quantities as an insoluble protein localized in inclusion bodies. On the other hand, MBP-FeAPL1 was localized in both the cytoplasm and inclusion bodies in BL21 and Rosetta-gami strains. Only purified soluble MBP-FeAPL1 from Rosetta-gami cells showed proteolytic activity at pH 3.0 with BSA as the substrate. The results also indicated that FeAPL1 contained a PRO segment that had to be removed for the enzyme activity to appear. The activity of FeAPL1 produced in the Rosetta-gami strain, which enables disulfide bond formation, indicated the importance of the twelve cysteine residues for correct folding and functionality