Bacteriological profile and antibiotic sensitivity of pus isolates in patients at Regional Hospital Center of Franceville in Gabon

Background:Bacterial resistance to antibiotics is a public health problem, it is a threat to the future. It compromises the effectiveness of antibiotic treatment and is constantly increasing worldwide. This work has been highlighted by the profile and sensitivity of pyogenic bacteria isolated at Franceville Regional Hospital Center. Methods:Effective antibiotic strategy, suppurations from the wounds of 530 patients were collected and analyzed. Biochemical identification of isolated bacterial strains was performed with Api 10s galleries and verified using Vitek-2 system. The Kirby-Bauer technic was used to perform the antibiogram. Five antibiotics were first tested in a simple way and then combined in pairs. Results:360 samples showed bacterial strains and 170 were sterile cultures. 360 microorganisms were isolated, of which 230 strains came from hospitalization surgery, 110 from minor surgery and 20 from medicine.The bacterial families identified are Enterobacteriaceae, Moraxellaceae, Pseudomonadaceae, Streptococcaceae and Xanthomonadaceae. Enterobacterizceae is the most common family with 64.69% and has revealed 7 genera Citrobacter, Enterobacter, Escherichia, Klebsiella, Serratia, Yersinia and Proteus 65% antibiotic sensitivity of simple antibiotics showed the best sensitivities with Ciprofloxacin and Ofloxacin a resistance rate 31.25%, 100% unlike Amoxicillin and Metronidazole. Ceftazidim was not very active on bacterial strains with a resistance rate of 93.75%. The interactions between antibiotics combined gave antagonistic, synergistic, indifferent and additive effects. The indifferent effect was much more observed (44 cases), in contrast to the additive effect which represented only 23 cases. The antagonic and synergistic effects were reported in 14 cases. Combined Amoxicillin and Metronidazole, Ceftazidim antibiotics showed good activity on bacterial strains, with good synergy and additive effect, or were not combined. Conclusion:The emergence of bacteria is major global problem multi-resistance. The rationalization of the prescription of antibiotics, especially Ofloxacin and Ciprofloxacin, and the optimization of bacteriological prescriptions are desirable in surgical services. However, rigor in dispensing pharmacies and the rational use of these antibiotics are essential to avoid the emergence of resistance phenomena

Structure elucidation of a new lanostane triterpene from Gabonese Ganoderma orbiforme fruiting bodies

International audienceThe structure and complete nuclear magnetic resonance (NMR) assignments of orbifomitellic acid, a novel lanostane triterpene isolated from the fruiting bodies of a Gabonese Ganoderma orbiforme (Polyporaceae), are reported. Within the vast catalogue of lanostanes documented from Ganoderma spp., orbifomitellic acid is the first disclosing a -COOH group at C-4

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Medicinal plants are traditionally used in Gabon to treat several types of illnesses. The study’s purpose was to determine the toxic, antibacterial, and anti-inflammatory effects of Antrocaryon klaineanum Pierre extracts and to characterize their phytochemical compounds. Toxicity was evaluated on frog tadpoles (Phrynobatrachus africanus Hallowell). The microorganism susceptibility test was performed by the diffusion method, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the microdilution technique. Anti-inflammatory activity was tested through protein denaturation and membrane stabilization methods. Chromatography and molecular network techniques were used to characterize chemical compounds. The lethality test showed that the lethal concentration (LC50) increased from 110.03 ± 1.25 to 15.86 ± 2.21 μg/mL after 24 and 96 hours of exposure. In tadpoles exposed to 7.81 μg/mL extract, the first mortalities (12.5%) were observed on the fifth day of exposure. A relative decrease in mature erythrocytes exposed to plant extracts was observed. The antibacterial activity shows that the Ak F2, Ak F3, and Ak F4 fractions (from the water-ethanol crude extract) gave the greatest antibacterial activities compared to the other extracts. The water, water-acetone, and water-ethanol extracts showed good inhibition of denaturation. The haemolysis test shows that the extracts exhibited good anti-inflammatory activities. Phytochemical characterisation revealed four major compounds, including monogallate epicatechin and hydroxy-ergostadian. The molecular network revealed five main clusters. Our study shows that A. klaineanum Pierre could be a promising natural product for the isolation of molecules with potential biological activities