Foodborne Transmission of Deformed Wing Virus to Ants (Myrmica rubra)

Virus host shifts occur frequently, but the whole range of host species and the actual transmission pathways are often poorly understood. Deformed wing virus (DWV), an RNA virus described from honeybees (Apis mellifera), has been shown to have a broad host range. Since ants are often scavenging on dead honeybees, foodborne transmission of these viruses may occur. However, the role of the ant Myrmica rubra as an alternative host is not known and foodborne transmission to ants has not been experimentally addressed yet. Here, we show with a 16-week feeding experiment that foodborne transmission enables DWV type-A and -B to infect M. rubra and that these ants may serve as a virus reservoir. However, the titers of both plus- and minus-sense viral RNA strands decreased over time. Since the ants were fed with highly virus-saturated honeybee pupae, this probably resulted in initial viral peaks, then approaching lower equilibrium titers in infected individuals later. Since DWV infections were also found in untreated field-collected M. rubra colonies, our results support the wide host range of DWV and further suggest foodborne transmission as a so far underestimated spread mechanism

Comparison of Observed and Simulated Drop Size Distributions From Large Eddy Simulations With Bin Microphysics

Two case studies of marine stratocumulus (one nocturnal and drizzling, the other daytime and nonprecipitating) are simulated by the UCLA large-eddy simulation model with bin microphysics for comparison with aircraft in situ observations. A high-bin-resolution variant of the microphysics is implemented for closer comparison with cloud drop size distribution (DSD) observations and a turbulent collision–coalescence kernel to evaluate the role of turbulence on drizzle formation. Simulations agree well with observational constraints, reproducing observed thermodynamic profiles (i.e., liquid water potential temperature and total moisture mixing ratio) as well as liquid water path. Cloud drop number concentration and liquid water content profiles also agree well insofar as the thermodynamic profiles match observations, but there are significant differences in DSD shape among simulations that cause discrepancies in higher-order moments such as sedimentation flux, especially as a function of bin resolution. Counterintuitively, high-bin-resolution simulations produce broader DSDs than standard resolution for both cases. Examination of several metrics of DSD width and percentile drop sizes shows that various discrepancies of model output with respect to the observations can be attributed to specific microphysical processes: condensation spuriously creates DSDs that are too wide as measured by standard deviation, which leads to collisional production of too many large drops. The turbulent kernel has the greatest impact on the low-bin-resolution simulation of the drizzling case, which exhibits greater surface precipitation accumulation and broader DSDs than the control (quiescent kernel) simulations. Turbulence effects on precipitation formation cannot be definitively evaluated using bin microphysics until the artificial condensation broadening issue has been addressed

Zebrafish caudal fin as a model to investigate the role of probiotics in bone regeneration

: Probiotics are live microorganisms that confer several beneficial effects to the host, including enhancement of bone mineralization. However, probiotic action on bone regeneration is not well studied and therefore we analysed various effects of probiotic treatment on the caudal fin regeneration of zebrafish. Morphological analysis revealed an increased regenerated area with shorter and thicker lepidotrichia segments after probiotic treatment. Fourier transform infrared spectroscopy imaging analysis highlighted the distribution of phosphate groups in the regenerated fins and probiotic group showed higher amounts of well-crystallized hydroxyapatite. At the midpoint (5 days post amputation) of regeneration, probiotics were able to modulate various stages of osteoblast differentiation as confirmed by the upregulation of some key marker genes such as runx2b, sp7, col10a1a, spp1 and bglap, besides suppressing osteoclast activity as evidenced from the downregulation of ctsk. Probiotics also caused an enhanced cell cycle by regulating the expression of genes involved in Retinoic acid (rarga, cyp26b1) and Wnt/β-catenin (ctnnb1, ccnd1, axin2, sost) signaling pathways, and also modulated phosphate homeostasis by increasing the entpd5a levels. These findings provide new outlooks for the use of probiotics as a prophylactic treatment in accelerating bone regeneration and improving skeletal health in both aquaculture and biomedical fields

Determination of biological and physicochemical parameters of Artemia franciscana strains in hypersaline environments for aquaculture in the Colombian Caribbean

BACKGROUND: Artemia (Crustacea, Anostraca), also known as brine shrimp, are typical inhabitants of extreme environments. These hypersaline environments vary considerably in their physicochemical composition, and even their climatic conditions and elevation. Several thalassohaline (marine) environments along the Colombian Caribbean coast were surveyed in order to contribute to the knowledge of brine shrimp biotopes in South America by determining some vital biological and physicochemical parameters for Artemia survival. Additionally, cyst quality tests, biometrical and essential fatty acids analysis were performed to evaluate the economic viability of some of these strains for the aquaculture industry. RESULTS: In addition to the three locations (Galerazamba, Manaure, and Pozos Colorados) reported in the literature three decades ago in the Colombian Caribbean, six new locations were registered (Salina Cero, Kangaru, Tayrona, Bahía Hondita, Warrego and Pusheo). All habitats sampled showed that chloride was the prevailing anion, as expected, because of their thalassohaline origin. There were significant differences in cyst diameter grouping strains in the following manner according to this parameter: 1) San Francisco Bay (SFB-Control, USA), 2) Galerazamba and Tayrona, 3) Kangarú, 4) Manaure, and 5) Salina Cero and Pozos Colorados. Chorion thickness values were smaller in Tayrona, followed by Salina Cero, Galerazamba, Manaure, SFB, Kangarú and Pozos Colorados. There were significant differences in naupliar size, grouping strains as follows (smallest to largest): 1) Galerazamba, 2) Manaure, 3) SFB, Kangarú, and Salina Cero, 4) Pozos Colorados, and 5) Tayrona. Overall, cyst quality analysis conducted on samples from Manaure, Galerazamba, and Salina Cero revealed that all sites exhibited a relatively high number of cysts.g(-1). Essential fatty acids (EFA) analysis performed on nauplii from cyst samples from Manaure, Galerazamba, Salina Cero and Tayrona revealed that cysts from all sites exhibited high arachidonic acid:20:4(n-6) (ArA) and eicosapentaenoic acid: 20:5(n-3) (EPA) levels comparable to the control sample (SFB). In contrast, most cysts collected (including SFB) at different locations, and during different months, presented low docosahexaenoic acid: 22:6(n-3) (DHA) levels (Manaure was the only exception with high DHA levels). Some variations in EPA and ArA levels were observed in all sites, contrasting with the much lower DHA levels which remained constant for all locations, except for Manaure which exhibited variable DHA levels. DHA/EPA ratio was overall very low for all sites compared to SFB cysts. All strains had a low DHA/ArA, but a high EPA/ArA ratio, including the control. CONCLUSION: The Colombian A. franciscana habitats analyzed were determined to be thalassohaline, and suitable for A. franciscana development. EFA profiles demonstrated that Tayrona, Galerazamba, Manaure and Salina Cero strains are suitable food for marine fish and crustacean culture because of their high EPA/ArA ratio, but might have to be fortified with DHA rich emulsions depending on the nutritional requirements of the species to be cultured, because of their overall low DHA content. The relatively small nauplii are appropriate for marine larvaeculture. In contrast, the strains from Tayrona, Kangarú, Salina Cero, and Pozos Colorados may be of use but limited to Artemia small biomass production quantities, because of the small surface area of their respective locations; Artemia could be exploited at these locations for local aquaculture applications. In general, cyst quality evaluation for Manaure, Salina Cero and Galerazamba cysts revealed that cysts from these three locations could improve their quality by concentrating efforts on cyst processing techniques. Finally, most locations had great A. franciscana production potential and require different degrees of water quality and/or infrastructure management

Aquaculture production, GHG emission and economic growth in Sub-Sahara Africa

Aquaculture is a major source of protein in Sub-Saharan Africa (SSA), a region experiencing rapid population growth, changing lifestyles and preferences, and increased health awareness. However, the industry is still underdeveloped and is of a subsistence nature. Climate change has impacted aquaculture production (AQUAP) in SSA because of greenhouse gas (GHG) emissions. However, AQUAP activities also results in GHG emissions. In SSA, the causal effect of GHG emissions and AQUAP has not yet been empirically established and quantified. The objective of the study was to determine the relationship between GHG emissions and AQUAP in SSA. The parsimonious vector autoregressive (VAR) model was used in the study, with annual time series data of Gross Domestic Product (GDP), meat production (MP), GHG emissions, and AQUAP from 1970 to 2020. The findings demonstrate that AQUAP in SSA was suppressed until 2006 when it suddenly increased. Western and Central Africa have dominated AQUAP in SSA. GHG emissions were dropping sporadically until 1991 when they began to rise gradually. In both the long and short run, GHG emissions had a negative influence on AQUAP, while AQUAP had an asymmetric impact on GHG emissions. AQUAP impacts GDP positively in both the long and short run, and GHG emissions had an asymmetric impact on GDP. In conclusion, GHG emissions negatively affect AQUAP. In addition, AQUAP reduced GHG emissions in the short run but however increased it in the long run. This indicates the infancy of the sector in SSA, the initial phase of the Environmental Kuznets Curves (EKC). Furthermore, GDP is positively affected by both GHG emissions and AQUAP. This also cements the initial stages of the EKC, with economic development also powered by GHG emissions, with also the positive contribution of AQUAP to economic growth. Overall, the study concludes of initial economic, and aquaculture sectoral development powered by GHG emissions. However, this is also leading to increased emissions. The study recommends upscaling AQUAP in SSA given its infancy, huge economic potential, sustainability and low GHG emission potential but should be grounded on environmentally sustainable practices