Effect of Open Field and Open Shade Conditions on the Growth and Phytochemical Constituents of Amaranthus cruentus

The growth and phytochemical constituents of Amaranthus cruentus plants under open shade and open field conditions were studied. Plants grown under open field had significantly higher plant height, number of leaves, fresh weight, dry weight and leaf area throughout the period of the analysis than those grown under open shade.  Plants grown under open field condition had significantly higher (p = 0.05) amount of total flavonoids and total tannin and total antioxidant capacity.  Nevertheless, the total phenol was significantly higher in plants grown under open shade condition.  Leaf extracts of plants grown under open field condition also had significantly higher DPPH scavenging activity based on the IC50 value (38.31 ?g/ml) than those of plants grown under open shade (52.35 ?g/ml).  The results of this study show that Amaranthus cruentus plants should be grown under open field to obtain better vegetative growth as well as higher phytochemical and antioxidant capabilities. KEY WORDS: Amaranthus cruentus, open field, open shade, growth, antioxidan

A re-evaluation of plastochron index determination in peas - a case for using leaflet length

The plastochron index (PI) is a measure of plant growth reports our findings on PI using the average length of and can be used to determine growth rate, based upon the first pair of leaflets on each node. Early leaflet appearance of successive leaves on the axis of the growth in peas occurs exponentially and the early plant. PI should under ideal growth conditions be a stages of growth of successive pairs of leaflets occur at regular event and should be predictable with a relatively the same relative growth rate. Given that growth of small error of a few hours. PI has been variously leaflets during early development can be measured calculated in peas, and each method reported has had successfully, we propose the use of leaflet growth as a with it a number of problems that do not allow for measure of the plastochron index in peas. Our results reasonable prediction of PI. Internode length varies suggest that plant age is best expressed using the greatly and is dependent upon the variety, which may be plastochron index, which is a measure of the time short- or long-stemmed; thus this parameter is not ideal interval between the initiations of successive events — for determining growth rate or plant age. This paper in the case of peas, of successive pairs of leaflets

Plastochron index - an indicator of plant structure and function a case study using Pisum sativum L

The use of chronological age for example, using days after sowing (DAS), or days after germination (DAG) as a time variable may result in the inherent variability between plants resulting in differences which can be large enough to obscure subtle developmental trends that become evident among plants sown at the same time. An alternative to DAS or DAG is the plastochron index (PI), first used by Erickson and Michelini (1957) as a morphological time scale and numerical index; which to according to the authors suggested and represented a more accurate reflection of the developmental status of a plant. The research presented in this thesis was therefore aimed specifically at utilizing the index in qualitative and quantitative analyses, to confirm its usefulness in analyzing and predicting plant growth and development. Specifically this research focused on investigating various morphological and physiological events that together, hopefully, would serve as a template for the prediction of the growth, development and reactions of Pisum sativum L. to different growth conditions. In Chapter 3, the use of the average length of the first pair of leaflets on each node as a suitable parameter for calculating PI in P. sativum is suggested. The results presented in Chapter 3 suggest that plant age is best expressed using the plastochron index, as this reflects the time interval between the initiations of successive pairs of leaflets. This section of the research has been published as “Ade-Ademilua OE, Botha CEJ (2005) A re-evaluation of plastochron index in peas - a case for using leaflet length. South African Journal of Botany 71: 76-80”. The PI formula developed was subsequently used in this research to conduct qualitative and quantitative investigations of plant growth and development in which all data and observations were related directly to the plastochron index. In Chapter 4, the sink to source transition in Pisum sativum L. leaves at different plastochron ages in nodulating plants was investigated using the phloem-mobile fluorescent marker, 5,6-carboxyfluorescein (5,6-CF). The results demonstrated that young leaves remained strong sinks up until LPI 0, after which sink-source transition occurred up to LPI 1.8 and leaflets transitioned to strong source systems by LPI 2.0. A well-developed cross-connected phloem system between paired leaflets in peas, and the petiole and the stem vascular supply was observed. The data presented in the second part of Chapter 4 suggest that the phloem transport between leaflet pairs is independent of the sink/source state of the leaflets, or of movement along the source to sink gradient. The data support the presence of a modular transport system which may ensure re-allocation and balancing between leaflets of the same physiological age and photosynthetic and transport status, thereby load-balancing the local transport system, before exporting to other younger (sink) regions. The investigation of leaf development using the plastochron index (Chapter 5) revealed that the formation of air spaces in the palisade and spongy mesophyll, one of the preparatory events for transition from sink to source state in developing leaves, occurs between LPI 0 and LPI 1 in pea leaflets. Results of the anatomical and ultrastructural study related to PI are presented in Chapter 5. The density of wall ingrowths in transfer cells of minor veins increased with LPI and appeared to be associated with the probable transition to source state and the related potential increase in the production of assimilates for export. The onset of wall ingrowth development in leaflets at LPI 0 provided evidence that sink-to-source transition commences at LPI 0 in P. sativum. Presumably-functional plasmodesmata as well as a few mature sieve elements were evident in class IV veins in the apical region of young and older leaflets at LPI 0. The number of mature sieve elements per vein however, increased with increasing LPI. Most class V veins were still undergoing division at LPI 0 and their sieve elements did not show signs of maturity until LPI 1. The increase in the number of mature metaphloem sieve elements in young, supposedly importing tissue at LPI 0 to older, supposedly exporting tissues at LPI 2 is evidence of the association between phloem maturation and transition from importing to exporting status. In Chapter 6, I report on the effects of elevated CO[subscript 2] on the growth and leaf development of nodulating and non-nodulating Pisum sativum L var. Greenfeast grown under controlled environment of the same nitrogen (6mM) and nitrogen- free nutrient solution conditions. Shortterm exposure to elevated CO[subscript 2] induced rapid plant growth, irrespective of treatment. However, long-term elevated CO[subscript 2] treatment did not affect rate of leaf appearance (RLA) in nodulated plants, irrespective of mineral N supply but enhanced RLA in non- nodulating plants supplied with mineral N. Supplied N resulted in a significant increase in leaflet elongation rate (LfER) under both ambient and elevated CO[subscript 2], but LfER was not significantly affected by nodulation but was increased by high CO[subscript 2]. This suggested that the growth of nodulating P. sativum L may not be significantly affected under CO[subscript 2] levels as high as 1000 μmol mol[superscript -1]. The data suggest that elevated CO[subscript 2] will enhance canopy size, provided adequate soil N is available and more so in non-nodulating plants. This section of the research has been published as “Ade-Ademilua OE, Botha CEJ (2004) The effects of elevated CO[subscript 2] and nitrogen availability supersedes the need for nodulation in peas grown under controlled environmental conditions. South African Journal of Botany 70: 816 – 823”. This thesis demonstrates that the similarity in the qualitative analyses results obtained from plants from different CO[subscript 2], nitrogen and nodulation treatment conditions, highlights the fact that plants of same PI value are at the same developmental state, irrespective of the growth condition. Furthermore, changes in plant structure and function observed under different growth conditions can be related simply to changes in plastochron index. The work presented in this thesis demonstrate that changes in plant structure and function analyzed are related to changes in PI. An important finding of this thesis is that with the use of PI, results can be compiled as a template for predicting the structure- function state of pea plants at any plastochron age, under any growth conditions, before using small representative sample populations

Growth and Essential Oil Yield of African Basil, Ocimum gratissimum, under Light and Water Stress

The growth and essential oil yield of African basil, Ocimum gratissimum L., grown under watered and water-stressed field conditions in full sunlight and natural shade (26.7-44.2% full sunlight) conditions were investigated. Seedlings, 42 days old and growing in a prepared mix contained in plastic bags, were randomly placed in one of four adjoining field plots, one in full sunlight with daily watering, one in full sunlight watered at five day intervals, one in natural shade with daily watering, and one in natural shade watered at five day intervals. Plant height and total leaf area of the African basil were decreased more by water stress than by the light or shade condition. Shade enhanced essential oil content and water stress boosted essential oil content under shade, but reduced oil content under full sunlight. The effects of water stress were only observed in plants in full sunlight. The results demonstrated that African basil will produce relatively high essential oil yields per plant when grown under natural shade, irrespective of water stress and despite poor vegetative growth

What does the movement of the Phloem-mobile symplastic tracer, 5,6-carboxyfluorescein in shoots of Pisum Sativum L. Indicate - the existence of a symplastic transport system? - a bid to answer some puzzling questions

Like other members of the Fabaceae, the minor veins of Pisum are categorized as a closed system termed type 2 minor vein configuration due to the presence of few or no plasmodesmal connections between the sieve element-transfer cell complex (SE-TCC) and the adjacent cells (Gamalei, 1989; van Bel and Gamalei, 1991) Pisum is classified further into the category of type 2 b minor vein configuration due to the presence of transfer cells with the characteristic wall ingrowths in the minor vein phloem (Gamalei, 1989). According to van Bel et al. (1992), there is a correlation between minor vein configuration and phloem loading. However, by reason of low plasmodesmal frequency, the pathway of the flow of assimilates in plants with type 2 minor vein configuration is considered to be apoplasmic (Gamalei, 1989; van Bel and Gamalei, 1991). Therefore, present reports on the movement of phloem-mobile 5,6-carboxyfluorescein, a known symplamically transported compound between pea leaflets raises some doubts on the accession that transport within the phloem in pea is strictly apoplasmic. In this study we look at different points of arguments and try to offer our explanation and conclusions on the transport pathways that are likely to exist in Pisum