Unraveling the Molecular Determinants of Manual Therapy: An Approach to Integrative Therapeutics for the Treatment of Fibromyalgia and Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Application of protocols without parameter standardization and appropriate controls has led manual therapy (MT) and other physiotherapy-based approaches to controversial outcomes. Thus, there is an urgency to carefully define standard protocols that elevate physiotherapy treatments to rigorous scientific demands. One way in which this can be achieved is by studying gene expression and physiological changes that associate to particular, parameter-controlled, treatments in animal models, and translating this knowledge to properly designed, objective, quantitatively-monitored clinical trials (CTs). Here, we propose a molecular physiotherapy approach (MPTA) requiring multidisciplinary teams, to uncover the scientific reasons behind the numerous reports that historically attribute health benefits to MT-treatments. The review focuses on the identification of MT-induced physiological and molecular responses that could be used for the treatment of fibromyalgia (FM) and chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME). The systemic effects associated to mechanical-load responses are considered of particular relevance, as they suggest that defined, low-pain anatomic areas can be selected for MT treatment and yet yield overall benefits, an aspect that might result in it being essential to treat FM. Additionally, MT can provide muscle conditioning to sedentary patients without demanding strenuous physical effort, which is particularly detrimental for CFS/ME patients, placing MT as a real option for integrative medicine programs to improve FM and CFS/ME.This research was funded by the Fundación Universidad Católica de Valencia San Vicente Mártir (UCV) Research Grant Program grant number 2018-121-001.Fisioterapi

Cynoglossum clandestinum Desf. (Boraginaceae), a new especies for the flora of the Catalan Countries

Es dóna a conèixer una localitat nova de Cynoglossum clandestinum Desf. a la comarca de la Vall d’Albaida, que representa una novetat per a la flora dels Països Catalans. Es realitzen comentaris sobre els caràcters morfològics, l’hàbitat i l’estat de conservació. Es discuteix també sobre la seva inclusió en alguna de les categories del Decret 70/2009, que inclou el «Catàleg Valencià d’Espècies de Flora Amenaçada».Cynoglossum clandestinum Desf. has been found in Catalan Countries for the first time. Several comments on the morphological characterization, the habitat and the convervation estatus are reported. We discussed about the need to include it in the Decree 70/2009, in which includes the “Valencian Catalogue of Threatened Plant Species”

Cynoglossum clandestinum Desf. (Boraginaceae), una espècie nova per a la flora dels Països Catalans

Es dóna a conèixer una localitat nova de Cynoglossum clandestinum Desf. a la comarca de la Vall d'Albaida, que representa una novetat per a la flora dels Països Catalans. Es realitzen comentaris sobre els caràcters morfològics, l'hàbitat i l'estat de conservació. Es discuteix també sobre la seva inclusió en alguna de les categories del Decret 70/2009, que inclou el «Catàleg Valencià d'Espècies de Flora Amenaçada».Cynoglossum clandestinum Desf. has been found in Catalan Countries for the first time. Several comments on the morphological characterization, the habitat and the convervation estatus are reported. We discussed about the need to include it in the Decree 70/2009, in which includes the "Valencian Catalogue of Threatened Plant Species"

La falta de homogeneidad del producto (FHP) en las empresas cerámicas y su impacto en la reasignación del inventario

La asignación del producto disponible a prometer (ATP) a pedidos en contextos de fabricación contra almacén (MTS) es de la máxima importancia ya que puede influir en la satisfacción del cliente y en los beneficios de la empresa. Sin embargo, una asignación inicial adecuada, puede pasar a ser inadecuada por diversas razones. En estos casos, es necesaria la reasignación del inventario, la cual será más compleja cuanto más ambiciosos sean los objetivos a alcanzar con ella y mayor el volumen de información a utilizar. En este sentido, cabe destacar que la falta de homogeneidad en el producto (FHP), presente en distintos sectores industriales, provoca la atomización del inventario y aumenta la complejidad de la reasignación, dificultando la obtención de soluciones óptimas. En el presente trabajo se describe la problemática de la FHP, primero de manera genérica, y luego, particularizada a empresas cerámicas MTS. Posteriormente, se identifican las situaciones en las que una determinada asignación de ATP puede dejar de ser adecuada en dicho contexto y se propone la reasignación como una forma de búsqueda de nuevas asignaciones válidas. Finalmente, mediante un caso de estudio de una empresa cerámica, se analiza el impacto de la FHP en cada una de las situaciones identificadas, observando que la FHP provoca alguna de éstas situaciones y complica, en todas ellas, la reasignación del inventario a pedidos.Peer reviewe

Identification of a microRNA signature for the diagnosis of fibromyalgia

Diagnosis of fibromyalgia (FM), a chronic musculoskeletal pain syndrome characterized by generalized body pain, hyperalgesia and other functional and emotional comorbidities, is a challenging process hindered by symptom heterogeneity and clinical overlap with other disorders. No objective diagnostic method exists at present. The aim of this study was to identify changes in miRNA expression profiles (miRNome) of these patients for the development of a quantitative diagnostic method of FM. In addition, knowledge of FM patient miRNomes should lead to a deeper understanding of the etiology and/or symptom severity of this complex disease.Genome-wide expression profiling of miRNAs was assessed in Peripheral Blood Mononuclear Cells (PBMCs) of FM patients (N=11) and population-age-matched controls (N=10) using human v16-miRbase 3D-Gene microarrays (Toray Industries, Japan). Selected miRNAs from the screen were further validated by RT-qPCR. Participating patients were long term sufferers (over 10 years) diagnosed by more than one specialist under 1990 American College of Rheumatology criteria.Microarray analysis of FM patient PBMCs evidenced a marked downregulation of hsa-miR223-3p, hsa-miR451a, hsa-miR338-3p, hsa-miR143-3p, hsa-miR145-5p and hsa-miR-21-5p (4-fold or more). All but the mildest inhibited miRNA, hsa-miR-21-5p, were validated by RT-qPCR. Globally, 20% of the miRNAs analyzed (233/1212) showed downregulation of at least 2-fold in patients. This might indicate a general de-regulation of the miRNA synthetic pathway in FM. No significant correlations between miRNA inhibition and FM cardinal symptoms could be identified. However, the patient with the lowest score for mental fatigue coincided with the mildest inhibition in four of the five miRNAs associated with the FM-group.We propose a signature of five strikingly downregulated miRNAs (hsa-miR223-3p, hsa-miR451a, hsa-miR338-3p, hsa-miR143-3p and hsa-miR145-5p) to be used as biomarkers of FM. Validation in larger study groups is required before the results can be transferred to the clinic.The study was funded by the Fundación Universidad Católica de Valencia “San Vicente Mártir”(UCV) Research Grant Program (2011-011-02, 2011-011-04 and 2012-011-008) the Fundación Cátedra Umivale at the UCV and the 3D-Gene International Competition Award to EOMedicin

Microrna Expression Profile in Fibromyalgia Patients: A Pilot Matched Case Control Study

The objective of this study was to determine whether an association between an altered miRNAs expression profile and fibromyalgia exists.Medicin

Evaluation of the MODIS Albedo Product over a Heterogeneous Agricultural Area

In this article, the Moderate Resolution Imaging Spectroradiometer (MODIS) Bidirectional Reflectance Distribution Function (BRDF)/Albedo product (MCD43) is evaluated over a heterogeneous agricultural area in the framework of the Earth Observation: Optical Data Calibration and Information Extraction (EODIX) project campaign, which was developed in Barrax (Spain) in June 2011. In this method, two models, the RossThick-LiSparse-Reciprocal (RTLSR) (which corresponds to the MODIS BRDF algorithm) and the RossThick-Maignan-LiSparse-Reciprocal (RTLSR-HS), were tested over airborne data by processing high-resolution images acquired with the Airborne Hyperspectral Scanner (AHS) sensor. During the campaign, airborne images were retrieved with different view zenith angles along the principal and orthogonal planes. Comparing the results of applying the models to the airborne data with ground measurements, we obtained a root mean square error (RMSE) of 0.018 with both RTLSR and RTLSR-HS models. The evaluation of the MODIS BRDF/Albedo product (MCD43) was performed by comparing satellite images with AHS estimations. The results reported an RMSE of 0.04 with both models. Additionally, taking advantage of a homogeneous barley pixel, we compared in situ albedo data to satellite albedo data. In this case, the MODIS albedo estimation was (0.210 +/- 0.003), while the in situ measurement was (0.204 +/- 0.003). This result shows good agreement in regard to a homogeneous pixel