Exogenous contrast agents for thermoacoustic imaging: an investigation into the underlying sources of contrast.

Thermoacoustic imaging at microwave excitation frequencies is limited by the low differential contrast exhibited by high water content tissues. To overcome this, exogenous thermoacoustic contrast agents based on gadolinium compounds, iron oxide, and single wall carbon nanotubes have previously been suggested and investigated. However, these previous studies did not fully characterize the electric, magnetic, and thermodynamic properties of these agents thus precluding identification of the underlying sources of contrast. To address this, measurements of the complex permittivity, complex permeability, DC conductivity, and Grüneisen parameter have been made. These measurements allowed the origins of the contrast provided by each substance to be identified

Effects of Petroleum Products on the Chlorophyll Accumulation in Barbula lambarenensis

The effects of petroleum products: Petroleum Motor Spirit (petrol, PMS), Automobile Gas Oil (diesel. AGO), Dual Purpose Kero (kerosene, DPK), were investigated on the chlorophyll accumulation in Barbula lambarenensis. This plant with its substrates were collected from its natural populations, taken to the screen house and divided into ten regimes widely separated. Each of these regimes was irrigated with borehole water for two weeks, thrice a week to bring about hydration. The three petroleum products were separately diluted with borehole water in 1:1 ratio to give 50% concentrations. Moreover, 100% of these products were also used .The 50% concentrations were separately used to irrigate the first three regimes, A, B and C for 20 weeks .The D, E and F regimes were irrigated with 100% kerosene, petrol and diesel respectively for the 2 weeks and later with borehole water for twenty weeks while the G regime was irrigated with borehole water (Control). Irrigations were done thrice a week. Samples of B. lambarenensis were collected fortnightly for chlorophyll extraction and the chlorophyll a, b and total chlorophyll content determined with the aid of spectrophotometer .It was observed that the 50% treated moss plants had greater chlorophyll a, b and total chlorophyll than the control but their concentrations were statistically the same. The 100% treatment gave greater chlorophyll a content than the control, PMS treatment had greater chlorophyll b than the control but the reverse were for DPK and AGO treated moss plant. However, all these values were the same statistically. The same results were found for the total chlorophyll except that the DPK, AGO and Control treatments had the same value statistically while DPK, Control and PMS treatments were the same statistically. The 50% treatments had worse effects than the 100% followed by borehole water treatments. Furthermore, the two concentrations had the same effects on the chlorophyll contents but DPK and AGO had significantly greater chlorophyll b concentrations in 50% treated regimes than 100% treatments at 5% level of significance. Petroleum products damaged the chlorophyll apparatus of the plant making the treated to produce greater chlorophyll contents than the Control

Photoacoustic tomography using orthogonal Fabry-Pérot sensors

Fabry–Pérot sensors have been used to produce in-vivo photoacoustic images of exquisite quality. However, for ease of construction and interrogation, they are produced in a planar form. Planar arrays suffer from a limited detection aperture, which leads to artifacts in the reconstruction of the initial pressure distribution. Here, an L-shaped detection geometry is described that allows a greater field of view by placing a second planar array orthogonal to the first. This captures data from the deeper lying regions of interest and mitigates the limited view, thus reducing artifacts in the reconstructed initial pressure distribution

Photoacoustic imaging using an 8-beam Fabry-Perot scanner

The planar Fabry Perot (FP) photoacoustic scanner has been shown to provide exquisite high resolution 3D images of soft tissue structures in vivo to depths up to approximately 10mm. However a significant limitation of current embodiments of the concept is low image acquisition speed. To increase acquisition speed, a novel multi-beam scanner architecture has been developed. This enables a line of equally spaced 8 interrogation beams to be scanned simultaneously across the FP sensor and the photoacoustic signals detected in parallel. In addition, an excitation laser operating at 200Hz was used. The combination of parallelising the detection and the high pulse repetition frequency (PRF) of the excitation laser has enabled dramatic reductions in image acquisition time to be achieved. A 3D image can now be acquired in 10 seconds and 2D images at video rates are now possible. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

Accelerated High-Resolution Photoacoustic Tomography via Compressed Sensing

Current 3D photoacoustic tomography (PAT) systems offer either high image quality or high frame rates but are not able to deliver high spatial and temporal resolution simultaneously, which limits their ability to image dynamic processes in living tissue. A particular example is the planar Fabry-Perot (FP) scanner, which yields high-resolution images but takes several minutes to sequentially map the photoacoustic field on the sensor plane, point-by-point. However, as the spatio-temporal complexity of many absorbing tissue structures is rather low, the data recorded in such a conventional, regularly sampled fashion is often highly redundant. We demonstrate that combining variational image reconstruction methods using spatial sparsity constraints with the development of novel PAT acquisition systems capable of sub-sampling the acoustic wave field can dramatically increase the acquisition speed while maintaining a good spatial resolution: First, we describe and model two general spatial sub-sampling schemes. Then, we discuss how to implement them using the FP scanner and demonstrate the potential of these novel compressed sensing PAT devices through simulated data from a realistic numerical phantom and through measured data from a dynamic experimental phantom as well as from in-vivo experiments. Our results show that images with good spatial resolution and contrast can be obtained from highly sub-sampled PAT data if variational image reconstruction methods that describe the tissues structures with suitable sparsity-constraints are used. In particular, we examine the use of total variation regularization enhanced by Bregman iterations. These novel reconstruction strategies offer new opportunities to dramatically increase the acquisition speed of PAT scanners that employ point-by-point sequential scanning as well as reducing the channel count of parallelized schemes that use detector arrays

A review: Aedes-borne arboviral infections, controls and Wolbachia-based strategies

Arthropod-borne viruses (Arboviruses) continue to generate significant health and economic burdens for people living in endemic regions. Of these viruses, some of the most important (e.g., dengue, Zika, chikungunya, and yellow fever virus), are transmitted mainly by Aedes mosquitoes. Over the years, viral infection control has targeted vector population reduction and inhibition of arboviral replication and transmission. This control includes the vector control methods which are classified into chemical, environmental, and biological methods. Some of these control methods may be largely experimental (both field and laboratory investigations) or widely practised. Perceptively, one of the biological methods of vector control, in particular, Wolbachia-based control, shows a promising control strategy for eradicating Aedes-borne arboviruses. This can either be through the artificial introduction of Wolbachia, a naturally present bacterium that impedes viral growth in mosquitoes into heterologous Aedes aegypti mosquito vectors (vectors that are not natural hosts of Wolbachia) thereby limiting arboviral transmission or via Aedes albopictus mosquitoes, which naturally harbour Wolbachia infection. These strategies are potentially undermined by the tendency of mosquitoes to lose Wolbachia infection in unfavourable weather conditions (e.g., high temperature) and the inhibitory competitive dynamics among co-circulating Wolbachia strains. The main objective of this review was to critically appraise published articles on vector control strategies and specifically highlight the use of Wolbachia-based control to suppress vector population growth or disrupt viral transmission. We retrieved studies on the control strategies for arboviral transmissions via arthropod vectors and discussed the use of Wolbachia control strategies for eradicating arboviral diseases to identify literature gaps that will be instrumental in developing models to estimate the impact of these control strategies and, in essence, the use of different Wolbachia strains and feature

Stem cell delivery to kidney via minimally invasive ultrasound-guided renal artery injection in mice

Cell-based therapies are promising treatments for various kidney diseases. However, the major hurdle in initiating therapeutic responses is the inefficiency of injection routes to deliver cells to the kidney parenchyma. Systemic injection, such as intravenous injection only delivers a small proportion of cells to the kidney. Whereas direct delivery, such as renal artery injection requires surgical procedures. A minimally invasive renal artery injection was therefore developed to enhance cell delivery to kidney. In this study, luciferase expressing human adipocyte derived stem cells (ADSC) were labelled with gold nanorods (GNR) and injected into the renal artery using ultrasound guidance. The ADSCs were tracked using bioluminescence and photoacoustic imaging serially over 7 days. Imaging confirmed that the majority of signal was within the kidney, indicative of successful injection and that the cells remained viable for 3 days. Histology showed co-localization of GNRs with ADSC staining throughout the kidney with no indication of injury caused by injection. These findings demonstrate that ultrasound-guided renal artery injection is feasible in mice and can successfully deliver a large proportion of cells which are retained within the kidney for 3 days. Therefore, the techniques developed here will be useful for optimising cell therapy in kidney diseases

INTRODUCTION OF SOIL TEST KIT TO SMALL-HOLDER COCOA FARMERS IN SOUTH-WESTERN NIGERIA

ABSTRACT Soil nutrients depletion of cocoa plantations is one of the causes of low cocoa production in Nigeria. Soil testing to determine the appropriate type and rate of fertilizer to boost cocoa bean yield suggests itself. Conventional soil testing is not within the reach of smallholder cocoa farmers because of its cost and other logistics but soil testing is necessary to avert negative consequences of blanket fertilizer recommendation. Therefore the objective of this study is to introduce an affordable soil test kit for soil testing to smallholders cocoa farmers. The study was carried out in Ondo and Osun States of Nigeria. Multi-stage random sampling technique was used to select eighty two cocoa farmers from the study area. Information was collected from the selected cocoa farmers with the aid of structured questionnaire and the data were analysed using descriptive statistics. The result shows that only 20.73% of the respondent farmers carry out soil test on their farms while 85.36% of the farmers know the relevance of soil test. The result also revealed that 98.78% of the farmers are ready to acquire the soil test kit. The study therefore recommends that (i) The soil test kit should be included among the subsidized inputs that government gives to farmers from time to time and (ii) private entrepreneurs could also procure and make the kits available to the farmers

Monitoring neovascularization and integration of decellularized human scaffolds using photoacoustic imaging

Tissue engineering is a branch of regenerative medicine that aims to manipulate cells and scaffolds to create bioartificial tissues and organs for patients. A major challenge lies in monitoring the blood supply to the new tissue following transplantation: the integration and neovascularization of scaffolds in vivo is critical to their functionality. Photoacoustic imaging (PAI) is a laser-generated ultrasound-based technique that is particularly well suited to visualising microvasculature due to the high optical absorption of haemoglobin. Here, we describe an early proof-of-concept study in which PAI in widefield tomography mode is used to image biological, decellularized human tracheal scaffolds. We found that PAI allowed the longitudinal tracking of scaffold integration into subcutaneous murine tissue with high spatial resolution at depth over an extended period of time. The results of the study were consistent with post-imaging histological analyses, demonstrating that PAI can be used to non-invasively monitor the extent of vascularization in biological tissue-engineered scaffolds. We propose that this technique may be a valuable tool for studies designed to test interventions aimed at improving the speed and extent of scaffold neovascularization in tissue engineering. With technological refinement, it could also permit in vivo monitoring of revascularization in patients, for example to determine timing of heterotopic graft transfer