Influence of dietary protein deficiency on amino acid and fatty acid composition in tilapia, Oreochromis niloticus, fingerlings.

Abstract The influence of dietary protein deficiency on the amino acid and fatty acid compositions of tilapia (Oreochromis niloticus) fingerlings was studied. Two experimental diets (0.81% and 33.32% protein, dry matter) were prepared. The protein content of fish fed diet 1 (0.81% protein) decreased from 57.14% to 49.18% in eight weeks. Fish fed diet 2 (33.32% protein) had higher protein and amino acid contents. The lipid content of fish fed diet 1 was higher than that of fish fed diet 2, suggesting that carbohydrates transformed into lipids. The levels of fatty acids 16:0 and 18:2 n-6 in fish fed diet 1 remained nearly unchanged and did not reflect the diet, demonstrating that fatty acids in diet 1 may not have been incorporated into the triglycerides of the tissues. Possible impairment of lipid secretion from the liver, caused by depletion of protein in the blood lipoprotein, may have affected the transport of lipids to the muscles. A dietary protein deficiency results not only in a deficiency of essential amino acids in the body but also affects transport and storage of lipids within the fish

Nutrient Composition of some Tropical Legumes Capable of Substituting Fish Meal in Fish Diets

Sword beans (Canavalia gladiata); Jackbean (Canavalia enciformis (L)); Mucuna bean (Mucuna pruriens); Mucuna cochiunensis; Bambara (Voandzeia subterranea) and Limabean (Phaseolus lunatus) are the tropical legumes considered in this paper. They have been used in the feed of ruminants but very scarcely considered in fish feed. Information about their nutrient composition are also scarce. Results from this study show that the protein contents of the test seeds ranged from 19.94% dry matter (DM), (Bambara) to 36.95% DM (Mucuna cochiunensis). Considering the high protein level required by fish for maximum growth and the presence of some ANFs, the seeds may not be able to be used in isolation without supplementing them with other food stuffs having higher protein value. The relatively high content of Nitrogen Free Extract (+ fibre) seem to suggest that the test seeds can be used in a semi-intensive setting to supply carbohydrate in fish diets. The seeds contain considerable amount of linoleic acid (18:2 n-6). The highest occurring in Lima beans. Sword beans and Jack beans are rich in oleic acid (18:1n-9). Palmatic acid (16:0) is high, while stearic acid (18:0) and myristic acid (14:0) are low. The amino acid compositions of the test seeds are not very adequate. Sword beans had a better amino acid profile though it seems deficient in some of the amino acids. The amino acid contents of Jack bean, Mucuna bean, Bambara and Lima bean look inadequate to provide a possible alternative to fish meal on individual basis. If to be used in fish feed formulation, combinating them with other protein sources, possessing higher contents of the limiting amino acids is strongly suggested. The potentials of these seeds in fish feed formulation seem high

Influences Of Dietary Protein Deficiency On Amino Acid And Fatty Acid Composition In Tilapia, Oreochromis Niloticus, Fingerlings

The influence of dietary protein deficiency on the amino acid and fatty acid compositions of tilapia (Oreochromis niloticus) fingerlings was studied. Two experimental diets (0.81% and 33.32% protein, dry matter) were prepared. The protein content of fish fed diet 1 (0.81% pro- tein) decreased from 57.14% to 49.18% in eight weeks. Fish fed diet 2 (33.32% protein) had higher protein and amino acid contents. The lipid content of fish fed diet 1 was higher than that of fish fed diet 2, suggesting that carbohydrates transformed into lipids. The levels of fatty acids 16:0 and 18:2 n-6 in fish fed diet 1 remained nearly unchanged and did not reflect the diet, demonstrating that fatty acids in diet 1 may not have been incorporated into the triglycerides of the tissues. Possible impairment of lipid secretion from the liver, caused by depletion of protein in the blood lipoprotein, may have affected the transport of lipids to the muscles. A dietary pro- tein deficiency results not only in a deficiency of essential amino acids in the body but also affects transport and storage of lipids within the fish

Alternative Protein Sources As Substitutes For Fishmeal In The Diet Of Young Tilapia Oreochromis Niloticus (LINN.)

Fifteen test diets were theoretically formulated to contain 33.32% dietary protein, dry matter, using a mixture of alternative protein sources and fishmeal in various proportions. Test diets were fed to fingerlings (initial weight 3.2±0.2 g) in triplicate tanks for eight weeks at 5% body weight per day in three portions. At the end of each experimental phase, fish carcass from the feeding groups was homogenized. Freeze-dried samples of fish at the beginning and end of the experiments as well as samples of the test diets were analyzed for proximate composition. Fish fed diet 1 containing 43% fishmeal recorded the highest weight gain, highest specific growth rate (SGR, 3.46%/d) and lowest food conversion ratio (FCR, 1.11). When part of the fishmeal was substituted with 18% soy- bean meal and 5% blood meal (diet 8), SGR and FCR were 3.02%/d and 1.34, respectively, not significantly different from diet 1 at 0.05 probability. Results showed that proper combination of alternative protein sources can provide 42-45% of the protein required by Oreochromis niloticus (33.32% dietary protein, dry matter). In such a combination, soybean meal can replace up to 25% of the fishmeal as a protein source. Blood meal exceeding 6%, groundnut cake beyond 10%, soy- bean meal above 20%, and wheat bran beyond 10% retarded fish growth. High mortality was gen- erally observed when these levels were exceeded, even in only one of the protein sources

Housefly Maggot Meal as a Potent Bioresource for Fish Feed to Facilitate Early Gonadal Development in <i>Clarias gariepinus</i> (Burchell,1822)

Food security is threatened by the global increase of human population, climate change, inequality of wealth distribution, the state of natural resources, peace and sustainability, among others. Aquaculture has become an important part of the food supply, hence contributing to food security. Sustainable fish production, which represents an important affordable protein supply, should therefore involve feed formulation and production techniques that are not dependent on fish meal as the protein source. It is on this basis that bioeconomy becomes very important to promote the resourceful use of biomass for feed, food, biomaterials, and bioenergy. This study presents housefly maggot meal as an efficient alternative protein source to fishmeal and a valuable bioresource. Clarias gariepinus fingerlings (male and female) were fed maggot meal supplemented diets for 24 weeks and we assessed their growth and gonadal development. The findings of this study revealed that maggot-meal-enhanced diets promoted growth and faster gonadal development in fish. Maggot meal represents a quality alternative protein source to fish meal and an excellent bioresource, which can help us attain sustainable aquaculture production

Housefly Maggot Meal as a Potent Bioresource for Fish Feed to Facilitate Early Gonadal Development in Clarias gariepinus (Burchell,1822)

Food security is threatened by the global increase of human population, climate change, inequality of wealth distribution, the state of natural resources, peace and sustainability, among others. Aquaculture has become an important part of the food supply, hence contributing to food security. Sustainable fish production, which represents an important affordable protein supply, should therefore involve feed formulation and production techniques that are not dependent on fish meal as the protein source. It is on this basis that bioeconomy becomes very important to promote the resourceful use of biomass for feed, food, biomaterials, and bioenergy. This study presents housefly maggot meal as an efficient alternative protein source to fishmeal and a valuable bioresource. Clarias gariepinus fingerlings (male and female) were fed maggot meal supplemented diets for 24 weeks and we assessed their growth and gonadal development. The findings of this study revealed that maggot-meal-enhanced diets promoted growth and faster gonadal development in fish. Maggot meal represents a quality alternative protein source to fish meal and an excellent bioresource, which can help us attain sustainable aquaculture production