ラット腹腔肥満細胞のCa(2+) influxおよびヒスタミン遊離に対する抗アレルギー薬アゼラスチンの抑制効果について

The effect of antiallergic agent, azelastine, was examined in immunogical secretory process of mast cells. 1. Azelastine significantly inhibited (45)Ca uptake by mast cells stimulated by antigen, and the maximum inhibition was attained at a concentration of 50 μg/ml showing approximately 30.5 % inhibition. 2. Azelastine also inhibite the release of histamine from mast cells by antigen. The maximal inhibitory rate was 38.1%. The effect of azelastine on (45)Ca uptake and histamine release was dose-dependent, and compatible at employed cocentration of the agent. 3. Tachpylaxis to azelastine was not found in this experimental system.抗原刺激時の,ラット腹腔肥満細胞のCa(2+) uptakeおよびヒスタミン遊離に対する,抗アレルギー薬アゼラスチンの抑制効果について検討を加えた｡その結果,1.アゼラスチンは抗原刺激時の肥満細胞のCa(2+) uptakeに対して濃度依存性の抑制効果を示した｡2.同様に,抗原刺激時の肥満細胞からのヒスタミン遊離に対しても,濃度依存性の抑制効果を示した｡3.アゼラスチンの再暴露による抑制効果の減弱傾向は見られず,アゼラスチンでは　tachyphylaxisは観察されなかった

A Study on the Planning and Scheduling of Production System Considering Demand Changes

In this paper, we studied a planning and scheduling of production system considering demand changes. In the proposed system, planning part determines lot-size and amount of jobs in production. On the other hand, scheduling part determines the production sequence of jobs. In order to treat with the demand changes, both planning and scheduling should work well simultaneously. In the proposed system, preset and real time production control system is newly constructed from the view point of adaptive control. In the system, production planning is modified when the difference between production amount and demand becomes large. Moreover, production schedule is regenerated when the determined schedule is deviated from the prospected one. The scheduling system is characterized as the autonomous decentralized optimization system where each job works as agent and agent searches its appropriate starting time of processing. The effectiveness of the proposed system is confirmed by numerical examples

A Rare Case of Gastrojejunocutaneous Fistula after Percutaneous Endoscopic Gastrostomy

We report a rare case of fistulation to the jejunum after percutaneous endoscopic gastrostomy (PEG). An 85-year-old man with previous cerebral infarction and swallowing disturbance underwent PEG. Nine months later, he developed a high fever and discharge around the gastrostomy button. He was diagnosed with aspiration pneumonia, and administered antibiotic therapy. Examination showed digestive fluid around the gastrostomy button. Gastrointestinal contrast-enhanced CT revealed a gastrojejunocutaneous fistula. The button was removed, and the fistula closed naturally. PEG was performed again. The patient’s postoperative course was uneventful. Gastrojejunocutaneous fistula should be considered in cases involving increased discharge from a gastrostomy fistula

Evidence for the possible involvement of the P2Y6 receptor in Ca2+ mobilization and insulin secretion in mouse pancreatic islets

Subtypes of purinergic receptors involved in modulation of cytoplasmic calcium ion concentration ([Ca2+]i) and insulin release in mouse pancreatic β-cells were examined in two systems, pancreatic islets in primary culture and beta-TC6 insulinoma cells. Both systems exhibited some physiological responses such as acetylcholine-stimulated [Ca2+]i rise via cytoplasmic Ca2+ mobilization. Addition of ATP, ADP, and 2-MeSADP (each 100 µM) transiently increased [Ca2+]i in single islets cultured in the presence of 5.5 mM (normal) glucose. The potent P2Y1 receptor agonist 2-MeSADP reduced insulin secretion significantly in islets cultured in the presence of high glucose (16.7 mM), whereas a slight stimulation occurred at 5.5 mM glucose. The selective P2Y6 receptor agonist UDP (200 µM) transiently increased [Ca2+]i and reduced insulin secretion at high glucose, whereas the P2Y2/4 receptor agonist UTP and adenosine receptor agonist NECA were inactive. [Ca2+]i transients induced by 2-MeSADP and UDP were antagonized by suramin (100 µM), U73122 (2 µM, PLC inhibitor), and 2-APB (10 or 30 µM, IP3 receptor antagonist), but neither by staurosporine (1 µM, PKC inhibitor) nor depletion of extracellular Ca2+. The effect of 2-MeSADP on [Ca2+]i was also significantly inhibited by MRS2500, a P2Y1 receptor antagonist. These results suggested that P2Y1 and P2Y6 receptor subtypes are involved in Ca2+ mobilization from intracellular stores and insulin release in mouse islets. In beta-TC6 cells, ATP, ADP, 2-MeSADP, and UDP transiently elevated [Ca2+]i and slightly decreased insulin secretion at normal glucose, while UTP and NECA were inactive. RT-PCR analysis detected mRNAs of P2Y1 and P2Y6, but not P2Y2 and P2Y4 receptors

好塩基球からのヒスタミン遊離に関する研究. 1 自動分析装置による全血からのヒスタミン遊離の測定

Histamine released from whole blood was determined by an automated fiuorometric histamine analysis system. The increased release of histamine from basophils by anti-IgE was observed in ten healthy subjects and 12 extrinsic asthma patients, while the release in 11 intrinsic asthma patients was significantly less as compared to that in healthy and extrinsic asthma subjects. House dust extract caused a significant increase in the histamine release from basophils of the extrinsic asthma patients who are sensitive to house dust. It was concluded from this study that histamine released from basophils could be easily determined by an automated analysis system and that the method is useful for the diagnosis and study of allergy.ヒスタミン自動分析装置により,健康人10名,気管支喘息23例の全血からのヒスタミン遊離を測定した. 抗ヒトIgEを添加した際のヒスタミン遊離は,健康人および外因性気管支喘息症例では有意の増加傾向を示したが,一方内因性喘息症例では遊離増加はほとんどみられなかった. ハウスダスト抗原添加では,ハウスダストが抗原である気管支喘息症例においてのみ全血からの有意のヒスタミン遊離の増加が観察された. 以上の結果より,ヒスタミン自動分析装置による全血からの遊離ヒスタミンの測定は,気管支喘息の病態解明の1手段として極めて有用であると考えられる

Spa therapy for patients with chronic obstructive lung disease

Thirty-six patients with chronic obstructive lung disease (34 cases with bronchial asthma, one case with chronic bronchiolitis and one case with pulmonary emphysema) have received spa therapy. Clinical effects of sa therapy on patients with bronchial asthma depended on patient age and asthma types classified by allergic reactions and clinical symptoms. Spa therapy was effective in the cases with ages more than 31 years and the cases with non-atopic type of bronchial asthma. Regarding asthma type classified by clinical symptoms, spa therapy was more effective in the cases with bronchiolar obstructive type and the cases with bronchospasm + hypersecretion type than in the cases with bronchospasm type of bronchial asthma

Inhibitory effect of the CA2+ antagonist nifedipine on histamine release from rat peritoneal mast cells.

45Ca uptake and histamine release was examined in mast cells from rats sensitized with ovalbumin and Bordetella Bertussis as an adjuvant. The uptake of 45Ca by the mast cells was significantly increased by stimulation with ovalbumin as was the release of histamine from the mast cells. Nifedipine, a calcium antagonist, inhibited the increase in both 45Ca uptake and histamine release stimulated by ovalbumin, though the effect on 45Ca uptake was stronger than that on histamine release.</p