Thin KAPTON polyimide films vacuum formed at high temperature retain their shape at temperatures to 450 K

Purpose of investigation was to identify candidate materials for self-evacuating multilayer insulation systems to be used on liquid hydrogen tanks on space shuttle, which would survive re-entry temperatures and mechanical and thermal cycling of one hundred flights

Natural transformation of the filamentous cyanobacterium Phormidium lacuna

Research for biotechnological applications of cyanobacteria focuses on synthetic pathways and bioreactor design, while little effort is devoted to introduce new, promising organisms in the field. Applications are most often based on recombinant work, and the establishment of transformation can be a risky, time-consuming procedure. In this work we demonstrate the natural transformation of the filamentous cyanobacterium Phormidium lacuna and insertion of a selection marker into the genome by homologous recombination. This is the first example for natural transformation filamentous non-heterocystous cyanobacterium. We found that Phormidium lacuna is polyploid, each cell has about 20–90 chromosomes. Transformed filaments were resistant against up to 14 mg/ml of kanamycin. Formerly, natural transformation in cyanobacteria has been considered a rare and exclusive feature of a few unicellular species. Our finding suggests that natural competence is more distributed among cyanobacteria than previously thought. This is supported by bioinformatic analyses which show that all protein factors for natural transformation are present in the majority of the analyzed cyanobacteria

The assessment of water use and reuse through reported data: A US case study

Increasing demands for freshwater make it necessary to find innovative ways to extend the life of our water resources, and to manage them in a sustainable way. Indirect water reuse plays a role in meeting freshwater demands but there is limited documentation of it. There is a need to analyze its current status for water resources planning and conservation, and for understanding how it potentially impacts human health. However, the fact that data are archived in discrete uncoordinated databases by different state and federal entities, limits the capacity to complete holistic analysis of critical resources at large watershed scales. Humans alter the water cycle for food production, manufacturing, energy production, provision of potable water and recreation. Ecosystems services are affected at watershed scales but there are also global scale impacts from greenhouse gas emissions enabled by access to cooling, processing and irrigation water. To better document these issues and to demonstrate the utility of such an analysis, we studied the Wabash River Watershed located in the U.S. Midwest. Data for water extraction, use, discharge, and river flow were collected, curated and reorganized in order to characterize the water use and reuse within the basin. Indirect water reuse was estimated by comparing treated wastewater discharges with stream flows at selected points within the watershed. Results show that during the low flow months of July–October, wastewater discharges into the Wabash River basin contributed 82 to 121% of the stream flow, demonstrating that the level of water use and unplanned reuse is significant. These results suggest that intentional water reuse for consumptive purposes such as landscape or agricultural irrigation could have substantial ecological impacts by diminishing stream flow during vulnerable low flow periods

Time series analysis of water use and indirect reusewithin a HUC-4 basin (Wabash) over a nine year period

Anthropogenic water use and reuse represent major components of the water cycle. In the context of climate change, water reuse and recycling are considered necessary components for an integrated water management approach. Unplanned, or de facto, indirect water reuse occurs in most of the U.S. river systems, however, there is little real-time documentation of it. Despite the fact that there are national and state agencies that systematically collect data on water withdrawals and wastewater discharges, their databases are organized and managed in a way that makes it challenging to use them for water resource management analysis. The ability to combine reported water data to perform large scale analysis about water use and reuse is severely limited. In this paper, we apply a simple but effective methodology to complete a time series watershed-scale analysis of water use and unplanned indirect reuse for the Wabash River Watershed. Results document the occurrence of indirect water reuse, ranging from 3% to 134%, in a water-rich area of the U.S. The time series analysis shows that reported data effectively describe the water use trends through nine years, from 2009 to 2017, clearly reflecting both anthropogenic and natural events in the watershed, such as the retirement of thermoelectric power plants, and the occurrence of an extreme drought in 2012. We demonstrate the feasibility and significance of using available water datasets to perform large scale water use analysis, describe limitations encountered in the process, and highlight areas for improvement in water data management

Division of Student Affairs: Centennial History

This departmental history was written on the occasion of the UND Centennial in 1983.https://commons.und.edu/departmental-histories/1110/thumbnail.jp

Proteomic responses to gold(III)-toxicity in the bacterium Cupriavidus metallidurans CH34

Accepted 11th October 2016The metal-resistant β-proteobacterium Cupriavidus metallidurans drives gold (Au) biomineralisation and the (trans)formation of Au nuggets largely via unknown biochemical processes, ultimately leading to the reductive precipitation of mobile, toxic Au(i/iii)-complexes. In this study proteomic responses of C. metallidurans CH34 to mobile, toxic Au(iii)-chloride are investigated. Cells were grown in the presence of 10 and 50 μM Au(iii)-chloride, 50 μM Cu(ii)-chloride and without additional metals. Differentially expressed proteins were detected by difference gel electrophoresis and identified by liquid chromatography coupled mass spectrometry. Proteins that were more abundant in the presence of Au(iii)-chloride are involved in a range of important cellular functions, e.g., metabolic activities, transcriptional regulation, efflux and metal transport. To identify Au-binding proteins, protein extracts were separated by native 2D gel electrophoresis and Au in protein spots was detected by laser absorption inductively coupled plasma mass spectrometry. A chaperon protein commonly understood to bind copper (Cu), CupC, was identified and shown to bind Au. This indicates that it forms part of a multi-metal detoxification system and suggests that similar/shared detoxification pathways for Au and Cu exist. Overall, this means that C. metallidurans CH34 is able to mollify the toxic effects of cytoplasmic Au(iii) by sequestering this Au-species. This effect may in the future be used to develop CupC-based biosensing capabilities for the in-field detection of Au in exploration samples.Carla M. Zammit, Florian Weiland, Joël Brugger, Benjamin Wade, Lyron Juan Winderbaum, Dietrich H. Nies, Gordon Southam, Peter Hoffmann and Frank Reit

Recurrent scoliosis one year after surgical correction

A year after anterolateral spondylodesis for progressive scoliosis, the patient showed a flexion gait pattern with recurrent deformity, due to late infection. Surgical debridement resolved all symptoms. Whereas most postoperative infections occur after posterior spondylodesis and present with back pain and mild increase of infection parameters, late infection after anterolateral approach is rare. In this case the patient did not present with the classic symptoms

Nonoperative Treatment of Thoracic and Lumbar Spine Fractures:A Prospective Randomized Study of Different Treatment Options

Objectives: To evaluate and compare nonoperative treatment methods for traumatic thoracic and lumbar compression fractures and burst fractures.Design: Prospective randomized controlled trial with long-term follow-up.Setting: Two general hospitals in the Netherlands.Patients/Participants: Patients with a traumatic thoracic or lumbar spine fracture, without neurologic damage, with less than 50% loss of height of the anterior column and less than 30% reduction of the spinal canal were included.Intervention: Patients in the compression group were randomized to physical therapy and postural instructions, a brace for 6 weeks, or a Plaster of Paris cast for 6 or 12 weeks. Patients in the burst group received a brace or a Plaster of Paris cast, both for 12 weeks.Main Outcome Measurements: Follow-up examinations included radiographs, Visual Analogue Scores for toleration of treatment and persistent pain, and an Oswestry Disability Index at long-term follow-up.Results: There were 133 patients: 108 in the compression group and 25 in the burst group. For compression fractures, physical therapy and brace were considered the most tolerable. Brace therapy scored significantly better on the Visual Analogue Scores for residual pain and on the Oswestry Disability Index. None of the treatments had any significant effect on the residual deformity measurements. For burst fractures, no significant differences were found.Conclusions: Brace treatment with supplementary physical therapy is the treatment of choice for patients with compression fractures of the thoracic and lumbar spine. Furthermore, more than 20% of all patients had moderate or severe back pain at long-term follow-up.</p