Annual ryegrass toxicity research summary of experiment results.

Experiments were set up to screen a range of herbicides which might give improved control of Anguina agrostis over the currently recommended paraquat. Two experiments examined Roundup, Fusilade and Sertin at two rates and two experiments screened eight herbicides at five rates from two x label recommendation to 0. 99 x Standard treatment was paraquat at 550 ml. Plots were 3 m x 30 m in the first two experiments and 3 x 5 m (per dilution) in the second. The herbicides were applied on 4/9, 17/9, 24/9, 1/10 and 9/10 (Zadoks stages 32 - 58). There were three replications with 54 nil control plots. Efficacy was measured as galls/m 2 recovered at maturity. Table 1 and Table 2 show the results from two sites, Dumbleyung and Corrigin respectively. No analysis has yet been undertaken and variability is extreme. Unless some form of covariance analysis using nil plots can be undertaken, no useful information can be obtained. Table 3 shows results from a single replication of a trial comparing nine herbicides applied by log dilution sprayer. Only two rates have been processed so far: the highest and lowest

Wanted dead or alive? The tradeoff between in-vivo versus ex-vivo MR brain imaging in the mouse

High-resolution MRI of the mouse brain is gaining prominence in estimating changes in neuroanatomy over time to understand both normal developmental as well as disease processes and mechanisms. These types of experiments, where a change in time is to be captured as accurately as possible using imaging, face multiple experimental design choices. Chief amongst these choices is whether to image ex-vivo, where superior resolution and contrast are available, or in-vivo, where resolution and contrast are lower but the animal can be followed longitudinally. Here we explore this tradeoff by first estimating the sources of variability in anatomical mouse MRI and then, using statistical simulations, provide power analyses of these experiment design choices

Vanadium (β-(Dimethylamino)ethyl)cyclopentadienyl Complexes with Diphenylacetylene Ligands

Reduction of the V(III) (β-(dimethylamino)ethyl)cyclopentadienyl dichloride complex [η5:η1-C5H4(CH2)2NMe2]VCl2(PMe3) with 1 equiv of Na/Hg yielded the V(II) dimer {[η5:η1-C5H4(CH2)2NMe2]V(µ-Cl)}2 (2). This compound reacted with diphenylacetylene in THF to give the V(II) alkyne adduct [η5:η1-C5H4(CH2)2NMe2]VCl(η2-PhC≡CPh). Further reduction of 2 with Mg in the presence of diphenylacetylene resulted in oxidative coupling of two diphenylacetylene groups to yield the diamagnetic, formally V(V), bent metallacyclopentatriene complex [η5:η1-C5H4(CH2)2NMe2]V(C4Ph4).

Preliminary Results: Complementary C4:C3 Grazing Systems

Native warm-season grasses (NWSG) can produce high quality forage and high rates of gain for beef cattle. However, little data is available on how NWSG affect the productivity of cow-calf operations on a farm scale. Therefore, we implemented an experiment at three sites, Booneville, AR, Linneus, MO and Louisville, TN, with cow-calf pairs (mature cows over ≥ 3 years old, spring calving). We evaluated two forage systems that mix either a drought or drought/flood tolerant native C4 species [big bluestem (BB) blend or eastern gamagrass (EG)] with a cool-season perennial, tall fescue (TF), and compared them to the most frequently used forage system within the Fescue Belt region, one that relies on TF only. The TN study site contains EG, with big bluestem at the MO site, and both big bluestem and EG at the AR site. Cattle (n = 12 pairs per experimental unit) were weighed yearly before initial grazing and again after final removal. Forage samples (n = 15) were collected at the beginning of grazing and once every twenty-eight days during the grazing season, and finally, at the conclusion of grazing. Harvested forages were tested for forage nutritive content (CP, NDF, ADF) using NIRS. Hay produced per forage system was documented by counting bales and weights of subsamples. The AR site was not able to participate in the first year of the study. Overall, there were no statistical differences between treatments in the first grazing season for either cattle or forage measures. However, cattle spent less time on NWSG in 2021 at the TN site to enable renovation of EG to be completed. Also, TF stands had a significant proportion of volunteer warm-season grasses within the pastures. Data from the second year of the study are currently under analysis

Muscle Glycogen Depletion Following 75-km of Cycling Is Not Linked to Increased Muscle IL-6, IL-8, and MCP-1 mRNA Expression and Protein Content

The cytokine response to heavy exertion varies widely for unknown reasons, and this study evaluated the relative importance of glycogen depletion, muscle damage, and stress hormone changes on blood and muscle cytokine measures. Cyclists (N=20) participated in a 75-km cycling time trial (168±26.0 min), with blood and vastus lateralis muscle samples collected before and after. Muscle glycogen decreased 77.2±17.4%, muscle IL-6, IL-8, and MCP-1 mRNA increased 18.5±2.8-, 45.3±7.8-, and 8.25±1.75-fold, and muscle IL-6, IL-8, and MCP-1 protein increased 70.5±14.1%, 347±68.1%, and 148±21.3%, respectively (all, P<0.001). Serum myoglobin and cortisol increased 32.1±3.3 to 242±48.3 mg/mL, and 295±27.6 to 784±63.5 nmol/L, respectively (both P<0.001). Plasma IL-6, IL-8, and MCP-1 increased 0.42±0.07 to 18.5±3.8, 4.07±0.37 to 17.0±1.8, and 96.5±3.7 to 240±21.6 pg/mL, respectively (all P<0.001). Increases in muscle IL-6, IL-8, and MCP-1 mRNA were unrelated to any of the outcome measures. Muscle glycogen depletion was related to change in plasma IL-6 (r=0.462, P=0.040), with change in myoglobin related to plasma IL-8 (r=0.582, P=0.007) and plasma MCP-1 (r=0.457, P=0.043), and muscle MCP-1 protein (r=0.588, P=0.017); cortisol was related to plasma IL-8 (r=0.613, P=0.004), muscle IL-8 protein (r=0.681, P=0.004), and plasma MCP-1 (r=0.442, P=0.050). In summary, this study showed that muscle IL-6, IL-8, and MCP-1 mRNA expression after 75-km cycling was unrelated to glycogen depletion and muscle damage, with change in muscle glycogen related to plasma IL-6, and changes in serum myoglobin and cortisol related to the chemotactic cytokines IL-8 and MCP-1

A single blind randomized controlled trial of cognitive behavioural therapy in a help-seeking population with an At Risk Mental State for psychosis: the Dutch Early Detection and Intervention Evaluation (EDIE-NL) trial

BACKGROUND: Psychotic disorders are a serious mental health problem. Intervention before the onset of psychosis might result in delaying the onset, reducing the impact or even preventing the first episode of psychosis. This study explores the effectiveness of cognitive behavioural therapy (CBT) in targeting cognitive biases that are involved in the formation of delusions in persons with an ultra-high risk for developing psychosis. A single blind randomised controlled trial compares CBT with treatment as usual in preventing or delaying the onset of psychosis. METHOD/DESIGN: All help seeking patients aged 14 to 35 years referred to the mental health services in three regions in the Netherlands are pre-screened with the Prodromal Questionnaire during a period of two years. Patients with a score of 18 or more on the sub-clinical positive symptoms items (45 items in total) will be assessed with the Comprehensive Assessment of At Risk Mental State (CAARMS). In a different pathway to care model all referrals from the mental health services in Amsterdam to the specialized psychosis clinic of the Academic Medical Centre in Amsterdam are also assessed with the CAARMS. The primary outcome is the transition rate to psychosis according to the CAARMS-criteria. Group differences will be analysed with chi-square tests and survival analyses. DISCUSSION: CBT is a highly tolerated treatment. The psycho-educational CBT approach may prove to be a successful strategy since most people with an At Risk Mental State (ARMS) are distressed by odd disturbing experiences. Giving explanations for and normalising these experiences may reduce the arousal (distress) and therefore may prevent people from developing a catastrophic delusional explanation for their odd experiences and thus prevent them from developing psychosis. Screening the entire help-seeking population referred to community mental health services with a two-stage strategy, as compared with traditional referral to a specialist clinical psychosis centre, might detect more ultra-high-risk (UHR) patients. This type of screening could be implemented in mental health care as routine screening. The trial is registered at Current Controlled trials as trial number ISRCTN21353122

Prognostic Value of Subclinical Coronary Artery Disease in Atrial Fibrillation Patients Identified by Coronary Computed Tomography Angiography

Identifying coronary artery disease (CAD) in atrial fibrillation (AF) patients improves risk stratification and defines clinical management. However, the value of screening for subclinical CAD with cardiac CT in AF patients is unknown. Between 2011 and 2015, 94 consecutive patients without known or suspected CAD (66 (57–73) years, 68% male), who were referred for AF evaluation, under