A preliminary study on metabolome profiles of buffalo milk and corresponding mozzarella cheese: Safeguarding the authenticity and traceability of protected status buffalo dairy products

The aim of this study is to combine advanced GC-MS and metabolite identification in a robust and repeatable technology platform to characterize the metabolome of buffalo milk and mozzarella cheese. The study utilized eleven dairies located in a protected designation of origin (PDO) region and nine dairies located in non-PDO region in Italy. Samples of raw milk (100 mL) and mozzarella cheese (100 g) were obtained from each dairy. A total of 185 metabolites were consistently detected in both milk and mozzarella cheese. The PLS-DA score plots clearly differentiated PDO and non-PDO milk and mozzarella samples. For milk samples, it was possible to divide metabolites into two classes according to region: those with lower concentrations in PDO samples (galactopyranoside, hydroxybuthyric acid, allose, citric acid) and those with lower concentrations in non-PDO samples (talopyranose, pantothenic acid, mannobiose, etc.,). The same was observed for mozzarella samples with the proportion of some metabolites (talopyranose, 2, 3-dihydroxypropyl icosanoate, etc.,) higher in PDO samples while others (tagatose, lactic acid dimer, ribitol, etc.,) higher in non-PDO samples. The findings establish the utility of GC-MS together with mass spectral libraries as a powerful technology platform to determine the authenticity, and create market protection, for “Mozzarella di Bufala Campana.

Corpus luteum color doppler ultrasound and pregnancy outcome in buffalo during the transitional period

This study evaluated corpus luteum (CL) development in buffaloes out of breeding season and assessed an early pregnancy diagnosis. Mediterranean buffaloes (n = 29) were synchronized and artificially inseminated. CL B-mode/color Doppler ultrasonography examinations were performed daily from Days 5 to 10 post-synchronization, recording CL dimensions and blood flow parameters. Blood samples were collected on the same days for the progesterone (P4 ) assay. Data were grouped into pregnant or nonpregnant and retrospectively analyzed. The total pregnancy rate was 50.0% (13/26) on Day 45. A significant difference between CL average area in pregnant and nonpregnant buffaloes was recorded only on Day 10. Pregnant buffaloes showed a significantly higher mean P4 concentration and higher mean time average medium velocity (TAMV) values from Day 5 to Day 10 compared to nonpregnant buffaloes. Linear regression analysis showed a significant relationship between P4 levels and TAMV. Multiple logistic regression highlighted a significant influence of TAMV on pregnancy outcome, particularly on Day 8. This is probably due to the strong relationship between TAMV and P4 production. Both TAMV and P4 could be used to predict pregnancy starting on Day 6, although a more reliable result was obtained at Day 10. Thus, the period between Days 5 and 10 is critical for CL development during the transitional period in buffalo

A monoinstitutional experience and literature review

Background We evaluated the frequency of incidental papillary thyroid microcarcinomas (mPTC) in thyroidectomies performed for benign diseases, to better characterize this nosologic entity and to assess the best treatment

Physical activity in elderly kidney transplant patients with multiple renal arteries

Introduction: Kidney transplantation (KT) is the gold standard for treatment of patients with end- stage-renal disease. To expand the donor reserve, it is necessary to use marginal/suboptimal kidneys. Methods: We retrospectively evaluated the short/long-term outcome of 34 KT elderly patients who received allografts with vascular abnormalities (MRA group), in comparison with 34 KT patients who received a kidney with a single renal artery (SRA group) pair-matched by age, length of time on dialysis, comorbidity and donor age. Results: All participants completed the International Physical Activity Questionnaire at KT, and then 4, 8, and 12 weeks after transplantation. Our data indicate that kidney with vascular anatomical variants may be successfully transplanted, since the overall rate of surgical complications was 20.6% in the SRA group and 17.6% in the MRA group and that the 5-year survival rate after KT was 100% in both groups. Conclusions: The data also underline that individualized physical activity programs induced similar excellent results in both groups, improving physical capacities, arterial pressure, lipid metabolism, insulin sensitivity, quality of life and physical and mental status

Colorectal cancer after bariatric surgery (Cric-Abs 2020): Sicob (Italian society of obesity surgery) endorsed national survey

Background The published colorectal cancer (CRC) outcomes after bariatric surgery (BS) are conflicting, with some anecdotal studies reporting increased risks. The present nationwide survey CRIC-ABS 2020 (Colo-Rectal Cancer Incidence-After Bariatric Surgery-2020), endorsed by the Italian Society of Obesity Surgery (SICOB), aims to report its incidence in Italy after BS, comparing the two commonest laparoscopic procedures-Sleeve Gastrectomy (SG) and Roux-en-Y gastric bypass (GBP). Methods Two online questionnaires-first having 11 questions on SG/GBP frequency with a follow-up of 5-10 years, and the second containing 15 questions on CRC incidence and management, were administered to 53 referral bariatric, high volume centers. A standardized incidence ratio (SIR-a ratio of the observed number of cases to the expected number) with 95% confidence intervals (CI) was calculated along with CRC incidence risk computation for baseline characteristics. Results Data for 20,571 patients from 34 (63%) centers between 2010 and 2015 were collected, of which 14,431 had SG (70%) and 6140 GBP (30%). 22 patients (0.10%, mean age = 53 +/- 12 years, 13 males), SG: 12 and GBP: 10, developed CRC after 4.3 +/- 2.3 years. Overall incidence was higher among males for both groups (SG: 0.15% vs 0.05%; GBP: 0.35% vs 0.09%) and the GBP cohort having slightly older patients. The right colon was most affected (n = 13) and SIR categorized/sex had fewer values < 1, except for GBP males (SIR = 1.07). Conclusion Low CRC incidence after BS at 10 years (0.10%), and no difference between procedures was seen, suggesting that BS does not trigger the neoplasm development

Lo stupore della forma. Ventuno domande a Renato Rizzi

Review of the book Edit by: Claudia SansòTitle: Ventuno domande a Renato RizziSeries: Saper credere in architetturaLanguage: ItalianPublisher: CLEANCharacteristic: format 10,1 x 14,4cm, 63 pages, colours ISBN: 978-88-8497-740-3Year: 2020Recensione al testo Titolo: Ventuno domande a Renato RizziA cura di: Claudia SansòCollana: Saper credere in architetturaLingua del testo: ItalianoEditore: CLEANCaratteristiche: formato 10,1 x 14,4cm, 63 pagine, brossura, a coloriISBN: 978-88-8497-740-3Anno: 202

Per una nuova governance della mobilità sostenibile. L'uso collettivo dell'auto. La scommessa del carpooling

Il lavoro di ricerca si è focalizzato sul tema della Mobilità sostenibile, con particolare attenzione al funzionamento dei sistemi di mobilità nei centri urbani. In particolare, insieme ad un’analisi del quadro normativo comunitario e nazionale del settore, si è data particolare attenzione al tema della partecipazione attiva dei cittadini, attraverso la promozione di un uso intelligente dell’auto privata, aumentando il numero di occupanti per auto e combinando percorsi comuni. E’ il cosiddetto “carpooling”, già previsto dal Decreto del 27/3/1998 sulla “Mobilità sostenibile nelle aree urbane” che all’art. 4 recita : “I comuni …incentivano associazioni o imprese ad organizzare servizi di uso collettivo ottimale delle autovetture , nonché a promuovere e sostenere forme di multiproprietà delle autovetture destinate ad essere utilizzate da più persone, dietro pagamento di una quota proporzionale al tempo d’uso ed ai chilometri percorsi” e che finalmente comincia a diffondersi anche in Italia . Il lavoro di ricerca, dopo aver approfondito alcune ormai consolidate esperienze internazionali , esamina le “buone prassi” che si stanno diffondendo nel nostro paese anche grazie alla diffusione di siti web specializzati e al ruolo attivo di associazioni di cittadini, enti locali e aziende. Dal “carpooling scolastico” delle mamme che si associano per accompagnare il loro figli a scuola, al sito di “Autostrade per l’Italia” che ogni giorno offre quasi 2000 opportunità di “viaggio condiviso” e corsie preferenziali e sconti sul pedaggio per il pendolari in carpooling sull’Autostrada dei Laghi(A8-A9, da Como e Varese verso Milano e viceversa) , passando per le ordinanze di molti sindaci che consentono alle auto con almeno tre passeggeri a bordo l’accesso ai centri urbani anche nei giorni di divieto per sforamento dei limiti di inquinamento atmosferico. Sono tutti esempi di una nuova governance per la mobilità sostenibile che può partire anche “dal basso”

Variazione sulla casa del Poeta Tragico a partire da LC

Osservando la famosa foto di Giorgio Sommer della Casa del Poeta Tragico vien subito voglia di completarla con la ipotesi di ricostruzione di Sir William Gell peraltro molto simile alla ricostruzione parziale realizzata in situ o a quella proposta nel Crystal Palace per l’Esposizione Universale del 1854 a Londra. Osservando invece lo schizzo ‘fulminante’ del giovane Corbù tratto dal Voyage d’Orient che ne coglie, con essenzialità, la struttura spaziale vien voglia riassumerla come traccia, ichnos, per una reinterpretazione/variazione ammissibile. L’atrium diviene il soggiorno semi pubblico in relazione allo studio misurato dal suo patio riservato, il tablinium la stanza da pranzo passante – separata dalle fauces ma connessa alla cucina e ai servizi – rivolta al peristilium su cui si rivolge, in luogo del triclinium, la camera dal letto per il poeta tragico. Per farlo proporzioni, quadrati, rettangoli aurei corrispondenze e tracciati regolatori ma soprattutto una verifica in sezione delle gerarchie tra gli spazi e delle ombre anch’esse tragiche tra profondi tagli di luce