sj-docx-1-npx-10.1177_1934578X221141163 - Supplemental material for New Phenylethanoid and Other Compounds From <i>Passiflora foetida</i> L., With Their Nitric Oxide Inhibitory Activities

Supplemental material, sj-docx-1-npx-10.1177_1934578X221141163 for New Phenylethanoid and Other Compounds From Passiflora foetida L., With Their Nitric Oxide Inhibitory Activities by Nguyen Van Linh, Nguyen Trung Tuong, Pham Xuan Phong and Do Thi Trang, Nguyen Xuan Nhiem, Do Hoai An, Bui Huu Tai in Natural Product Communications</p

sj-docx-1-npx-10.1177_1934578X241226825 - Supplemental material for Neolignan Glycoside and Other Constituents From the Leaves of <i>Ligustrum sinense</i> and Their Anti-Inflammatory Activity

Supplemental material, sj-docx-1-npx-10.1177_1934578X241226825 for Neolignan Glycoside and Other Constituents From the Leaves of Ligustrum sinense and Their Anti-Inflammatory Activity by Nong Thi Anh Thu, Lo Huyen Linh, Dao Anh Hoang, Nguyen Tu Oanh, Vu Mai Thao, Nguyen Thi Minh Hang and Nguyen Xuan Nhiem in Natural Product Communications</p

Achyranbidens A–C: three new compounds from <i>Achyranthes bidentata</i> Blume

Phytochemical study on the roots of Achyranthes bidentata Blume led to the isolation of sixteen compounds including three new ones (1–3). Their chemical structures were determined as oleanolic acid 28-O-β-D-glucopyranoside-3-O-[β-D-glucopyranosyl-(1→3)-β-D-galactopyranoside) (1), methyl (8Z,11Z)-5,6,7-trihydroxytetradeca-8,11-dienoate (2), methyl (6E,11Z)-5,8,9-trihydroxytetradeca-6,11-dienoate (3), fulgidic acid (4), (9E,11E)-13-oxooctadeca-9,11-dienoic acid (5), (9Z,11E,15Z)-13-hydroxyoctadeca-9,11,15-trienoic acid (6), oleanolic acid 28-O-β-D-glucopyranoside-3-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucuronopyranoside (7), oleanolic acid 28-O-β-D-glucopyranoside-3-O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→3)]-β-D-glucuronopyranoside (8), oleanolic acid 3-O-β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→3)]-β-D-glucuronopyranoside (9), oleanolic acid 3-O-α-L-rhamnopyranosyl-(1→3)-β-D-glucuronopyranoside (10), blumenol C glucoside (11), citroside A (12), 6S,9S-roseoside (13), ginsenoside Rg1 (14), 20-hydroxyecdysone (15), and benzyl α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (16) by spectroscopic analysis. Compounds 1, 7 and 11–16 inhibited NO production in LPS-activated RAW264.7 cells with IC50 values in the range from 28.03 to 54.23 µM (positive control, L-NMMA: IC50 = 35.52 µM). Compounds 14 and 15 showed anti α-glucosidase activity with IC50 values of 176.24 and 156.92 µM, respectively, compared with the positive control, acarbose, IC50 = 160.99 μM.</p

Anti-inflammatory Asterosaponins from the Starfish <i>Astropecten monacanthus</i>

Four new asterosaponins, astrosteriosides A–D (<b>1</b>–<b>3</b> and <b>5</b>), and two known compounds, psilasteroside (<b>4</b>) and marthasteroside B (<b>6</b>), were isolated from the MeOH extract of the edible Vietnamese starfish <i>Astropecten monacanthus</i>. Their structures were elucidated by chemical and spectroscopic methods including FTICRMS and 1D and 2D NMR experiments. The effects of the extracts and isolated compounds on pro-inflammatory cytokines were evaluated by measuring the production of IL-12 p40, IL-6, and TNF-α in LPS-stimulated bone marrow-derived dendritic cells. Compounds <b>1</b>, <b>5</b>, and <b>6</b> exhibited potent anti-inflammatory activity comparable to that of the positive control. Further studies are required to confirm efficacy <i>in vivo</i> and the mechanism of effects. Such potent anti-inflammatory activities render compounds <b>1</b>, <b>5</b>, and <b>6</b> important materials for further applications including complementary inflammation remedies and/or functional foods and nutraceuticals

Neuraminidase inhibitory activity by compounds isolated from aerial parts of <i>Rhinacanthus nasutus</i>

<p><i>Rhinacanthus nasutus</i> (L.) Kurz (Acanthaceae) is known as traditional medicine for the treatment of various diseases, such as cancer, fungal infections, herpes virus infections and several types of skin diseases in South-East Asian countries. In this study, eight compounds <b>1–8</b> were isolated from the aerial parts of <i>R. nasutus</i>. The structures of compounds were determined by the spectroscopic methods, including 1D and 2D NMR. The isolated compounds were evaluated for neuraminidase inhibitory activity. Several lignans, 2,3-bis[(4-hydroxy-3,5-dimethoxyphenyl)methyl]-1,4-butanediol (<b>5</b>) and 8,8′-bisdihydrosiringenin glucoside (<b>6</b>), significantly inhibited neuraminidase activity, which was comparable to the positive controls, mangiferin and oseltamivir. In addition, a structure-based virtual screening against neuraminidase using bioactive components was demonstrated.</p

sj-docx-1-npx-10.1177_1934578X221134882 - Supplemental material for Sarcokontums A and B, Two New 13,27-Cyclo-Pentacyclic Triterpenes from the Stems and Leaves of <b><i>Sarcosperma kontumense</i></b> Gagnep. ex Aubrév

Supplemental material, sj-docx-1-npx-10.1177_1934578X221134882 for Sarcokontums A and B, Two New 13,27-Cyclo-Pentacyclic Triterpenes from the Stems and Leaves of Sarcosperma kontumense Gagnep. ex Aubrév by Nguyen Quoc Vuong, Vu Van Chien, Nguyen Thi Hue, Pham Thi Hang, Nguyen Hoang Nam, Nguyen Le Tuan, Pham Van Cuong, Duong Thi Hai Yen, Nguyen Xuan Nhiem, Bui Huu Tai and Phan Van Kiem in Natural Product Communications</p

A new naphthoquinone analogue and antiviral constituents from the root of <i>Rhinacanthus nasutus</i>

<p><i>Rhinacanthus nasutus</i> (L.) Kurz (Acanthaceae) is known as traditional medicine for the treatment of fungal and herpes virus infections. A new naphthoquinone racemate, rhinacasutone (<b>1</b>) together with seven known compounds, rhinacanthone (<b>2</b>), rhinacanthins C, D, N, Q, and E (<b>3</b>–<b>7</b>), and heliobuphthalmin (<b>8</b>) were isolated from root of <i>R. nasutus.</i> Their structures were determined on the basis of extensive spectroscopic methods, including 1D-, 2D-NMR and MS data. All the isolated compounds were tested for their antiviral activities against PR8, HRV1B, and CVB3-infected vero cells. Compounds <b>3</b>–<b>6</b> exhibited significant antiviral activities with the IC₅₀ value ranging from 0.03 to 23.7 μM in all three infections.</p

DataSheet1_Discovery of a novel natural compound, vitekwangin B, with ANO1 protein reduction properties and anticancer potential.pdf

Background: Prostate cancer and non-small cell lung cancer (NSCLC) present significant challenges in the development of effective therapeutic strategies. Hormone therapies for prostate cancer target androgen receptors and prostate-specific antigen markers. However, treatment options for prostatic small-cell neuroendocrine carcinoma are limited. NSCLC, on the other hand, is primarily treated with epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors but exhibits resistance. This study explored a novel therapeutic approach by investigating the potential anticancer properties of vitekwangin B, a natural compound derived from Vitex trifolia.Methods: Vitekwangin B was chromatographically isolated from the fruits of V. trifolia. ANO1 protein levels in prostate cancer and NSCLC cells were verified and evaluated again after vitekwangin B treatment.Results: Vitekwangin B did not inhibit anoctamin1 (ANO1) channel function but significantly reduced ANO1 protein levels. These results demonstrate that vitekwangin B effectively inhibited cancer cell viability and induced apoptosis in prostate cancer and NSCLC cells. Moreover, it exhibited minimal toxicity to liver cells and did not affect hERG channel activity, making it a promising candidate for further development as an anticancer drug.Conclusion: Vitekwangin B may offer a new direction for cancer therapy by targeting ANO1 protein, potentially improving treatment outcomes in patients with prostate cancer and NSCLC. Further research is needed to explore its full potential and overcome existing drug resistance challenges.</p

Macrocyclic <i>bis</i>-quinolizidine alkaloids from <i>Xestospongia muta</i>

<p>A new stereoisomer <i>Meso</i>-araguspongine C together with nine reported macrocyclic <i>bis</i>-quinolizidine alkaloids araguspongines A, C, E, L, N−P, petrosin, and petrosin A were isolated from marine sponge <i>Xestospongia muta</i>. Stereochemistry of <i>meso</i>-araguspongine C (<b>2</b>) and araguspongines N-P (<b>3</b>−<b>5</b>) were established by their NMR data and conformational analyses. Both araguspongine C (<b>1</b>) and <i>meso</i>-araguspongine C (<b>2</b>) exhibited great cytotoxic activity towards HepG-2, HL-60, LU-1, MCF-7, and SK-Mel-2 human cancer cells (IC₅₀ in the range of 0.43–1.02 μM). At a concentration of 20 μM, isolated compounds (<b>1</b>−<b>10</b>) also showed modest inhibitory effects (from 7.6 to 40.8%) on the NO production in LPS activated RAW264.7 macrophages.</p

Anti-inflammatory coumarins from <i>Paramignya trimera</i>

<p><b>Context:</b><i>Paramignya trimera</i> (Oliv.) Burkill (Rutaceae) has been used to treat liver diseases and cancer. However, the anti-inflammatory effects of this medicinal plant and its components have not been elucidated.</p> <p><b>Objective:</b> This study investigated chemical constituents of the <i>P. trimera</i> stems and evaluated anti-inflammatory effects of isolated compounds.</p> <p><b>Materials and methods:</b> Cytotoxicity of isolated compounds (5–40 μM) toward BV2 cells was tested using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) for 24 h. Inhibitory effects of isolated compounds (5-40 μM) on nitrite and PGE₂ concentrations were determined using Griess reaction and PGE₂ ELISA kit, respectively (pretreated with the compounds for 3 h and then stimulated for 18 h with LPS). Inhibitory effects of compounds (5-40 μM) on iNOS and COX-2 protein expression were evaluated by Western blot analysis (pretreated with the compounds for 3 h and then stimulated for 24 h with LPS).</p> <p><b>Results:</b> Seven coumarins were isolated and identified as: ostruthin (<b>1</b>), ninhvanin (<b>2</b>), 8-geranyl-7-hydroxycoumarin (<b>3</b>), 6-(6′,7′-dihydroxy-3′,7′-dimethylocta-2′-enyl)-7-hydroxycoumarin (<b>4</b>), 6-(7-hydroperoxy-3,7-dimethylocta-2,5-dienyl)-7-hydroxycoumarin (<b>5</b>), 6-(2-hydroxyethyl)-2,2-dimethyl-2<i>H</i>-1-benzopyran (<b>6</b>), and luvangetin (<b>7</b>). Compounds <b>1</b>–<b>4</b> and <b>7</b> inhibited NO and PGE₂ production in LPS-stimulated BV2 cells, with IC₅₀ values ranging from 9.8 to 46.8 and from 9.4 to 52.8 μM, respectively. Ostruthin (<b>1</b>) and ninhvanin (<b>2</b>) were shown to suppress LPS-induced iNOS and COX-2 protein expression.</p> <p><b>Discussion and conclusion:</b> The present study provides a scientific rationale for the use of <i>P. trimera</i> in the prevention and treatment of neuroinflammatory diseases. Ostruthin and ninhvanin might have potential therapeutic effects and should be considered for further development as new anti-neuroinflammatory agents.</p