HPLC-DAD Based Polyphenolic Profiling and Evaluation of Pharmacological Attributes of Putranjiva roxburghii Wall.

The current study was intended to explore the phytochemical profiling and therapeutic activities of Putranjiva roxburghii Wall. Crude extracts of different plant parts were subjected to the determination of antioxidant, antimicrobial, antidiabetic, cytotoxic, and protein kinase inhibitory potential by using solvents of varying polarity ranges. Maximum phenolic content was notified in distilled water extracts of the stem (DW-S) and leaf (DW-L) while the highest flavonoid content was obtained in ethyl acetate leaf (EA-L) extract. HPLC-DAD analysis confirmed the presence of various polyphenols, quantified in the range of 0.02 ± 0.36 to 2.05 ± 0.18 μg/mg extract. Maximum DPPH scavenging activity was expressed by methanolic extract of the stem (MeOH-S). The highest antioxidant capacity and reducing power was shown by MeOH-S and leaf methanolic extract (MeOH-L), respectively. Proficient antibacterial activity was shown by EA-L extract against Bacillus subtilis and Escherichia coli. Remarkable α-amylase and α-glucosidase inhibition potential was expressed by ethyl acetate fruit (EA-F) and n-Hexane leaf (nH-L) extracts, respectively. In case of brine shrimp lethality assay, 41.67% of the extracts (LC50 < 50 µg/mL) were considered as extremely cytotoxic. The test extracts also showed mild antifungal and protein kinase inhibition activities. The present study explores the therapeutic potential of P. roxburghii and calls for subsequent studies to isolate new bioactive leads through bioactivity-guided isolation

A Revision and Pectoral Guide to the Checklist of Birds of Salt Range

Birds as major important component of biodiversity provide a vital aspect of nature’s beauty and completes important ecological role in ecosystem. Current study was designed to gather baseline information about birds of salt range which tends to be modifying with the passage of time. The area was surveyed, pictures of birds were taken and after identification a checklist was formulated and changes in biodiversity was notified

A Study on Avifauna Present in Different Zones of Chitral Districts

Chitral is a unique division ofPakistanin North western extremes of the country lies in the Hindukush range. Because of its isolation from other adjacent areas, its ecology is least disturbed and also lacks the proper scientific checklist of its flora and fauna. This study is designed to explore its various regions to gather the data of both Passerines and Non- passerines currently present in the region. Several visits will be made, birds will be searched, pictures will be taken and after identification checklist will be formulated and its comparison with existing data will be reported

An Attempt to Update a Checklist and Some Other Aspects of Murree Hills’ Avifauna

Birds are vital component of biodiversity as they are playing a significant role in an ecosystem. Increasing human interference might have affected previously reported diversity of birds. This study was designed to collect information about birds check list data of Murree hills. Area was surveyed at different times of day and different months of season. During visits, pictures of birds were taken and identification as well as preparation of list was carried out. Comparison of new checklist with previous literature was done in order to get revised checklist of existing species

Rescue and Rehabilitation of an Indian Rock Python (Python Molurus): First Case Study from Pakistan

Pythons are facing the threat of extinction due to human annihilation and interference in natural habitats of pythons. Indian rock python (Python molurus) has been stated as Lower Risk/Near Threatened by International Union for the Conservation of Nature (IUCN). Therefore, there is an intense need to change the perception of people and encourage them to coincide with this big snake. Current study involved the rescue and rehabilitation of an Indian rock python (P. molurus) spotted at the shrine of Baba Shah Jeevan, Rawalpindi, Pakistan. Python was grasped by skilled snake catchers and taken to the wildlife sanctuary in Balkasar Research Complex, Chakwal, Pakistan for the purpose of conservation. The python was kept in cage designed for reptiles (especially for snakes) having proper soil bed and shelter. Proper hygienic condition is maintained in the cage with climbing structures for the python and an adult chicken is feed to it every week. After rescuing, the python was force-feed, however the natural feeding behavior of constriction and killing of prey was resumed by it after few weeks. Rescue, rehabilitation and release of pythons create a coexisting environment in between pythons and human being instead of python-human conflict, ultimately decreasing the risk of population decline of large snakes

Phytochemical Analysis and Antioxidant Potential of Ficus Benghalensis L.

Genus Ficus belongs to family Moraceae having 40 genera and over 1000 species worldwide. Different methods have been used for phytochemical screening of medicinal plants like total phenolics content (TPC) and total flavonoids content (TFC) assays to quantify phenolics and flavonoids. The phytochemical analysis exhibited highest total phenolics content in M extract of stem and total flavonoids content in ethyl acetate (EA) extract of leaves i.e. 61.2±1.3 µg GAE/mg extract and 25.1±0.9 µg QE/mg extract respectively. Total reduction power and total antioxidant capacity were maximum in the M extract of stem i.e. 243.89±1.6 µg AAE/mg extract and 127.08±2.7 µg AAE/mg extract respectively

Revealing the Phytochemical Profile and Antioxidant Potential of a Medicinal Folklore: Bombax Ceiba L.

Current study intends to investigate the extraction yield, phytochemical analysis and antioxidant potential of selected plant by executing a wide range of in vitro assays. This was done to suggest the most proficient solvent system and plant part for isolation and purification of probable bioactive leads. The colorimetric estimation of total phenolic, flavonoid contents, total antioxidant capacity, total reducing power and free radical scavenging activity was done. The specific polyphenols were quantified by RP-HPLC analysis. The present findings proved acetone and methanol (AC:M) extract of root bark part as an enriched source of antioxidant phytochemicals. The highest amount of rutin was detected in methanol (M) extract of root bark i.e. 0.70 μg/mg DW followed by methanol (M) extract (0.52 μg/mg DW) of leaf. Bombax ceiba L. was found to be a reservoir of phytochemicals possessing antioxidant potential

HPLC-DAD based polyphenolic profiling and evaluation of pharmacological attributes of Putranjiva roxburghii Wall.

The current study was intended to explore the phytochemical profiling and therapeutic activities of Putranjiva roxburghii Wall. Crude extracts of different plant parts were subjected to the determination of antioxidant, antimicrobial, antidiabetic, cytotoxic, and protein kinase inhibitory potential by using solvents of varying polarity ranges. Maximum phenolic content was notified in distilled water extracts of the stem (DW-S) and leaf (DW-L) while the highest flavonoid content was obtained in ethyl acetate leaf (EA-L) extract. HPLC-DAD analysis confirmed the presence of various polyphenols, quantified in the range of 0.02 ± 0.36 to 2.05 ± 0.18 μg/mg extract. Maximum DPPH scavenging activity was expressed by methanolic extract of the stem (MeOH-S). The highest antioxidant capacity and reducing power was shown by MeOH-S and leaf methanolic extract (MeOH-L), respectively. Proficient antibacterial activity was shown by EA-L extract against Bacillus subtilis and Escherichia coli. Remarkable α-amylase and α-glucosidase inhibition potential was expressed by ethyl acetate fruit (EA-F) and n-Hexane leaf (nH-L) extracts, respectively. In case of brine shrimp lethality assay, 41.67% of the extracts (LC(50) < 50 µg/mL) were considered as extremely cytotoxic. The test extracts also showed mild antifungal and protein kinase inhibition activities. The present study explores the therapeutic potential of P. roxburghii and calls for subsequent studies to isolate new bioactive leads through bioactivity-guided isolation