Characterization and evaluation ethyl acetate extract of melochia corchorifolia leaf- anticancer antibiological and molecular docking studies on breast cancer estrogen receptor

The present work focused on Phytochemical screening, characterization, anticancer activity and antibiological activity of ethyl acetate extract of Melochia corchorifolia leaves followed by molecular docking studies have been carried out. The plant leaves have been collected, weighed, and extracted with the soxhlet apparatus by using ethyl acetate solvent and then extracted are subjected to phytochemical screening.  Antibiological activity of plant leaves ethyl acetate extract has been tested against six bacterial and two fungal strains using agar well diffusion methodology. The characterization of phytoconstituents compounds has been carried out using various spectroscopy method such as GC-MS (Gas chromatography Mass spectroscopy), UV-visible and Fourier-transform infrared. Auto dock tool (4.2.0) is used for molecular docking studies. The phytochemical analysis of Melochia corchorifolia ethyl acetate leaves, reveals the existence of carbohydrates, glycosides, triterpenes flavonoids and alkaloids. Antimicrobial activity is effective against gram-positive bacterial strains namely Staphylococcus aureus (17 mm), Bacillus subtilis (16 mm), the gram-negative bacterial strains namely Salmonella typhi (15 mm) and E. coli (14 mm). Moreover, the extract is also found to be effective against Aspergillus Niger (18 mm) fungal species. The GC-MS and FT-IR analysis show bioactive compounds and their functional groups. UV-VIS analysis results reveal that the presence of phytoconstituents derivatives in the range between 206-350 nm. The cytotoxicity activity for the MCF-7 cell line shows that the drug efficacy IC50 value is 148.836 (μg/mL). Further, the predicted bioactive compounds are docked with the cancer estrogen protein receptor (PDB ID: 3s7s) with ligand martidin-15 one shows the highest binding affinity. The study reveals the potential of Melochia corchorifolia leaves ethyl acetate extract showed antibiological and anticancer activity

Совершенствование системы мотивации и стимулирования труда работников на примере ПАО "Ростелеком"

Объект ВКР - система мотивации и стимулирования персонала. Цель ВКР – изучение особенностей системы мотивации и стимулирования труда работников ПАО "Ростелеком" ,анализ применяемых методов мотивации и стимулирования и разработка рекомендаций. В результате исследования был разработан ряд мероприятий по совершенствованию системы мотивации и стимулирования персоналом.Object of WKR is a system of motivation and incentives for staff. The purpose of WKR is to study the features of the system of motivation and motivation of the work of PAO "Rostelecom" employees, analyze the methods of motivation and incentives used and develop recommendations. As a result of the research, a number of measures were developed to improve the system of motivation and incentives for staff

Role of CYP27A in cholesterol and bile acid metabolism

The CYP27A gene encodes a mitochondrial cytochrome P450 enzyme, sterol 27-hydroxylase, that is expressed in many different tissues and plays an important role in cholesterol and bile acid metabolism. In humans, CYP27A deficiency leads to cerebrotendinous xanthomatosis. To gain insight into the roles of CYP27A in the regulation of cholesterol and bile acid metabolism, cyp27A gene knockout heterozygous, homozygous, and wild-type littermate mice were studied. In contrast to homozygotes, heterozygotes had increased body weight and were mildly hypercholesterolemic, with increased numbers of lipoprotein particles in the low density lipoprotein size range. Cyp7A expression was not increased in heterozygotes but was in homozygotes, suggesting that parts of the homozygous phenotype are secondary to increased cyp7A expression and activity. Homozygotes exhibited pronounced hepatomegaly and dysregulation in hepatic cholesterol, bile acid, and fatty acid metabolism. Hepatic cholesterol synthesis and synthesis of bile acid intermediates were increased; however, side chain cleavage was impaired, leading to decreased bile salt concentrations in gallbladder bile. Expression of Na-taurocholate cotransporting polypeptide, the major sinusoidal bile salt transporter, was increased, and that of bile salt export pump, the major canalicular bile salt transporter, was decreased. Gender played a modifying role in the homozygous response to cyp27A deficiency, with females being gen

Evaluation of renal lesions and clinicopathologic correlation in rheumatoid arthritis

The most common causes of renal disease in rheumatoid arthritis (RA) are glomerulonephritis (GN), amyloidosis, tubulo-interstitial nephritis, and drug toxicity. Our aim was to evaluate the clinicopathologic correlation of renal lesions and to assess the course and prognosis of renal disease in patients with RA. We conducted a prospective observational study in all adult patients with RA between July 2010 and June 2015. The total number of patients studied was 90, with a female:male ratio of 2.3:1. Mean follow-up duration was 30 ± 6.5 months. About 54 patients (60%) were asymptomatic. The most common symptom was edema legs (30%), followed by oliguria (10%). About 18 patients (20%) presented with the nephrotic syndrome, 15 patients (16.6%) with nephritic syndrome, and 30 (33%) with asymptomatic urinary abnormalities. Chronic kidney disease (CKD) was seen in 48 of 90 patients (53%).The most common renal pathology noted was mesangioproliferative GN followed by membranous nephropathy (MN). IgM with C3 deposits was the most common immunofluorescence pattern observed. Among the patients who had glomerular diseases, complete remission was seen in nine patients, partial remission in 15, and persistent proteinuria in 14. Duration of RA and a high erythrocyte sedimentation rate correlated significantly with persistent proteinuria. Only one patient in the glomerular disease group progressed to dialysis-dependent renal failure. On followup, 11 out of 48 CKD patients showed a significant decrease in estimated glomerular filtration rate and worsened to the next stage of CKD. Renal disease in RA presents with varied renal pathology. MN was seen frequently and was not associated with gold or penicillamine usage. Relatively high incidence of CKD was noted. Hence, it is important to monitor renal function abnormalities periodically in these patients

Paraneoplastic glomerulopathies associated with hematologic malignancies

Paraneoplastic glomerulopathy (PG) includes a group of glomerular lesions that are not directly related to tumor burden, invasion, or metastasis, but induced by products from tumor cells such as hormones, growth factors, cytokines, and tumor antigens. PG was first described by Galloway in 1922, in which nephrotic syndrome (NS) secondary to Hodgkin's lymphoma was reported. They can present before, at the time of diagnosis, during remission, or at relapse of the hematological malignancies. We present three cases of paraneoplastic NS associated with hematological malignancies. Early diagnosis and treatment can alter prognosis of these patients. Paraneoplastic syndromes would remit only with proper treatment of the neoplasia