4 research outputs found
THE BROWN-CHESTED FLYCATCHER RHINOMYIAS-BRUNNEATA NEW-RECORD FOR THAILAND
Volume: 105Start Page: 75End Page: 7
World congress of soil science : frontiers of soil science : technology and the information age : abstracts
MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation
Mutations in the mitochondrial DNA (mtDNA) are responsible for several metabolic disorders, commonly involving muscle and the central nervous system
1
. Because of the critical role of mtDNA in oxidative phosphorylation, the majority of pathogenic mtDNA mutations are heteroplasmic, co-existing with wild-type molecules
1
. Using a mouse model with a heteroplasmic mtDNA mutation
2
, we tested whether mitochondrial-targeted TALENs (mitoTALENs)
3
,
4
could reduce the mutant mtDNA load in muscle and heart. AAV9-mitoTALEN was administered via intramuscular, intravenous, and intraperitoneal injections. Muscle and heart were efficiently transduced and showed a robust reduction in mutant mtDNA, which was stable over time. The molecular defect, namely a decrease in transfer RNA
Ala
levels, was restored by the treatment. These results showed that mitoTALENs, when expressed in affected tissues, could revert disease-related phenotypes in mice