Identification of Campylobacter Jejuni and Campylobacter Coli from Diarrheic Samples Using PCR

Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored.Methods: Detection was performed using diarrheal samples collected 8 from 117 children. The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method. Results: Of 117 diarrheal samples, 35 (29.9%) were found positive for 10 Campylobacter spp using PCR. Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity

Identification of Campylobacter Jejuni and Campylobacter Coli from Diarrheic Samples Using PCR

Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored.Methods: Detection was performed using diarrheal samples collected 8 from 117 children. The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method. Results: Of 117 diarrheal samples, 35 (29.9%) were found positive for 10 Campylobacter spp using PCR. Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity

EVALUATION OF PHYTOREMEDIATION POTENTIAL OF ROSEMARY PLANT IN CONTAMINATED SOILS WITH CADMIUM AND ZINC

For evaluation of Phytoremediation potential of Rosemary plant in contaminated soils with Cadmium and Zinc, Two Greenhouse pot culture experiments were conducted in Fars Education Center of Jahad-e-Agriculture- Aliabad kamin. Treatments included soil cadmium with six levels (0, 10, 20, 40, 80 and 160 ppm) and zinc with six levels (0, 25, 50, 100, 200 and 400 ppm). Each treatment was replicated three times. In this study, cadmium sulfate and zinc sulfate were used, for contaminate soil samples different amounts of salts dissolved in distilled water and sprayed on the soil. The results showed, with increasing cadmium and zinc concentrations in soil, cadmium and zinc concentration increased in root and shoots. The highest cadmium and zinc transfer factor was observed in treatment 80 and 25 ppm respectively. Maximum bio accumulation factor for cadmium and zinc obtained from 80 and 400 ppm respectively. Rosemary plant can fix the elements of cadmium and zinc and transfer it to the shoot