18 research outputs found
Central role of the exchange factor GEF-H1 in TNF-α–induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells
A soy-based phosphatidylserine/ phosphatidic acid complex (PAS) normalizes the stress reactivity of hypothalamus-pituitary-adrenal-axis in chronically stressed male subjects: a randomized, placebo-controlled study
Effects of pyrrolidine dithiocarbamate on proliferation and nuclear factor-ÎşB activity in autosomal dominant polycystic kidney disease cells
Follicular microautotransplantation in the treatment of patients with scalp scarring defects
Objective: to evaluate the efficiency of follicular microautotransplantation in the treatment of scarring alopecia.Subjects and methods. The results of surgical treatment were analyzed in 62 patients with scalp scars at various sites after injuries, burns, and surgery. Surgical treatment was performed using follicular microautotransplantation. The surgery was made as an outpatient procedure (the patients did not need hospitalization) and it involved 4 basic stages. The efficiency of the surgical treatment for scarring alopecia was evaluated, by analyzing its early and late results. Gender, age, etiology, and scar time did not affect the results of the treatment.Results. The early surgical results were assessed from indicators, such as the proportion of surviving grafts and the quality of restored hair. The late results were followed up for at least 9 months. The maximum follow-up was 2.8 years. The engraftment rate of transplanted follicles averaged 93.8%; it was 96.6% for a normotrophic scar.Conclusion. Transfer of own hair by follicular microautotransplantation is an effective surgical treatment in patients with scalp scarring defects; it yields a good cosmetic effect and substantially improves quality of life in patients with scarring alopecia
Long-term results of maintenance treatment of mitomycin C or alternating mitomycin C and bacillus Calmette–Guérin instillation therapy of patients with carcinoma in situ of the bladder: A subgroup analysis of the prospective FinnBladder 2 study with a 17-year follow-up
Comparison of stress and burnout among anesthesia and surgical residents in a tertiary care teaching hospital in North India
The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins
How individual cells respond to mechanical forces is of considerable interest to biologists as force affects many aspects of cell behavior1. Application of force on integrins triggers cytoskeletal rearrangements and growth of the associated adhesion complex, resulting in increased cellular stiffness2,3, also known as reinforcement4. While RhoA has been shown to play a role during reinforcement3, the molecular mechanisms that regulate its activity are unknown. By combining biochemical and biophysical approaches, we identified two guanine nucleotide exchange factors (GEFs), LARG and GEF-H1, as key molecules that regulate the cellular adaptation to force. We show that stimulation of integrins with tensional force triggers activation of these two GEFs and their recruitment to adhesion complexes. Surprisingly, activation of LARG and GEF-H1 involves distinct signaling pathways. Our results reveal that LARG is activated by the Src family tyrosine kinase Fyn, whereas GEF-H1 catalytic activity is enhanced by ERK downstream of a signaling cascade that includes FAK and Ras. To analyze the effect of force on RhoA activity we applied a constant force for different amounts of time on fibronectin (FN)-coated beads using a permanent magnet. Consisten