14 research outputs found
Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels
This report describes the development of endothelial cell (EC) cultivation devices with different channel widths (60 to 360 μm). Crucial features of the devices include even cell distribution along the channel, seeding reproducibility, and compatibility with microscopy and flow application. The main achievement of this work is the design of chips which allow reproducible HUVEC culture in narrow (< 400 μm) channels.</p
Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels
This report describes the development of endothelial cell (EC) cultivation devices with different channel widths (60 to 360 μm). Crucial features of the devices include even cell distribution along the channel, seeding reproducibility, and compatibility with microscopy and flow application. The main achievement of this work is the design of chips which allow reproducible HUVEC culture in narrow (< 400 μm) channels.</p
Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels
This report describes the development of endothelial cell (EC) cultivation devices with different channel widths (60 to 360 μm). Crucial features of the devices include even cell distribution along the channel, seeding reproducibility, and compatibility with microscopy and flow application. The main achievement of this work is the design of chips which allow reproducible HUVEC culture in narrow (< 400 μm) channels.</p
Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels
This report describes the development of endothelial cell (EC) cultivation devices with different channel widths (60 to 360 μm). Crucial features of the devices include even cell distribution along the channel, seeding reproducibility, and compatibility with microscopy and flow application. The main achievement of this work is the design of chips which allow reproducible HUVEC culture in narrow (< 400 μm) channels.</p
Characterization of a hydrostatically driven cell seeding procedure using polymer microspheres
This paper describes how particles are distributed upon introduction into microchannels using a seeding technique based on hydrostatic flow. The distribution of particles and/or cells in microchannels is characterized in time, and an approach for ensuring a reproducible distribution which involves closing off reservoirs at defined times is presented.</p
Endothelial cell phenotype on gelatin micropatterns compared to that in microchannels
Our long-term interest is to develop new techniques for adherent cell-based drug screening by using microtechnologies to create in vivo-like microenvironments. This paper explores the influence of surface properties on the morphology of adherent cells in the presence or absence of a microchannel.</p
Endothelial cell phenotype on gelatin micropatterns compared to that in microchannels
Our long-term interest is to develop new techniques for adherent cell-based drug screening by using microtechnologies to create in vivo-like microenvironments. This paper explores the influence of surface properties on the morphology of adherent cells in the presence or absence of a microchannel.</p
Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels
Endothelial cell phenotype on gelatin micropatterns compared to that in microchannels
Our long-term interest is to develop new techniques for adherent cell-based drug screening by using microtechnologies to create in vivo-like microenvironments. This paper explores the influence of surface properties on the morphology of adherent cells in the presence or absence of a microchannel.</p