The study of bombesin-like neuropeptides from mammals

About 20 years has elapsed since the discovery of bombesin by Erspamer and his coworkers.In this period,more than 10 peptides were isolated and characterized.These included gastrinreleasing peptide(GRP),neuromedin B(NMB)and their fragment ...Thesis--University of Tsukuba, D.Agr.(A), no. 728, 1990. 3. 2

GRIFFIN: a system for predicting GPCR–G-protein coupling selectivity using a support vector machine and a hidden Markov model

We describe a novel system, GRIFFIN (G-protein and Receptor Interaction Feature Finding INstrument), that predicts G-protein coupled receptor (GPCR) and G-protein coupling selectivity based on a support vector machine (SVM) and a hidden Markov model (HMM) with high sensitivity and specificity. Based on our assumption that whole structural segments of ligands, GPCRs and G-proteins are essential to determine GPCR and G-protein coupling, various quantitative features were selected for ligands, GPCRs and G-protein complex structures, and those parameters that are the most effective in selecting G-protein type were used as feature vectors in the SVM. The main part of GRIFFIN includes a hierarchical SVM classifier using the feature vectors, which is useful for Class A GPCRs, the major family. For the opsins and olfactory subfamilies of Class A and other minor families (Classes B, C, frizzled and smoothened), the binding G-protein is predicted with high accuracy using the HMM. Applying this system to known GPCR sequences, each binding G-protein is predicted with high sensitivity and specificity (>85% on average). GRIFFIN () is freely available and allows users to easily execute this reliable prediction of G-proteins

F-molding: A New Production Method for Largely Aspherical Mirrors of Cordierite

Cordierite is a rigid low thermal expansion coefficient ceramic equivalent to that of low thermal expansion glass but with voids due to forming with raw material powder. To overcome this problem, we propose Flexible Molding (F-molding) as our new fabrication method. With F-molding, the raw material powder is converted to liquid slurry, which is poured into a resin mold, solidified, and directly formed into near net design of a mirror. The mold prepared from F-molding is an effective tool for processing multiple volumes of identical structures such as those used in segmented mirror designs. We introduce details of our fabrication method in F-molding mirror and report its measurement results of optical performances with a 270mm diameter sample