3 research outputs found
Stability of DNA fragments of bacteriophage lambda cloned in E. coli using pBR322 vector.
Ile105Val polymorphism gene gstΟl with bronchial asthma in the uzbek population
Genes metabolizing enzymes are considered as candidates for atopy and associated diseases due to the fact that they are involved in the metabolism of mediators of allergic inflammation of leukotrienes and prostaglandins, as well as in the regulation mechanisms of oxidative stress, which is important in the pathogenesis of asthma. Analyzed the distribution of allele and genotype frequencies of polymorphic gene variant GSTΟl, in patients with bronchial asthma in a population of Uzbeks. Patients with asthma were allocated into groups according to the international classification of WHO (X revision, ICD-10) and in accordance with the diagnostic criteria (GINA, 2007). Analysis of the polymorphic gene variant GSTΟl was performed by polymerase chain reaction DNA synthesis (PCR) in thermocycler and RFLP analysis, followed by polyacrylamide gel electrophoresis. The results obtained for gene GSTΟl indicate the existence of the Association, Ρ on the one hand, the genotype lle-105/lle-105, on the other hand, carriers of allele lle-105 patients with asthma in the Uzbek population. Thus, the identification of individuals at high risk by testing the genes is essential for the proper medical and genetic counseling for subsequent timely and adequate therapy.ΠΠ΅Π½Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π±ΠΈΠΎΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΠΊΠ°ΠΊ ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΡ Π΄Π»Ρ Π°ΡΠΎΠΏΠΈΠΈ ΠΈ Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ΅ΠΌ, ΡΡΠΎ ΠΎΠ½ΠΈ ΡΡΠ°ΡΡΠ²ΡΡΡ Π² ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ΅ ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠΎΠ² Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π»Π΅ΠΉΠΊΠΎΡΡΠΈΠ΅Π½ΠΎΠ² ΠΈ ΠΏΡΠΎΡΡΠ°Π³Π»Π°Π½Π΄ΠΈΠ½ΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΎΠΊΡΠΈΠ΄Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΡΡΠ°, ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΠΠ. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΡΡΠΎΡ Π°Π»Π»Π΅Π»Π΅ΠΉ ΠΈ Π³Π΅Π½ΠΎΡΠΈΠΏΠΎΠ² ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΠ³ΠΎ Π²Π°ΡΠΈΠ°Π½ΡΠ° Π³Π΅Π½Π° GSTΟl, Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π² ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ ΡΠ·Π±Π΅ΠΊΠΎΠ². ΠΠΎΠ»ΡΠ½ΡΠ΅ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈΡΡ ΠΏΠΎ Π³ΡΡΠΏΠΏΠ°ΠΌ ΡΠΎΠ³Π»Π°ΡΠ½ΠΎ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠΉ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΠΠ (Π₯-ΠΏΠ΅ΡΠ΅ΡΠΌΠΎΡΡ, ΠΠΠ-10) ΠΈ Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΊΡΠΈΡΠ΅ΡΠΈΡΠΌΠΈ (GINA, 2007 Π³.). ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΠ³ΠΎ Π²Π°ΡΠΈΠ°Π½ΡΠ° Π³Π΅Π½Π° GSTΟl ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΡΠΈΠ½ΡΠ΅Π·Π° ΠΠΠ (ΠΠ¦Π ) Π½Π° Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΎΡΠ΅ ΠΈ ΠΠΠ Π€-Π°Π½Π°Π»ΠΈΠ·ΠΎΠΌ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·ΠΎΠΌ Π² ΠΏΠΎΠ»ΠΈΠ°ΠΊΡΠΈΠ»Π°ΠΌΠΈΠ΄Π½ΠΎΠΌ Π³Π΅Π»Π΅. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ Π³Π΅Π½Π° GSTΟl ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΡΡΠ΅ΡΡΠ²ΠΎΠ²Π°Π½ΠΈΠΈ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ, Ρ ΠΎΠ΄Π½ΠΎΠΉ ΡΡΠΎΡΠΎΠ½Ρ, Π³Π΅Π½ΠΎΡΠΈΠΏΠ° Ile-105/Ile-105, Π° Ρ Π΄ΡΡΠ³ΠΎΠΉ, Π½ΠΎΡΠΈΡΠ΅Π»ΡΡΡΠ²Π° Π°Π»Π»Π΅Π»Ρ Ile-105 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΠΠ Π² ΡΠ·Π±Π΅ΠΊΡΠΊΠΎΠΉ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π»ΠΈΡ Π³ΡΡΠΏΠΏΡ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΡΠΈΡΠΊΠ° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΈΠΌΠ΅Π΅Ρ ΠΏΡΠΈΠ½ΡΠΈΠΏΠΈΠ°Π»ΡΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π΄Π»Ρ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅Π΄ΠΈΠΊΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠ»ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π»Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅Π³ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ²ΠΎΠ΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΠΈ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ
The study of galectin-3, Ki-67, ubiquitin, HMGA-2 by polymerase chain reaction in real time (RT-PCR) in the puncture specimens of nodular goiter
Actuality.Β The differential diagnosis of follicular adenoma, follicular variant of papillary thyroid cancer and follicular thyroid cancer (TC) is one of the main topics of research. For this purpose several molecular markers were approbated, however their diagnostic effectiveness differs.
Aim.Β Quantification of candidate mRNA markers for differentiated thyroid cancer (galectin-3, Ki-67, ubiquitin, HMGA2) using polymerase chain reaction in real time (RT-PCR) of thyroid nodules pre-operative material. ToΒ evaluate the efficiency of this method for thyroid malignancy diagnostics.
Materials and methods.Β The study included 55 patients with a clinical diagnosis of nodular / multinodular goiter. AΒ quantitative analysis of mRNA of galectin-3, Ki-67, ubiquitin, HMGA2 was performed on material puncture byΒ reverse transcription and RT-PCR. The Second Derivative Maximum Method was used to analyze the results.
Results.Β The study included 46 women (83.6%) and 8 men (14.5%). The average age of the patients was 52.1 (fromΒ 23 to 82) years old. According to the results of histological examination, there were 35 (63.6%) benign tumors, 20 (36.4%) β cancer tumors (papillary carcinoma). There were no cases of follicular cancer in the histological findings. We found significant differences in the expression of mRNA of the ubiquitin gene between malignant and benign thyroid nodules.
Conclusion.Β The method of ubiquitin gene 8.24 mRNA estimation has a sensitivity of 68.4% and a specificity ofΒ 68.6% in the well-differentiated cancer diagnostics. The estimation of mRNA of gene Ki-67, galectin-3, HMGA by RT-PCR did not show itself as a reliable method for the differential diagnosis of thyroid nodules in the preoperative stage