8 research outputs found
Soil ozonation as a sustainable alternative to methyl bromide fumigation and synthetic pesticides
ABSTRACT
The goal of this dissertation was to investigate the potentials of ozone as a sustainable alternative to the soil fumigant methyl bromide and to synthetic pesticides. Three pathogens were selected for this research, given their economic importance, and the spectrum variety that they represent: Phytoparasitic nematodes, important pests that cause severe crop yield losses; Phytophthora sojae, a predominant soybean pathogen that causes root and stem rot, and pre- and post-emergence damping-off of soybean; and Fusarium oxysporum, which causes Fusarium wilt, an economically important disease in hydroponic systems.
Soil samples that were naturally infested with nematodes were treated with different levels of gaseous ozone at 21 ĂÂșC and 5 ĂÂșC. A medium level of ozonation (2.1 g O3 kg-1 for 10 min at a rate of ozonation 0.21 g O3 kg-1min-1) and low temperature (5 ĂÂșC) resulted in 96% nematode inhibition. Regression analysis showed that nematode viability was a function of the level of ozonation (P = 5.1E-07) and the soil temperature (P = 4.4E-08; Adjusted R-square = 0.65).
Assays of artificially inoculated soil samples with P. sojae were treated with different doses of gaseous ozone. This study showed that a dosage of 0.47 g O3/kg soil, totally prevented root and stem rot disease symptoms caused by P. sojae.
Samples of conidial suspensions of F. oxysporum were treated with incremental doses of ozone from either oxygen feed with high gas-phase concentration (GPC) or air feed with low GPC. Trials resulted in non-viability of the pathogen at high ozone GPC with a dose of 0.84 mg O3/L for 3 seconds. The optimal conditions for F. oxysporum treatment with ozone were high GPC (oxygen feed), and low temperature (5 ĂÂșC).
Given these promising results, and since ozone degenerates quickly to oxygen, the findings of this research clearly indicate that ozone may be an efficient and sustainable alternative to methyl bromide and to:
1. nematicides in the treatment of nematodes in the soil,
2. fungicides in the inhibition of Phytophthora diseases in the soil, and
3. fungicides in the treatment of Fusarium wilt in hydroponic nutrient solutions
Ozonation Efficacy in the Treatment of Soil-Borne Phytophthora sojae in Cultivating Soybeans
Ozonation was studied for inactivating Phytophthora sojae, a predominant soybean pathogen that causes root and stem rot, and pre-and post-emergence soybean damping-off. Typically, fungicides are used to treat soils to control the damage from P. sojae to soybean production. An environmentally friendly method of ozonation was studied for inactivating P. sojae, a model Phytophthora pathogen that affects a wide range of high-value crops. Assays of artificially inoculated soil samples with P. sojae were treated with different doses of gaseous ozone. This study showed that a dosage of 0.47 g.kg-1 O3 in the soil totally prevented root and stem-rot disease incidence by P. sojae. The findings of this research clearly indicate that ozonation is an efficient alternative to chemical fungicides in the inhibition of Phytophthora diseases in the soil, hence a balancing feedback loop reinforcing the soil system as natural capital
Soil Ozonation for Nematode Disinfestation as an Alternative to Methyl Bromide and Nematicides
Phytoparasitic nematodes are important pests that cause severe crop yield losses. In the past, methyl bromide and other proprietary nematicides have been used as management practices, but these practices are unsustainable and lead to atmospheric pollution and ozone layer destruction. Ozonation was studied as an alternative management practice since it is highly effective against microorganisms and degenerates quickly to oxygen. Soil samples that were naturally infested with nematodes were treated with different levels of gaseous ozone at 21 ÂșC and 5 ÂșC. Regression analysis results show that a medium level of ozonation (2.1 g O3 kg-1 for 15 min at a rate of ozonation 0.14 g O3 kg-1 min-1) and low temperature (5 ÂșC) resulted in 94% mean nematode inhibition
Soil ozonation as a sustainable alternative to methyl bromide fumigation and synthetic pesticides
ABSTRACT
The goal of this dissertation was to investigate the potentials of ozone as a sustainable alternative to the soil fumigant methyl bromide and to synthetic pesticides. Three pathogens were selected for this research, given their economic importance, and the spectrum variety that they represent: Phytoparasitic nematodes, important pests that cause severe crop yield losses; Phytophthora sojae, a predominant soybean pathogen that causes root and stem rot, and pre- and post-emergence damping-off of soybean; and Fusarium oxysporum, which causes Fusarium wilt, an economically important disease in hydroponic systems.
Soil samples that were naturally infested with nematodes were treated with different levels of gaseous ozone at 21 ĂÂșC and 5 ĂÂșC. A medium level of ozonation (2.1 g O3 kg-1 for 10 min at a rate of ozonation 0.21 g O3 kg-1min-1) and low temperature (5 ĂÂșC) resulted in 96% nematode inhibition. Regression analysis showed that nematode viability was a function of the level of ozonation (P = 5.1E-07) and the soil temperature (P = 4.4E-08; Adjusted R-square = 0.65).
Assays of artificially inoculated soil samples with P. sojae were treated with different doses of gaseous ozone. This study showed that a dosage of 0.47 g O3/kg soil, totally prevented root and stem rot disease symptoms caused by P. sojae.
Samples of conidial suspensions of F. oxysporum were treated with incremental doses of ozone from either oxygen feed with high gas-phase concentration (GPC) or air feed with low GPC. Trials resulted in non-viability of the pathogen at high ozone GPC with a dose of 0.84 mg O3/L for 3 seconds. The optimal conditions for F. oxysporum treatment with ozone were high GPC (oxygen feed), and low temperature (5 ĂÂșC).
Given these promising results, and since ozone degenerates quickly to oxygen, the findings of this research clearly indicate that ozone may be an efficient and sustainable alternative to methyl bromide and to:
1. nematicides in the treatment of nematodes in the soil,
2. fungicides in the inhibition of Phytophthora diseases in the soil, and
3. fungicides in the treatment of Fusarium wilt in hydroponic nutrient solutions.</p
Soil Ozonation for Nematode Disinfestation as an Alternative to Methyl Bromide and Nematicides
Phytoparasitic nematodes are important pests that cause severe crop yield losses. In the past, methyl bromide and other proprietary nematicides have been used as management practices, but these practices are unsustainable and lead to atmospheric pollution and ozone layer destruction. Ozonation was studied as an alternative management practice since it is highly effective against microorganisms and degenerates quickly to oxygen. Soil samples that were naturally infested with nematodes were treated with different levels of gaseous ozone at 21 ÂșC and 5 ÂșC. Regression analysis results show that a medium level of ozonation (2.1 g O3 kg-1 for 15 min at a rate of ozonation 0.14 g O3 kg-1 min-1) and low temperature (5 ÂșC) resulted in 94% mean nematode inhibition.This article is published as Msayleb, N, R.S. Kanwar, J. van Leeuwen, and H. Wu. âSoil Ozonation for Nematode Disinfestation as an Alternative to Methyl Bromide and Nematicidesâ Scientific Pages of Environmental Studies 1, no. 1 (2017): 11-19. Posted with permission.</p
Ozonation Efficacy in the Treatment of Soil-Borne Phytophthora sojae in Cultivating Soybeans
Ozonation was studied for inactivating Phytophthora sojae, a predominant soybean pathogen that causes root and stem rot, and pre-and post-emergence soybean damping-off. Typically, fungicides are used to treat soils to control the damage from P. sojae to soybean production. An environmentally friendly method of ozonation was studied for inactivating P. sojae, a model Phytophthora pathogen that affects a wide range of high-value crops. Assays of artificially inoculated soil samples with P. sojae were treated with different doses of gaseous ozone. This study showed that a dosage of 0.47 g.kg-1 O3 in the soil totally prevented root and stem-rot disease incidence by P. sojae. The findings of this research clearly indicate that ozonation is an efficient alternative to chemical fungicides in the inhibition of Phytophthora diseases in the soil, hence a balancing feedback loop reinforcing the soil system as natural capital.This article is published as Msayleb, N, R.S. Kanwar, J. van Leeuwen, and H. Wu. âOzonation efficacy in the treatment of soil-borne Phytophthora sojaein cultivating soybeansâ Scientific Pages of Environmental Studies 1, no. 1 (2017): 1-10. Posted with permission.</p