Comparative Acute Toxicity of Certain Pesticides Against Daphnia Longispina

Some of commercial pesticides used in Egypt were examined in laboratory against first generation of Daphnia longispina. The tested animals were collected from Kafr El-zayat district, Egypt and acclimatized in laboratory until used. The examined compounds were imidaclopride, indoxcarb and pyriproxyfen. It is evident from LC50 (96 hrs) values of the compounds that, indoxcarb is highly toxic, while pyriproxyfen is the lowest one. On the other hand, imidacloprid induced LC50 (3.4 ppm; 24 hrs) and decreased to 104 ppm after 96 hrs. The obtained data declared that, Daphnia is a very useful tool for screening pesticides pollution and evaluate their acute toxicity

Comparative Acute Toxicity of Certain Pesticides Against Daphnia Longispina

Some of commercial pesticides used in Egypt were examined in laboratory against first generation of Daphnia longispina. The tested animals were collected from Kafr El-zayat district, Egypt and acclimatized in laboratory until used. The examined compounds were imidaclopride, indoxcarb and pyriproxyfen. It is evident from LC50 (96 hrs) values of the compounds that, indoxcarb is highly toxic, while pyriproxyfen is the lowest one. On the other hand, imidacloprid induced LC50 (3.4 ppm; 24 hrs) and decreased to 104 ppm after 96 hrs. The obtained data declared that, Daphnia is a very useful tool for screening pesticides pollution and evaluate their acute toxicity

Evaluation of freshwater heavy metals accumulation effect on oxidative stress, Metallothionein biosynthesis and histopathology of Procambarus clarkii (Girard,1985) collected from three locations in the Delta region, Egypt

Abstract Background In this study, the effect of heavy metals accumulation influence was evaluated on adult crayfish Procambarus clarkii (Decapoda, Astacidea) collected from three different Governmental locations (Kafr El-Shaikh, El-Menofya, and El-Gharbiya) of the Egyptian Delta. The activity of super oxidase dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) of gills, hepatopancreas, and muscle tissue were measured. SDS Polyacrylamide gel electrophoresis (SDS-PAGE) and West blotting technique were performed to detect MT Protein expression. Results The results revealed that Kafr El-Shaikh reflected the highest Superoxide dismutase (SOD), Catalase, and Glutathione S-transferase (GST) activity levels (97.2 u/100 mg, 28.5 u/100 mg, and 8.3 nmol mg (-1) protein min (-1) respectively. Superior protein polymorphism % (30%) remarked collected Freshwater crayfish P. clarkii from Kafr El-Shaikh location. Varied protein polymorphism % was shown between collected crayfish from El-Menofya, and El-Gharbiya locations (5.5 and 6.2 respectively) Increasing Metallothioneins intensity (15.4%) for collected Freshwater crayfish Procambarus clarkii from Kafr El-Shaikh Location. Conclusion Heavy metal stress influences antioxidant status and also induces increasing Metallothioneins intensity, especially samples that were collected from the Kafr El-Shaikh area

An attempt to improve the proximate composition of local Artemia strain (Wadi El Natrun, Egypt)

Abstract Background Comparative analysis of essential amino acids and fatty acid of enriched and unenriched Artemia spp. collected from Wadi El Natrun, Elbeheira, Egypt, was investigated. Results The obtained results indicated that Leucine recorded the highest concentration (17.22%, 11.4%) in unenrichment A. tunisiana and A. franciscana, respectively followed by arginine (10.31%, 9.6%); however, glutamic acid recorded the lowest concentration (0.05%) in A. tunisiana. Statistical analysis showed no significant differences in some amino acid concentrations (P < 0.001) in both A. tunisiana and A. franciscana as arginine, valine, proline, and aspartic acid. Other amino acids were highly significantly fluctuated as glycine, leucine, histidine, phenylalanine, glutamic, and tyrosine; they were high in A. franciscana. Aspartic acid was significantly higher in unenrichment nauplii. Regarding the comparison between A. franciscana and enrichment A. tunisiana, the data showed non-significant improvement for the majority of the recorded amino acids. The analysis of unsaturated fatty acids n-3 and n-6 families after 18 and 24 h of enrichment Artemia was achieved. The composition of unsaturated fatty acids was significantly increased after enrichment to 79.7 mg/g DW instead of 46.5 in newly hatched nauplii. Saturated fatty acids were also significantly increased from 20 to 25.28 mg/g DW. The enrichment process was significant (P < 0.001) in nauplii enriched with fatty acids for 18 h. The more pronounced effect of duration was the amount of energy as it was 33.17 kJ/g after 18 h, while it was 45.693 kJ/g after 24 h incubation. The ratio of ascorbic acid, due to Artemia enrichment by vitamin C for 24 h, was increased from 853 to 3227 μg/g dry weight after enrichment. Conclusion To conclude, enrichment with cod liver oil and vitamin C obviously affected the chemical composition of local Egyptian Artemia (A. tunisiana)