Micronucleus analysis in buccal swabs of paddy farmers from the East Coast of Malaysia

Pesticide exposure may cause genotoxic effects by inducing the formation of micronucleus (Mn). Mn are fragments of chromosomes that remains after cells division. The increase in Mn may increase the risk of cancer formation. Our study aimed to determine the effects of lifestyle and pesticide exposure on the formation of Mn in epithelial cells from buccal swabs among paddy farmers in Malaysia. About 40 farmers who were exposed to pesticides were chosen as subjects and 30 personnels whose not directly exposed to pesticides, were chosen as the control group. Demographic and anthropometric data were obtained from questionnaires developed. Analysis of Mn formation was done using Giemsa staining (10% v/v) and the frequency of Mn formation was scored from 1000 cells per sample. Kruskal-Wallis test done between Mn frequency with age group showed a significant increase (p < 0.05) in Mn frequency in farmers as compared to the control in the age group of 30-39 , 40-49 years, and 50-59 years. Significant increased (p < 0.05) were observed between Mn frequency groups of normal BMI, pre-obese, and grade 1 obese as compared to control. Significant increase of Mn frequency (p < 0.01) was also seen among smokers and farmer’s group (15.39 ± 3.34) as compared to controls (4.76 ± 1.26). The maximum numbers of Mn found in farmers are 7 Mn per cell whereas for control group is only 3 Mn. However, most farmers had only 1 Mn (81.75 ± 6.42%) and 2 Mn (15.28 ± 5.14%). Mn frequency with the duration of exposure to pesticides in a month and the use of PPE revealed no significant difference (p = 0.27). In conclusion, the increased frequency of Mn was influenced by age, gender, BMI and smoking status of farmers besides commonly repeated duration of exposures and the use of PPE. Further studies are needed to analyze the causes of an increased in Mn among farmers

Penentuan tahap logam berat, hafazan al-Quran dan kecerdasan (IQ) dalam kalangan pelajar tahfiz di Selangor

Sekolah tahfiz merupakan institusi yang mengkhususkan pendidikan kepada pelajar yang boleh menghafaz serta mengingati keseluruhan al-Quran. Aktiviti menghafaz al-Quran dengan teknik pengucapan berulang kali akan membantu meningkatkan keupayaan otak untuk memproses, mengingati maklumat dan membina memori. Kehadiran logam berat akan memberi kesan kepada sistem saraf serta mengganggu fungsi sistem saraf pusat dan periferi di mana akan seterusnya menyebabkan berlakunya kecelaan fungsi mental dan kognitif. Keupayaan untuk belajar, mengingati atau menghafaz, penggunaan bahasa dan untuk memahami sesuatu akan terganggu serta mengurangkan IQ dan perhatian. Kajian keratan rentas telah dilakukan bagi mengukur dan mengenal pasti hubungan di antara tahap logam berat, hafazan al-Quran dan tahap kecerdasan (IQ) dalam kalangan pelajar di sekolah tahfiz berbanding sekolah bukan tahfiz terpilih di Selangor. Kepekatan logam berat ditentukan melalui analisa sampel kuku dan rambut pelajar dengan menggunakan Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Ujian kecerdasan Wechsler Adult Intelligence Scale Edisi Kedua (WASI-II) digunakan untuk mengukur IQ pelajar. Borang soal selidik yang digunakan untuk mengumpulkan data demografik dianalisa menggunakan SPSS versi 23.0. Berdasarkan ujian korelasi Pearson yang dilakukan, terdapat korelasi negatif yang sangat lemah tetapi signifikan antara logam mangan dalam sampel rambut dengan tahap hafazan al-Quran (r = -0.178, p = 0.017). Selain itu, terdapat korelasi positif yang lemah tetapi signifikan antara IQ dengan tahap hafazan al-Quran (r = 0.375, p = < 0.001). Kesimpulannya, semakin tinggi kepekatan logam mangan akan menyebabkan penurunan tahap hafazan al-Quran serta apabila semakin tinggi tahap hafazan al-Quran akan menyebabkan tahap kecerdasan (IQ) juga semakin meningkat

Adoption of the Mobile Health Screening Programme for Farming Communities: A Study Among Pesticide-Exposed Farmers from North East of Peninsular Malaysia

Health awareness promotion among farming communities are important for a sustainable agriculture activities. A crosssectional study was conducted to assess health status among farming communities in Cameron Highlands, Pahang (n = 61) and Bachok and Pasir Puteh, Kelantan (n = 143). Mobile Health Screening Programme composed of assessment of blood glucose, blood cholesterol, haemoglobin, blood pressure, pterygium, lung function and nerve conductive velocity was utilized. Our results indicate that the percentage of Cameron Highland’s farmers with hyperglycaemia, systolic hypertension, diastolic hypertension and anaemia were 8.2%, 14.8%, 11.5% and 8.2%, respectively. However, higher percentage of farmers in Bachok and Pasir Puteh, Kelantan with hyperglycaemia (32.8%), hypercholesterolaemia (83%), anaemia (24.2%) and systolic hypertension (41.9%) were observed. Pterygium was positive for 88.6% of farmers in Cameron Highlands and 94.4% in Bachok and Pasir Puteh. Lung function test shows that 61.7% and 11.4% of farmers in Cameron Highlands had restrictive and obstructive lung, respectively. In Bachok and Pasir Puteh, a total of 19.8%, 55.5% and 23.9% of farmers were found to have obstructive, restrictive and combined obstructive and restrictive lung, respectively. Current Perception Threshold (CPT) value which indicate nerve conductive velocity were signifi cantly increased (p < 0.05) among Cameron Highland’s farmers for both median and peroneal nerve at all frequencies (5 Hz, 250 Hz and 2000 Hz). In Bachok and Pasir Puteh, the values of the CPT for median nerve was signifi cantly increased (p < 0.05) for all frequencies (5, 250 and 2000 Hz). Meanwhile, a signifi cant increased (p < 0.05) was observed for the CPT values for peroneal nerve at the frequencies of 250 and 2000 Hz as compared to control groups. In conclusion, analysis revealed different health problem among the studied farming communities which could be infl uenced by the differences in farming practices. Thus, employed Mobile Health Screening Programme offers a monitoring approach that could highlight the need for suitable health services and awareness programmes for different farming communities

Faktor yang Mempengaruhi Paras Kromium dalam Kalangan Petani di Daerah Bachok dan Pasir Puteh, Kelantan

Kromium merupakan mineral yang penting dan kofaktor insulin yang memainkan peranan penting sebagai hormon yang membantu dalam regulasi gula dalam darah. Penduduk Kelantan sering dikaitkan dengan pengambilan makanan berasaskan gula. Petani merupakan golongan yang berisiko tinggi kerana pendedahan terhadap pestisid dan pengambilan makanan bergula boleh mempengaruhi paras kromium. Objektif kajian ini adalah untuk mengetahui status paras kromium dalam kalangan petani yang terdedah kepada pestisid dan baja kimia di Kelantan. Kajian ini adalah kajian keratan rentas yang dilakukan di Bachok dan Pasir Puteh, Kelantan. Responden adalah seramai 113 petani yang terdedah kepada pestisid atau baja kimia tidak kurang daripada satu tahun. Subjek ditemu bual menggunakan soal selidik pengetahuan, sikap dan amalan (KAP) yang telah divalidasi untuk informasi berkaitan data demografi k. Sampel kuku dan rambut telah dianalisia dengan menggunakan kaedah pencernaan asid dan Inductively Coupled Plasma Mass Spectroscopy (ICPMS) untuk mendapatkan paras kromium. Hasil kajian menunjukkan 81.4% adalah petani lelaki dan 18.6% adalah perempuan. Paras kromium kuku (125.82 ± 47.81 μg/L) dan rambut (39.63 ± 5.70 μg/L) petani adalah lebih rendah berbanding julat piawai kuku (6200 μg/L) dan rambut (100-2500 μg/L). Tiada perbezaan paras kromium yang signifi kan (p > 0.05) menurut jantina, umur, glukosa darah, tempoh pendedahan pestisid dan pemakanan. Petani yang merokok menunjukkan paras kromium yang lebih rendah (p < 0.05) berbanding petani yang tidak merokok. Kesimpulannya, paras unsur kromium petani di Bachok dan Pasir Puteh adalah rendah berbanding julat normal dan petani harus berhenti merokok kerana merokok akan merendahkan paras kromium

Paras selenium, zink dan kromium dalam kalangan pesawah yang terdedah pestisid di MADA, Perlis dan komuniti nelayan di Mersing, Johor

Pendedahan terhadap pestisid dapat menyebabkan penurunan paras unsur surih di dalam badan manusia. Unsur surih memainkan peranan penting dalam metabolisma tubuh. Kajian ini dijalankan untuk mengkaji paras unsur surih selenium, zink dan kromium dalam kalangan pesawah yang terdedah kepada pestisid di Wilayah I, MADA, Perlis. Kajian keratan rentas ini melibatkan 70 orang pesawah dan 57 orang yang tinggal di perkampungan nelayan sebagai kumpulan kawalan yang berumur di antara 21 hingga 80 tahun. Maklumat sosiodemografi pesawah dilakukan melalui temuduga borang soal selidik yang telah divalidasi. Pemeriksaan tekanan darah dan glukosa darah pesawah dilakukan. Paras selenium, zink dan kromium sampel kuku dan rambut dianalisis dengan menggunakan kaedah penghadaman asid dan mesin Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Hasil kajian mendapati bahawa paras selenium pada rambut (5.11 ± 17.05 µg/L) dan kuku (4.92 ± 2.17 µg/L) adalah lebih rendah secara signifikannya (p < 0.05) berbanding paras selenium pada rambut (15.67 ± 10.59 µg/L) dan kuku (6.67 ± 2.81 µg/L) kumpulan kawalan. Paras kromium pada rambut (31.83 ± 15.17 µg/L) dan kuku (87.64 ± 23.30 µg/L) kumpulan pesawah juga didapati lebih rendah secara signifikannya (p < 0.05) berbanding paras kromium pada rambut (85.19 ± 56.90 µg/L) dan kuku (99.36 ± 56.89 µg/L) pada kumpulan kawalan. Walau bagaimanapun, tiada perbezaan paras unsur surih yang signifikan (p > 0.05) menurut tempoh pendedahan pestisid. Kesimpulannya, paras selenium dan kromium pada kuku dan rambut pesawah yang terdedah kepada pestisid adalah lebih rendah berbanding kumpulan komuniti nelayan

Spatial Distribution of COVID-19 Infected Cases in Kelantan, Malaysia

Kota Bharu city in Kelantan, Malaysia was reported with the highest cases of coronavirus disease 2019 (COVID-19) among other districts. Kota Bharu is the capital city of Kelantan, which acts as the administrative, commercial, and financial areas. A large population pool may become a potential carrier for disease transmission to become an epidemic. However, the impact of population density on the COVID-19 outbreak in Malaysia is still unknown and undiscovered. Therefore, this study investigates the impact of population density on COVID-19 as a potential virus transmission carrier using linear regression models. The chances of formulating new strategies for combating COVID-19 are higher when the driver of transmission potential is identified. This study shows that the highest value of infected area density is in Kota Bharu (0.76), while the infected risk area was highest in Jeli (0.33). This study found that there is a strong relationship between COVID-19 infection cases in Kelantan and population density (R2 which is 0.845). Therefore, high population density was identified as a potential driver of transmission of COVID-19 outbreak. Understanding the potential drivers of the disease in a local setting is very important for better preparation and management. The outcome of the study can aid in the development of a new analytical model for strategic planning of Zero COVID-19 for securing the public health and wellness, both social and economic, by researchers, scientists, planners, resource managers, and decision-makers