Neutrophil reactive oxygen species (ROS) generation in patients across the continuum of sepsis

ROS generation was measured by 2',7'dichlorofluorescein (DCFH) metabolism. Neutrophils were gated based on FSC versus SSC parameters and the expression of CD14 on cell surface. ROS generation was analyzed in histograms and expressed as the geometric mean fluorescence intensity (GMFI). *p < 0.01 compared to healthy volunteers; #p < 0.05 compared to the severe sepsis group; §p < 0.05 compared to the septic shock group.<p><b>Copyright information:</b></p><p>Taken from "Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis"</p><p>http://ccforum.com/content/12/1/R25</p><p>Critical Care 2008;12(1):R25-R25.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374621.</p><p></p

Correlation between formyl-methionyl-leucyl-phenylalanine (fMLP)-induced reactive oxygen species (ROS) generation and sepsis-associated organ failure assessment (SOFA) score in neutrophils and monocytes in patients with severe sepsis and septic shock

<p><b>Copyright information:</b></p><p>Taken from "Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis"</p><p>http://ccforum.com/content/12/1/R25</p><p>Critical Care 2008;12(1):R25-R25.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374621.</p><p></p

Correlation between neutrophil and monocyte oxidative metabolism in septic patients at baseline (A) and after stimuli with phorbol myristate acetate (PMA) (B), formyl-methionyl-leucyl-phenylalanine (fMLP) (C), lipopolysaccharide (LPS) (D) and (E)

<p><b>Copyright information:</b></p><p>Taken from "Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis"</p><p>http://ccforum.com/content/12/1/R25</p><p>Critical Care 2008;12(1):R25-R25.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374621.</p><p></p

Dot plots depict the gating strategies for CD25and CD25cells (A); CD127(B); and CTLA-4 expression (C) are shown

The gates for CD25and CD4CD25T cells were set based on the expression level of CD25 on CD4T cells, where the pattern of CD25 expression is more distinct. Accordingly, CD4CD25T cells were defined as those expressing CD25 at a level higher than the bulk CD4CD25population. For D-K, individual results are represented in symbols, and the line represents the mean. (D) % CD4CD25T cells; (E) % CD4CD25T cells; (F) % CD4CD25CTLA-4T cells; (G) % of CD4CD25CTLA-4T cells; (H) % CD4CD25CD127T cells; (I) % CD4CD25CD127T cells; (J) % CD4CD25GITRT cells; and (K) % CD4CD25GITRT cells.<p><b>Copyright information:</b></p><p>Taken from "The frequency of CD127expressing CD4CD25T regulatory cells is inversely correlated with human T lymphotrophic virus type-1 (HTLV-1) proviral load in HTLV-1-infection and HTLV-1-associated myelopathy/tropical spastic paraparesis"</p><p>http://www.biomedcentral.com/1471-2172/9/41</p><p>BMC Immunology 2008;9():41-41.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2515103.</p><p></p