Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds

Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds

Comparative essential oil composition of flowers and leaves of Warionia saharae Benth & Coss.

International audienceThe chemical composition of leaves and flowers of Warionia saharae Bent.&Coss., have been examined by GC and GC–MS, the identified components constituting 95.3% and 84.1 % of the leaves and flowers oils respectively. The main constituents of leaves oil were linalool (27.7%), beta-eudesmol (25.7%), (E)-nerolidol (8.4%), geraniol (7.1%), alpha-terpineol (5.5%) , nerol (2.4%), eremoligenol (2.1%), alpha-agarofurane (1.7%) , 1,8-cineol (1.4%) and limonene (1.3%) while nerolidol (29.6%), beta-eudesmol (28.5%), farnesol (8.2%), linalool (3.4%), beta-caryophyllene (3.1%), eremoligenol (2.5%), acorenone (2.1%) were established as the main components of the flowers. These results differ from the few previous studies reported on this species

Antioxidant activity and chemical constituents of Warionia saharae Benth. & Coss. (Compositae) from Algeria.

International audiencePhytochemical investigations of chloroform and ethyl acetate extracts from the aerial parts of Warionia saharae Benth. &amp; Coss. from Algeria resulted in the isolation of ten known compounds: β-sitosterol, stigmasterol, scopoletin, cirsimaritin, chrysoeriol, hispidulin, luteolin, esculetin, (2R,3R) taxifolin, quercetin. Their structures were elucidated on the basis of extensive spectroscopic analysis and comparison with the related known compounds. The EtOAc extract which was evaluated for DPPH radical scavenging activity and of lipid peroxide inhibition showed potent antioxidant ability compared to the quercetin and ascorbic acid used as positive control.</p

Antioxidant activity and chemical constituents of Warionia saharae Benth. & Coss. (Compositae) from Algeria.

International audiencePhytochemical investigations of chloroform and ethyl acetate extracts from the aerial parts of Warionia saharae Benth. &amp; Coss. from Algeria resulted in the isolation of ten known compounds: β-sitosterol, stigmasterol, scopoletin, cirsimaritin, chrysoeriol, hispidulin, luteolin, esculetin, (2R,3R) taxifolin, quercetin. Their structures were elucidated on the basis of extensive spectroscopic analysis and comparison with the related known compounds. The EtOAc extract which was evaluated for DPPH radical scavenging activity and of lipid peroxide inhibition showed potent antioxidant ability compared to the quercetin and ascorbic acid used as positive control.</p