Effect of Consuming Iodized Salt on Fertility Indices in Male Adult Rats

Introduction: Today about 27.4 percent of female 15-44 years and 1 percent of female in fertility age are affected by infertility. Iodine is a rare element that is essential for the synthesis of thyroid hormones. Concentration of the thyroid hormones in blood under the influence of iodine intake and changes in thyroid hormones levels interact with reproductive system. Today, all the people of Iran consuming iodized salt regardless of iodine status in their body. In this study according to high prevalence of the infertility among young couples, iodized salt intake on fertility in male rats were investigated. Materials and Methods: In this study 20 male and 20 female adult Wistar rats were used. Twenty male adult Wistar rats were randomly divided into 2 groups. Including the control group and treatment group that received iodine and female adult Wistar were fed with a regular diet. Five male rats from each group were killed at the end of the fourth weeks in order to evaluate the possible effect of iodized salt on sperm analysis and weight of testis. After a month, male and female rats were placed in pairs in separate cages and their offspring were investigated in terms of number, gender and health. Results: The result of this study showed that the number of healthy offspring of treated male rats was significantly lower than the control group. Conclusion: Due to the negative effect of excessive iodine intake on fertility rate, it is recommended to couples to perform functional tests of their thyroid glands before intake of iodized salts

Wound Healing Effect of Aqueous Extract of Rhus Coriaria in Rat

Introduction: Wound healing is one of the most complicated biological events after birth that causes dead tissue replace with new one. Any defect in wound healing, can lead to chronic skin disorders causing severe loss of quality of life and even death. So far, a drug that could effectively promote and advance the natural process of wound healing in the body, has not been introduced. Treatment of wounds and burns, is still one major issue, even in developed countries. Due to importance and value of antioxidant and anti-inflammatory effects on wound healing, and the presence of these activities in some medicinal plants, in this study, the healing effects of aqueous extract of &nbsp;Rhus coriaria fruit, was examined. &nbsp; Materials and Methods: After creating a full-thickness skin wound on the back of 45 male Sprague-Dawley rats, they were randomly divided into three groups: treatment, control or positive control. After 24 hours, the treatment group received 0.5 gram of the fruit extract, the positive control group treated with 2%mupirocin ointment&nbsp; and the control group received no treatment. Wound healing rates were calculated on days 0, 3, 5, 8, 10, 12 and 15 post-wounding and the wound tissues were harvested at 5, 10, and 15 days for histological and biochemical analysis. Myeloperoxidase (MPO) activity was used to determine the anti-oxidant and anti- inflamatory activity of the pklant extract. The results were statistically analysed using SPSS software. Results: The percentage of the wound contraction was significantly increased in days 10, 12 and 15 in the treated group compared to the control group(P<0.01). A significant increase in the assigned histological scores was observed at days 10 and 15&nbsp; in the treated group compared to the control group (P<0.005 and P<0.05 respectively). This extract significantly decreased the MPO activity 10 days after treatment (P<0.05). Conclusion: These data provide evidence that Rhus coriaria extract accelerates cutaneous wound healing and its effect may be due to the increased re-epithelialization, collagen deposition and decreased MPO activity and oxidative damage in wound and so it can be used as a therapeutic agent for wound healing

The effect of calcium on the adhesion of Streptococcus mutans to Human Gingival Epithelial Cells in the presence of probiotic bacteria Lactobacillus plantarum and Lactobacillus salivarius

Introduction: Oral pathogens are among the most common bacterial infections in humans. The adhesion of probiotic bacteria to epithelial cells plays an important role in inhibiting the growth and binding of pathogenic bacteria. We aim to investigate the effect of calcium carbonate on the binding of Streptococcus mutans to Human Gingival Epithelial Cells (HGECs) in the presence of the probiotic bacteria Lactobacillus plantarum and Salivarius. Methods and materials: Experiments are performed in the presence of calcium and probiotic bacteria and HGECs in duplicate and three times separately. The difference in the amount of Streptococcus mutans in HGECs is determined by the presence and absence of Lactobacillus plantarum or salivarius and calcium or both by dilution and culture procedures. Results: Simple effects analysis showed that the survival rate of HGECs in all doses was significantly higher than negatives (p < .001). The results indicated that the survival rate in the single dose of L. salivarius (0.617 ± 0.003) and L. plantarum (0.652 ± 0.002) was significantly higher than the combined doses. Conclusion: This study clearly demonstrates that calcium and probiotics from Lactobacillus salivarius and plantarum alone can improve the survival of HGECs in the presence of Streptococcus mutans. But the combined function of these therapeutic factors is not optimal and even reduces their therapeutic efficacy. © 202

Novel targets in rectal cancer by considering lncRNA�miRNA�mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial

We aimed to explore the lncRNA�miR�mRNA network in response to Lactobacillusacidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA�miR�mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L.acidophilus consumption was associated with improved expression of the lncRNA�miR�mRNA network, which may provide novel monitoring and therapeutic approaches. © 2022, The Author(s)