The role of pH on the thermodynamics and kinetics of muscle biochemistry: An in vivo study by 31P-MRS in patients with myo-phosphorylase deficiency

AbstractIn this study we assessed ΔG′ATP hydrolysis, cytosolic [ADP], and the rate of phosphocreatine recovery using Phosphorus Magnetic Resonance Spectroscopy in the calf muscle of a group of patients affected by glycogen myo-phosphorylase deficiency (McArdle disease). The goal was to ascertain whether and to what extent the deficit of the glycogenolytic pathway would affect the muscle energy balance. A typical feature of this pathology is the lack of intracellular acidosis. Therefore we posed the question of whether, in the absence of pH decrease, the rate of phosphocreatine recovery depends on the amount of phosphocreatine consumed during exercise. Results showed that at the end of exercise both [ADP] and ΔG′ATP of patients were significantly higher than those of matched control groups reaching comparable levels of phosphocreatine concentration. Furthermore, in these patients we found that the rate of phosphocreatine recovery is not influenced by the amount of phosphocreatine consumed during exercise. These outcomes provide experimental evidence that: i) the intracellular acidification occurring in exercising skeletal muscle is a protective factor for the energy consumption; and ii) the influence of pH on the phosphocreatine recovery rate is at least in part related to the kinetic mechanisms of mitochondrial creatine kinase enzyme

Key Areas For Conserving United States\u27 Biodiversity Likely Threatened By Future Land Use Change

A major challenge for biodiversity conservation is to mitigate the effects of future environmental change, such as land use, in important areas for biodiversity conservation. In the United States, recent conservation efforts by The Nature Conservancy and partners have identified and mapped the nation\u27s Areas of Biodiversity Significance (ABS), representing the best remaining habitats for the full diversity of native species and ecosystems, and thus the most important and suitable areas for the conservation of native biodiversity. Our goal was to understand the potential consequences of future land use changes on the nation\u27s ABS, and identify regions where ABS are likely to be threatened due to future land use expansion. For this, we used an econometric-based model to forecast land use changes between 2001 and 2051 across the conterminous U. S. under alternative scenarios of future land use change. Our model predicted a total of similar to 100,000 to 160,000 km(2) of natural habitats within ABS replaced by urban, crop and pasture expansion depending on the scenario (5% to 8% habitat loss across the conterminous U.S.), with some regions experiencing up to 30% habitat loss. The majority of the most threatened ABS were located in the Eastern half of the country. Results for our different scenarios were generally fairly consistent, but some regions exhibited notable difference from the baseline under specific policies and changes in commodity prices. Overall, our study suggests that key areas for conserving United States\u27 biodiversity are likely threatened by future land use change, and efforts trying to preserve the ecological and conservation values of ABS will need to address the potential intensification of human land uses

Action Observation Therapy for Arm Recovery after Stroke: A Preliminary Investigation on a Novel Protocol with EEG Monitoring

This preliminary study introduces a novel action observation therapy (AOT) protocol associated with electroencephalographic (EEG) monitoring to be used in the future as a rehabilitation strategy for the upper limb in patients with subacute stroke. To provide initial evidence on the usefulness of this method, we compared the outcome of 11 patients who received daily AOT for three weeks with that of patients who undertook two other approaches recently investigated by our group, namely intensive conventional therapy (ICT), and robot-assisted therapy combined with functional electrical stimulation (RAT-FES). The three rehabilitative interventions showed similar arm motor recovery as indexed by Fugl-Meyer’s assessment of the upper extremity (FMA_UE) and box and block test (BBT). The improvement in the FMA_UE was yet more favourable in patients with mild/moderate motor impairments who received AOT, in contrast with patients carrying similar disabilities who received the other two treatments. This suggests that AOT might be more effective in this subgroup of patients, perhaps because the integrity of their mirror neurons system (MNS) was more preserved, as indexed by EEG recording from central electrodes during action observation. In conclusion, AOT may reveal an effective rehabilitative tool in patients with subacute stroke; the EEG evaluation of MNS integrity may help to select patients who could maximally benefit from this intervention

Evidence That Nasal Insulin Induces Immune Tolerance to Insulin in Adults With Autoimmune Diabetes

OBJECTIVE: Insulin in pancreatic β-cells is a target of autoimmunity in type 1 diabetes. In the NOD mouse model of type 1 diabetes, oral or nasal administration of insulin induces immune tolerance to insulin and protects against autoimmune diabetes. Evidence for tolerance to mucosally administered insulin or other autoantigens is poorly documented in humans. Adults with recent-onset type 1 diabetes in whom the disease process is subacute afford an opportunity to determine whether mucosal insulin induces tolerance to insulin subsequently injected for treatment. RESEARCH DESIGN AND METHODS: We randomized 52 adults with recent-onset, noninsulin-requiring type 1 diabetes to nasal insulin or placebo for 12 months. Fasting blood glucose and serum C-peptide, glucagon-stimulated serum C-peptide, and serum antibodies to islet antigens were monitored three times monthly for 24 months. An enhanced ELISpot assay was used to measure the T-cell response to human proinsulin. RESULTS: β-Cell function declined by 35% overall, and 23 of 52 participants (44%) progressed to insulin treatment. Metabolic parameters remained similar between nasal insulin and placebo groups, but the insulin antibody response to injected insulin was significantly blunted in a sustained manner in those who had received nasal insulin. In a small cohort, the interferon-γ response of blood T-cells to proinsulin was suppressed after nasal insulin. CONCLUSIONS: Although nasal insulin did not retard loss of residual β-cell function in adults with established type 1 diabetes, evidence that it induced immune tolerance to insulin provides a rationale for its application to prevent diabetes in at-risk individuals

Economic-based projections of future land use in the conterminous United States under alternative policy scenarios

Land-use change significantly contributes to biodiversity loss, invasive species spread, changes in biogeochemical cycles, and the loss of ecosystem services. Planning for a sustainable future requires a thorough understanding of expected land use at the fine spatial scales relevant for modeling many ecological processes and at dimensions appropriate for regional or national-level policy making. Our goal was to construct and parameterize an econometric model of land-use change to project future land use to the year 2051 at a fine spatial scale across the conterminous United States under several alternative land-use policy scenarios. We parameterized the econometric model of land-use change with the National Resource Inventory (NRI) 1992 and 1997 land-use data for 844 000 sample points. Land-use transitions were estimated for five land-use classes (cropland, pasture, range, forest, and urban). We predicted land-use change under four scenarios: business-as-usual, afforestation, removal of agricultural subsidies, and increased urban rents. Our results for the business-as-usual scenario showed widespread changes in land use, affecting 36% of the land area of the conterminous United States, with large increases in urban land (79%) and forest (7%), and declines in cropland (\-16%) and pasture (\-13%). Areas with particularly high rates of land-use change included the larger Chicago area, parts of the Pacific Northwest, and the Central Valley of California. However, while land-use change was substantial, differences in results among the four scenarios were relatively minor. The only scenario that was markedly different was the afforestation scenario, which resulted in an increase of forest area that was twice as high as the business-as-usual scenario. Land-use policies can affect trends, but only so much. The basic economic and demographic factors shaping land-use changes in the United States are powerful, and even fairly dramatic policy changes, showed only moderate deviations from the business-as-usual scenario. Given the magnitude of predicted land-use change, any attempts to identify a sustainable future or to predict the effects of climate change will have to take likely land-use changes into account. Econometric models that can simulate land-use change for broad areas with fine resolution are necessary to predict trends in ecosystem service provision and biodiversity persistence. © 2012 by the Ecological Society of America

Projected land-use change impacts on ecosystem services in the United States

Providing food, timber, energy, housing, and other goods and services, while maintaining ecosystem functions and biodiversity that underpin their sustainable supply, is one of the great challenges of our time. Understanding the drivers of land-use change and how policies can alter land-use change will be critical to meeting this challenge. Here we project land-use change in the contiguous United States to 2051 under two plausible baseline trajectories of economic conditions to illustrate how differences in underlying market forces can have large impacts on land-use with cascading effects on ecosystem services and wildlife habitat. We project a large increase in croplands (28.2 million ha) under a scenario with high crop demand mirroring conditions starting in 2007, compared with a loss of cropland (11.2 million ha) mirroring conditions in the 1990s. Projected land-use changes result in increases in carbon storage, timber production, food production from increased yields, and \u3e10% decreases in habitat for 25% of modeled species. We also analyze policy alternatives designed to encourage forest cover and natural landscapes and reduce urban expansion. Although these policy scenarios modify baseline land-use patterns, they do not reverse powerful underlying trends. Policy interventions need to be aggressive to significantly alter underlying land-use change trends and shift the trajectory of ecosystem service provision

Current And Future Land Use Around A Nationwide Protected Area Network

Land-use change around protected areas can reduce their effective size and limit their ability to conserve biodiversity because land-use change alters ecological processes and the ability of organisms to move freely among protected areas. The goal of our analysis was to inform conservation planning efforts for a nationwide network of protected lands by predicting future land use change. We evaluated the relative effect of three economic policy scenarios on land use surrounding the U.S. Fish and Wildlife Service\u27s National Wildlife Refuges. We predicted changes for three land-use classes (forest/range, crop/pasture, and urban) by 2051. Our results showed an increase in forest/range lands (by 1.9% to 4.7% depending on the scenario), a decrease in crop/pasture between 15.2% and 23.1%, and a substantial increase in urban land use between 28.5% and 57.0%. The magnitude of land-use change differed strongly among different USFWS administrative regions, with the most change in the Upper Midwestern US (approximately 30%), and the Southeastern and Northeastern US (25%), and the rest of the U.S. between 15 and 20%. Among our scenarios, changes in land use were similar, with the exception of our restricted-urban-growth\u27\u27 scenario, which resulted in noticeably different rates of change. This demonstrates that it will likely be difficult to influence land-use change patterns with national policies and that understanding regional land-use dynamics is critical for effective management and planning of protected lands throughout the U.S

Economic-based Projections Of Future Land Use In The Conterminous United States Under Alternative Policy Scenarios

The article presents a study which constructs and parameterizes an econometric model of land-use change to project future land use to the year 2051 at a fine spatial scale across the conterminous U.S. under several alternative land-use policy scenarios. It parameterizes the econometric model of land-use change with the National Resource Inventory (NRI) 1992 and 1997 land-use data for 844 000 sample points

The global wildland–urban interface

The wildland–urban interface (WUI) is where buildings and wildland vegetation meet or intermingle1,2. It is where human–environmental conflicts and risks can be concentrated, including the loss of houses and lives to wildfire, habitat loss and fragmentation and the spread of zoonotic diseases3. However, a global analysis of the WUI has been lacking. Here, we present a global map of the 2020 WUI at 10 m resolution using a globally consistent and validated approach based on remote sensing-derived datasets of building area4 and wildland vegetation5. We show that the WUI is a global phenomenon, identify many previously undocumented WUI hotspots and highlight the wide range of population density, land cover types and biomass levels in different parts of the global WUI. The WUI covers only 4.7% of the land surface but is home to nearly half its population (3.5 billion). The WUI is especially widespread in Europe (15% of the land area) and the temperate broadleaf and mixed forests biome (18%). Of all people living near 2003–2020 wildfires (0.4 billion), two thirds have their home in the WUI, most of them in Africa (150 million). Given that wildfire activity is predicted to increase because of climate change in many regions6, there is a need to understand housing growth and vegetation patterns as drivers of WUI change

Key areas for conserving United States’ biodiversity likely threatened by future land use change

A major challenge for biodiversity conservation is to mitigate the effects of future environmental change, such as land use, in important areas for biodiversity conservation. In the United States, recent conservation efforts by The Nature Conservancy and partners have identified and mapped the nation's Areas of Biodiversity Significance (ABS), representing the best remaining habitats for the full diversity of native species and ecosystems, and thus the most important and suitable areas for the conservation of native biodiversity. Our goal was to understand the potential consequences of future land use changes on the nation's ABS, and identify regions where ABS are likely to be threatened due to future land use expansion. For this, we used an econometric‐based model to forecast land use changes between 2001 and 2051 across the conterminous U.S. under alternative scenarios of future land use change. Our model predicted a total of ∼100,000 to 160,000 km2 of natural habitats within ABS replaced by urban, crop and pasture expansion depending on the scenario (5% to 8% habitat loss across the conterminous U.S.), with some regions experiencing up to 30% habitat loss. The majority of the most threatened ABS were located in the Eastern half of the country. Results for our different scenarios were generally fairly consistent, but some regions exhibited notable difference from the baseline under specific policies and changes in commodity prices. Overall, our study suggests that key areas for conserving United States' biodiversity are likely threatened by future land use change, and efforts trying to preserve the ecological and conservation values of ABS will need to address the potential intensification of human land uses