Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients

HIV-associated lipodystrophy (HIVLD) is a metabolic condition with an irregularity in the production of lipoprotein particles, and its occurrence varies among HIV-infected patients. MTP and ABCG2 genes have a role in the transport of lipoproteins. The polymorphisms of MTP -493G/T and ABCG2 34G/A affect its expression and influence the secretion and transportation of lipoproteins. Hence, we investigated the MTP -493G/T and ABCG2 34G/A polymorphisms in 187 HIV-infected patients (64 with HIVLD and 123 without HIVLD) along with 139 healthy controls using polymerase chain reaction (PCR)-restriction fragment length polymorphism and expression analysis using real-time PCR. ABCG2 34A allele showed an insignificantly reduced risk of LDHIV severity [P = 0.07, odds ratio (OR) = 0.55]. MTP -493T allele exhibited a non-significantly reduced risk for the development of dyslipidemia (P = 0.08, OR = 0.71). In patients with HIVLD, the ABCG2 34GA genotype was linked with impaired low-density lipoprotein levels and showed a reduced risk for LDHIV severity (P = 0.04, OR = 0.17). In patients without HIVLD, the ABCG2 34GA genotype was associated with impaired triglyceride levels with marginal significance and showed an increased risk for the development of dyslipidemia (P = 0.07, OR = 2.76). The expression level of MTP gene was 1.22-fold decreased in patients without HIVLD compared with that in patients with HIVLD. ABCG2 gene was upregulated 2.16-fold in patients with HIVLD than in patients without HIVLD. In conclusion, MTP -493C/T polymorphism influences the expression level of MTP in patients without HIVLD. Individuals without HIVLD having ABCG2 34GA genotype with impaired triglyceride levels may facilitate dyslipidemia risk

Spectrophotometric determination of vitamin C using Fe(II)-1-(2- pyridylazo )-2- naphthol complex

1303-1306<span style="font-size:14.0pt;mso-bidi-font-size:8.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">An extractive spectrophotometric procedure based on the complexation of reduced iron(II) with 1-(2- pyridylazo)-2- naphthol (PAN) for the estimation of micro amounts of vitamin C is described. The resulting complex is extracted into dichloromethane forming a reddish brown extract which shows an absorption band at 765 nm. Linear relationship between absorbance and concentration of ascorbic acid is observed up to 8.0 <span style="font-size:14.0pt;mso-bidi-font-size: 8.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:hiddenhorzocr;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">μ<span style="font-size:14.0pt;mso-bidi-font-size:8.0pt;font-family:HiddenHorzOCR; mso-hansi-font-family:" times="" new="" roman";mso-bidi-font-family:hiddenhorzocr;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">g ml-1<span style="font-size: 11.5pt;mso-bidi-font-size:5.5pt;font-family:Arial;mso-fareast-font-family:" times="" new="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">.  <span style="font-size:14.0pt; mso-bidi-font-size:8.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="">Interference studies of different substances including sugars, vitamins and amino acids, metal ions and organic acids have been carried out. The utility of the method has been tested by analyzing some of the marketed products of vitamin C and biological samples.</span

Extractive spectrophotometric determination of iron using 3-hydroxy-2-(2-thienyl) -4H chromen-4-one

552-553A highly sensitive method is described for the determination of iron (III). Iron(III) forms a brown coloured complex with 3-hydroxy-2-(2-thienyl)-4H-chromen-4-one (HTC) in acidic medium which is conveniently extracted into chloroform. The absorbance of the complex is measured at 415 nm. Beer's Law is obeyed up to 1.4 μg/ml with the molar absorptivity of 5.27×104 l/mol/cm. The method is quite simple, sensitive and selective and has been employed for analyzing some synthetic samples, chemicals and brazing solder

Radiological and Pathological Findings in a Case of Large Left Adrenal Myelolipoma

Adrenal myelolipomas are rare and uncommon benign tumours of the adrenal gland. They contain mature adipose tissue and a variable amount of haemopoietic tissue. Most lesions are small, asymptomatic and discovered incidentally. However larger lesions may be symptomatic. Here we describe the imaging and pathological findings in a case of large left adrenal myelolipoma. [Med-Science 2016; 5(1.000): 258-65

Obstructive coronary artery disease in women presenting with ischemic chest pain: Prevalence and risk determinants

Background: Women present more commonly with atypical angina, false-positive exercise test, and normal/nonobstructive coronary angiogram. It thus becomes essential to identify the predictors of obstructive coronary artery disease (CAD) in women to avoid unnecessary invasive angiograms. Methods: We prospectively recorded the data of consecutive 578 women who underwent coronary angiography in our institute for typical/atypical chest pain suspected to be secondary to CAD. Results: Normal coronaries/nonobstructive CAD were seen in 273 (47.2%) patients, whereas 305 (52.8%) patients were found to have obstructive CAD (P 55 years, smoking, diabetes mellitus, hypertension, low-density lipoprotein cholesterol >150 mg/dl, and high-density lipoprotein cholesterol <40 mg/dl were found to be independent predictors of obstructive CAD. Conclusion: These risk predictors can help in the reliable assessment of pretest likelihood of obstructive CAD in women, thereby avoiding many unnecessary invasive coronary angiograms. Further larger studies are warranted to validate these results and propose accurate prediction models for the diagnosis of obstructive CAD in women

Laparoscopic Myomectomy with Uterine Artery Ligation: Review Article and Comparative Analysis

Uterine leiomyomas are one of the most common benign smooth muscle tumors in women, with a prevalence of 20 to 40% in women over the age of 35 years. Although many women are asymptomatic, problems such as bleeding, pelvic pain, and infertility may necessitate treatment. Laparoscopic myomectomy is one of the treatment options for myomas. The major concern of myomectomy either by open method or by laparoscopy is the bleeding encountered during the procedure. Most studies have aimed at ways of reducing blood loss during myomectomy. There are various ways in which bleeding during laparoscopic myomectomy can be reduced, the most reliable of which is ligation of the uterine vessels bilaterally. In this review we propose to discuss the benefits and possible disadvantages of ligating the uterine arteries bilaterally before performing laparoscopic myomectomy