O outplacement : caracterização e novas tendências

Mestrado em Gestão de Recursos HumanosO outplacement é uma técnica de gestão de recursos humanos que tem como intuito auxiliar o colaborador em transição na carreira a ultrapassar o trauma do despedimento e a encontrar uma nova colocação. O presente estudo tem como objectivo caracterizar o outplacement, descrever as suas novas tendências, e também comparar as suas práticas entre Portugal e o Reino Unido através de estudos de caso. Optou-se pela pesquisa qualitativa, devido ao seu carácter descritivo e exploratório. Os resultados evidenciam semelhanças e diferenças no que concerne as práticas do outplacement nos dois países. A composição dos programas de outplacement e uso de indicadores para a medição da sua eficácia, a abordagem directa ao cliente enquanto estratégia de marketing, a complexidade do perfil do consultor de outplacement e a relação de proximidade que se estabelece entre o mesmo e o candidato, bem como o incentivo a comunicação contínua, são práticas de outplacement comuns a Portugal e ao Reino Unido. Foi ainda possível constatar que as principais diferenças entre os dois países se relacionam com a duração dos programas, uso exclusivo da tecnologia, existência de um mercado mais competitivo no Reino Unido e consequente maior necessidade de diferenciação por parte das empresas britânicas. Por fim, concluiu-se que o principal desafio que as empresas de outplacement enfrentam nos dias de hoje consiste na necessidade de educar o mercado acerca do real valor desta prática, articulada às vantagens da consultoria one-to-one.Outplacement is a human resource management practice which intends to assist employees in career transition to overcome the trauma of redundancy and find a new job. The present study aims to characterize outplacement, describe it?s new trends, and compare it?s practices between Portugal and the United Kingdom. Given the lack of in-depth knowledge on this subject in Portugal, it was resorted to qualitative research, due to its descriptive and exploratory nature. The method of investigation chosen was conducting case studies, related to companies analyzed in both countries. The results show similarities and differences concerning outplacement practices in both countries. The composition of outplacement programs and use of indicators to measure its effectiveness, the direct approach to customers as a marketing strategy, the outplacement consultant profile complexity and the close relationship established between consultant and candidate as well as encouraging continuous communication are common outplacement practices in Portugal and the United Kingdom. It was further established that the main differences between the two countries relate to program duration, exclusive use of the technology, the existence of a competitive market in the UK and consequently a greater need for differentiation by British companies. Finally, it is concluded that the main challenges that outplacement companies face nowadays is the need of educating the market about the real value of this practice, articulated to the benefits of one-to-one consulting

Reabilitação sustentável de edifícios de habitação

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Engenharia Civil – perfil de ConstruçãoO parque habitacional edificado é no momento actual um dos elementos que contribui grandemente para a degradação do ambiente. O nível de eficiência dos edifícios de habitação, no que respeita ao consumo de recursos naturais e ao conforto ambiental, leva a que hoje se possa considerar que o mesmo parque construído é insustentável. Deste modo, importa que se conheçam e determinem os problemas concretos do parque habitacional edificado de modo a se poder contribuir para a resolução desta situação. Conhecidos os principais problemas dos edifícios de habitação, bem como as suas causas, compreende-se que para a sua resolução existe a necessidade de adaptar o processo de reabilitação tradicional ao conceito de construção sustentável, a qual pode ser conseguida com base nos sistemas de avaliação e certificação da sustentabilidade na construção. A presente dissertação pretende então contribuir para que o processo de reabilitação de edifícios seja realizado de forma sustentável, com base na problemática referida

Glutathione detection using a fluorescence prove based on porphyrin-Hg complex

Glutathione (GSH) is an abundant tripeptide found in the almost all cells. GSH is relevant as antioxidant and in regulation of some carcinogenic mechanims. It's well known that disturbances in GSH homeostasis could unleash a cancer disease. Therefore, its necessary GSH effective proves to prevent future physiological alterations due to a decrease or increase of the tripectide concentrations. Herein, we report a turn-on fluorecence sensor based on a highly fluoresncence porphyrin derivated (chlorin) complexed with Hg (II).Methods: To study the formation of Hg(II)-Chlorine complex, a different concentrations of a mercury cloride (II) solution was added to a fixed concentration of chlorine and fitting to the Stern-Volmer ecuation. To study the response to glutathione, a certain ratio of Hg(II):Chlorine was exposed to increasing amounts of GSH. To determain the limit of detection (LOD), a quarter of the maximun slope of the curve is calculated for each sample.Results: Chlorine coordinates adequately to mercury producing a quenching according to the Stern-Volmer equation. For the determination of glutathione in water, a pH of 7.4 (physiological pH) was maintained and increasing amounts of glutathione were introduced for a fixed ratio of mercury-chlorine complex. At increasing amounts of glutathione, it is coordinated with mercury and releasing chlorine, resulting in an increase in fluorescence due to free chlorine. The response curve is sigmoidal in shape as glutathione first complexes with free mercury, and once this is depleted, GSH captures the mercury complexed to chlorine, producing the increase in fluorescence mentioned above.This experiment was repeated for different mercury:chlorine ratios (2:1, 5:1 and 10:1) with the 2:1 ratio having a lower detection limit of 0.01 uM. It is now intended to transfer this system to a titanium dioxide based film

ZIF-8 MOF used as optic sensor for toxic gases

Metal-Organic frameworks (MOFs) are crystalline organic-inorganic porous materials comprised of metal atoms or clusters coordinated by organic ligands. ZIF-8 (zeolitic imidazole framework), due to the abscense of an intricsic fluorescenc, has never been used as optical sensor. In this works, ZIF-8 has been dopped with Cd (II) atoms (2.5%), showing an huge enhacement of the fluorescent compared with the non-dopped ZIF-8. This new material is suitable for optical sensing, being sensitive to hydrogen sulfice gas, which causes quenching of the luminescence proportionally to its concentration.Methods: ZIF-8 was synthesized at room temperature mixing a methanol solution of Zn(NO3)2·6H2O with a methanol solution of 2-methyllimidazole (Hmim), with Zn (II), Hmim, methanol ratio of 1:8:700 [1].ZIF-8 was dopped with Cadmium by four different methods; the dopped samples was named ZIF-8-A1, ZIF-8-B, ZIF-8-A2 and ZIF-8-B2. The methodology followed was described by H. Fei et al. in [2], with some modifications. The difference among the varieties was the solvent and the post-treatment after mixing the compounds.The dopping method was accomplished by dissolving 0.4 mmol of Cd(NO3)2 and 20mg of ZIF-8 in DMF (3mL) (for ZIF-8-B and ZIF-8-B2) or methanol (3mL) (for ZIF-8-A2). ZIF-8-B was obtained by gentenly heating at 60ºC for 48h; ZIF-8-A2 was obtained by stirring for 48 at room temperature; ZIF-8-B2 was obtained by stirring for 48h at room temperature.For sensing measurements, the samples was exposured to saturated gases of H2S and the fluorescence changed was monitored

APRENDIZAGEM SIGNIFICATIVA: DESAFIOS DA AVALIAÇÃO NO ENSINO DE CIÊNCIAS

This article aims to discuss the challenges of evaluation in science education from a perspective of meaningful learning. This is part of an ongoing research, which has as its theme Science Teaching in Elementary School: A study on learning evaluation in the 5th grade of a Municipal School in Manaus/AM.  It is a bibliographic research with a qualitative approach, bringing authors such as Moreira (2017), Freire (2018/2020), Arroyo (2013), Fernandes (2014) among others. The reflections produced in this theoretical study point out the challenges present in the struggle movement, which cannot be limited to the narratives, and highlight the need to overcome the traditional logic of how students have been evaluated; the advancing in the construction of the evaluationas part of a significant learning process in science teaching, opening paths, possibilities, for new debates and dialogues in training spaces about evaluation.Este artículo tiene como objetivo discutir los desafíos de la evaluación en la educación científica desde la perspectiva del aprendizaje significativo. Se trata de una investigación en curso, cuyo tema es la Enseñanza de las Ciencias en la Escuela Primaria: un estudio sobre la evaluación del aprendizaje en el 5º grado de una Escuela Municipal de Manaus/AM. Se trata de una investigación bibliográfica con enfoque cualitativo, que reúne a autores como Moreira (2017), Freire (2018/2020), Arroyo (2013), Fernandes (2014) entre otros. Las reflexiones producidas en este estudio teórico apuntan a los desafíos presentes en el movimiento de lucha, que no pueden limitarse a las narrativas, y destacan la necesidad de superar la lógica tradicional de la evaluación de los estudiantes; también la construcción de la evaluación como parte de un proceso de aprendizaje significativo en la educación científica, abriendo caminos, posibilidades de nuevos debates y diálogos en espacios de formación sobre evaluación.O presente artigo tem como objetivo discutir os desafios da avaliação no ensino de ciências numa perspectiva de aprendizagem significativa. Trata-se do recorte de uma pesquisa em andamento, cujo tema é “Ensino de ciências: um estudo sobre a avaliação da aprendizagem no componente curricular de ciências, no 5º ano do ensino fundamental”. É um estudo bibliográfico com abordagem qualitativa, baseado nos autores Moreira (2017), Freire (2018/2020), Arroyo (2013), Fernandes (2014), dentre outros. As reflexões de caráter teórico, produzidas neste estudo, apontam os desafios presentes em movimentos de luta que não podem ficar apenas nas narrativas, e destacam a necessidade de superarmos a lógica tradicional de como os estudantes vêm sendo avaliados, a fim de caminhar na construção do processo de avaliação como parte de uma aprendizagem significativa no ensino de ciências, abrindo caminhos e possibilidades para novos debates e diálogos nos espaços de formação sobre a avaliação

Development and fine-tuning of an artificial organoleptic analysis method for virgin olive oil using an electronic olfactory system

Motivation: The catalogation of olive oil by organoleptic parameters has a high grade of subjection because of human factor. A new system of electronic tasting is used as a reliable, fast and cheap method. The use of an electronic olfatory system (EOS), which consist of an array of electonic sensors and a mechanism for pattern recognition, requires of a previous training process like a human olfatory system. The training is one of the most relevant and delicated phase, as this process involves the creation of a complete database that the instrument uses as a reference for the subsequent sample recognition.Methods: A set of samples of different olive oils were analysed by three official panel tests before using the EOS. The instrument is composed of a chamber with five metal oxide semiconductor based sensors. During the analysis, each sensor is maintained at a specific temperature showing a different response for a certain sample, which implies a reduction in the sensors resistance. For the measurement, volatile compounds were directed to the sensor chamber by the carrier gas (dry air). Principal component analyses to interpret the datasets from the EOS is employed in order to assess the classification of the measurements.A database was created through the analysis of a set of samples which should be representative of differents aromas to be recognized.Conclusions: The main conclussion is the high variability between the official panel tests which could be reduced by the EOS. From the database that was created we can analyse an olive oil with unknown catalogation and classify into Extra Virgin, virgin or lampante.On-line detection of the different categories, during the oil industrial process, could have a notable economical impact

Cachaça Classification Using Chemical Features and Computer Vision

Cacha¸ca is a type of distilled drink from sugarcane with great economic importance. Its classification includes three types: aged, premium and extra premium. These three classifications are related to the aging time of the drink in wooden casks. Besides the aging time, it is important to know what the wood used in the barrel storage in order the properties of each drink are properly informed consumer. This paper shows a method for automatic recognition of the type of wood and the aging time using information from a computer vision system and chemical information. Two algorithms for pattern recognition are used: artificial neural networks and k-NN (k-Nearest Neighbor). In the case study, 144 cacha¸ca samples were used. The results showed 97% accuracy for the problem of the aging time classification and 100% for the problem of woods classification.info:eu-repo/semantics/publishedVersio

Leucoplasias bucais: relação clínico-histopatológica

The relation between the clinical aspects and histologic characteristics of oral leukoplakias was evaluated on 28 adult patients presenting with oral leukoplakia. The lesions were clinically classified as homogeneous and nonhomogeneous, and divided regarding their histopathologic characteristics in six groups: hyperkeratosis without epithelial dysplasia; mild epithelial dysplasia; moderate epithelial dysplasia; severe epithelial dysplasia, squamous cell carcinoma in situ and invasive carcinoma. Homogeneous leukoplakia comprised 78.6% of the cases and nonhomogeneous, 21.4%. Microscopically, 32.2% of the lesions disclosed hyperkeratosis without evidences of epithelial dysplasia, 53.5% epithelial dysplasia (39.3% mild, 7.1% moderate, and 7.1% severe) and 14.3% invasive carcinoma. Homogeneous leukoplakia showed discreet histologic changes, while nonhomogeneous lesions evidenced histologic characteristics of severe epithelial dysplasia and even of invasive carcinoma. The results suggest great precaution in the diagnosis and follow-up of leukoplakia, considering the possibility of malignant transformation.A relação entre o aspecto clínico e as características histológicas das leucoplasias bucais foi avaliada em 28 pacientes adultos, que apresentaram lesões leucoplásicas na mucosa bucal. As lesões foram divididas quanto ao aspecto clínico em homogêneas e não-homogêneas, e classificadas segundo suas características histopatológicas em seis grupos: hiperceratose com ausência de displasia epitelial; displasia epitelial leve; displasia epitelial moderada; displasia epitelial severa, carcinoma in situ e carcinoma invasivo. Os resultados clínicos mostraram maior ocorrência de leucoplasias homogêneas (78,6%) do que não-homogêneas (21,4%). Os achados histopatológicos demonstraram que 32,2% dos casos apresentaram hiperceratose com ausência de displasia epitelial, 53,5% evidenciaram displasia epitelial (39,3% leve, 7,1% moderada e 7,1% severa) e 14,3% diagnosticados como carcinoma invasivo. As leucoplasias homogêneas apresentaram alterações celulares discretas, enquanto as não-homogêneas evidenciaram displasia epitelial severa e carcinoma invasivo. Os resultados sugerem um maior cuidado no diagnóstico e controle de leucoplasias dada a possibilidade de transformação maligna

Atividade Citotóxica do Extrato de Croton Urucurana Baill Contra linhagens de Células Leucêmicas Humanas U937 e THP 1

      Leukemias are malignant neoplasms that affect blood cells and originate in bone marrow. These are cancers of high incidence and are difficult to treat and there is a constant search for more efficient therapies. The present work investigated the cytotoxic action of the hydroalcoholic extract of Croton urucurana in human leukemia cells U937 and THP-1. The U937 and THP-1 cell lines were plated in a 100 μL/well volume (1x106 cells/mL) in 96-well plates, treated with extracts at final concentrations of 50 μg/mL, 100 μg/mL, 200 μg/mL and 400 μg/mL and 800 μg/mL  for cell viability tests with MTT(-3- (4.5-dimethyl-2-thiazole) 2.5-diphenyl-2H-tetrazolium bromide). After 48h, viability was assessed by MTT and the supernatant was used for determination of the Lactate Dehydrogenase (LD) enzyme. Fluorescence microscopy was used to evaluate apoptosis. The extract was able to reduce cell viability by the MTT assay, increase LD release and induce apoptosis in both cell lines tested at concentrations of 400 and 800 μg/ mL (p≤0.05). The C. urucurana extract presented cytotoxic activity against human leukemic cell lines U937 and THP-1, presenting itself as promising in the search for new antineoplastic agents.As leucemias são neoplasias malignas que acometem as células do sangue e tem origem na medula óssea. São cânceres de grande incidência e de difícil tratamento, havendo, portanto, a uma constante procura por terapias mais eficientes. O presente trabalho investigou a ação citotóxica do extrato hidroalcoólico de Croton urucurana em células leucêmicas humanas U937 e THP-1. As linhagens de células U937, THP-1 foram plaqueadas em volume de 100 μL/poço (1x106 cels/mL) em placas de 96 poços, tratadas com extrato nas concentrações finais de 50 μg/mL, 100 μg/mL, 200 μg/mL e 400 μg/mL e 800 μg/mL para os testes de viabilidade celular com MTT (-3-(4,5-dimetil-2-tiazol) 2,5-difenil-2-H-brometo de tetrazom). Após 48h a viabilidade foi avaliada pelo MTT e o sobrenadante utilizado para dosagem da enzima Lactato Desidrogenase (LD). Microscopia de fluorescência foi usada para avaliar a apoptose. O extrato foi capaz de reduzir a viabilidade celular pelo ensaio MTT, aumentar liberação da LD e de induzir apoptose em ambas as linhagens de células testadas nas concentrações 400 e 800 µg/mL (p≤0,05). O extrato de C. urucurana apresentou atividade citotóxica contra as linhagens de célula leucêmicas humanas U937 e THP-1, apresentando-se como promissor na busca por novos agentes antineoplásicos

Lipidomic signature of the green macroalgae Ulva rigida farmed in a sustainable integrated multi-trophic aquaculture

Ulva species, green macroalgae, are widely distributed across the globe, being one of the most heavily traded edible seaweeds. Nonetheless, although this genus has been largely used in scientific studies, its lipidome remains rather unexplored. The present study sheds light over the lipid profile of Ulva rigida produced in a land-based integrated multi-trophic aquaculture (IMTA) system using liquid chromatography coupled to high-resolution mass spectrometry for molecular lipid species identification. The lipidome of U. rigida revealed the presence of distinct beneficial n-3 fatty acids for human health, namely alpha-linoleic acid (ALA) and docosapentaenoic acid (DPA). A total of 87 molecular species of glycolipids, 58 molecular species of betaine lipids, and 57 molecular species of phospholipids were identified in the lipidome of U. rigida including some species bearing PUFA and with described bioactive properties. Overall, the present study contributes to the valorization and quality validation of sustainably farmed U. rigida.publishe