Music teacher as a teacher of choral speaking

Thesis (Ed.M.)--Boston University, 1941. This item was digitized by the Internet Archive

Molecular and comparative analysis of Salmonella enterica Senftenberg from humans and animals using PFGE, MLST and NARMS

<p>Abstract</p> <p>Background</p> <p><it>Salmonella </it>species are recognized worldwide as a significant cause of human and animal disease. In this study the molecular profiles and characteristics of <it>Salmonella enterica </it>Senftenberg isolated from human cases of illness and those recovered from healthy or diagnostic cases in animals were assessed. Included in the study was a comparison with our own sequenced strain of <it>S. </it>Senfteberg recovered from production turkeys in North Dakota. Isolates examined in this study were subjected to antimicrobial susceptibility profiling using the National Antimicrobial Resistance Monitoring System (NARMS) panel which tested susceptibility to 15 different antimicrobial agents. The molecular profiles of all isolates were determined using Pulsed Field Gel Electrophoresis (PFGE) and the sequence types of the strains were obtained using Multi-Locus Sequence Type (MLST) analysis based on amplification and sequence interrogation of seven housekeeping genes (<it>aroC</it>, <it>dnaN</it>, <it>hemD</it>, <it>hisD</it>, <it>purE</it>, <it>sucA</it>, and <it>thrA</it>). PFGE data was input into BioNumerics analysis software to generate a dendrogram of relatedness among the strains.</p> <p>Results</p> <p>The study found 93 profiles among 98 <it>S</it>. Senftenberg isolates tested and there were primarily two sequence types associated with humans and animals (ST185 and ST14) with overlap observed in all host types suggesting that the distribution of <it>S. </it>Senftenberg sequence types is not host dependent. Antimicrobial resistance was observed among the animal strains, however no resistance was detected in human isolates suggesting that animal husbandry has a significant influence on the selection and promotion of antimicrobial resistance.</p> <p>Conclusion</p> <p>The data demonstrates the circulation of at least two strain types in both animal and human health suggesting that <it>S. </it>Senftenberg is relatively homogeneous in its distribution. The data generated in this study could be used towards defining a pathotype for this serovar.</p

Evaluating corellations in Salmonella serotypes in swine in four longitudinal dataset

S. enterica serovars surveillance program have been established for many years in the United State of America (USA). Data from long running surveillance programs provides the opportunity to compare prevalence of serotypes within and across surveillance programs, observed patterns and generate hypothesis. To this end, the aim of this project was to estimate the correlation between changes in the yearly changes in serotypes proportions in concurrent years and lagged years from swine, beef and avian longitudinal datasets: (The Iowa State University Veterinary Diagnostic Laboratory (VDL), The National Antimicrobial Resistance Monitoring System ( NARMS animal-based isolates only), compared to data from The Centers for Disease Control (CDC) Laboratory-based Enteric Disease Surveillance (LEDS) Program. The lagged correlations were as follows: a) 1-year lag with the animal data preceding the human data and b) The correlation across a 2-year lag with the human data preceding the animal data

The efficacy of antibiotics to prevent collibacilosis in broiler poultry: A protocol for a systematic review and network meta-analysis.

Antibiotics are used in broiler poultry production both for the prevention and treatment of infectious diseases. However, antibiotic use is a driver of antibiotic resistance. The World Health Organization has published numerous reports urging all stakeholders concerned with both food-producing animals and humans to establish recommended steps to enhance the prudent use of antimicrobials (WHO, 2015). Similarly, the World Animal Health Organization has also published recommendations and position statements regarding prudent use and risk management related to antimicrobial use in animals (OIE, 2017). Colibacillosis is an important bacterial pathogen of poultry, and a costly disease for the industry resulting in multimillion dollar losses annually through morbidity, mortality or carcass condemnation at slaughter. Colibacillosis refers to any localized or systemic infection caused entirely or partly by the organism avian pathogenic Escherichia coli (APEC). These bacteria may be isolated as the sole pathogen or contribute to a disease complex with mixed viral and bacterial infections (Guabirara and Schouler, 2015). Two main disease processes important in the broiler industry are early mortality and cellulitis. Early mortality is defined by chicks under a week of age experiencing a higher than normal percentage of deaths in a flock. Early mortality can be caused by many things, for example chilling, overheating, or dehydration, however E.coli infection, or colibacillosis, is one of the main culprits. Colibacillosis can present with omphalitis, yolk sacculitis, enteritis, pasty vents, pericarditis, perihepatitis, polyserositis, congested lungs, splenomegaly and darkened proventriculus or any these combinations (Guabiraba and Schouler, 2015; Geetha and Palanivel, 2018). Many chicks succumb to an early and severe infection or are culled due to excessive morbidity. Antibiotics are typically used to reduce early mortality (Chauvin et al., 2005; Dziva and Stevens, 2008). Those with severe infection are unlikely to survive, however appropriate treatment reduces transmission between birds and improves the suitability of those with a mild infection. Not every labelled drug for E.coli is efficacious, resistance is common (Kabir, 2010) and effectiveness can vary from flock to flock, even within a flock, with more than one strain and more than one treatment. Understanding the efficacy of antibiotics used to prevent colibacillosis in broiler chickens is essential to optimizing their use; ineffective antibiotics should not be used for prevention or, if there are multiple efficacious antibiotics, their importance to human medicine should be considered when making decisions on antibiotic use. Systematic reviews of randomized controlled trials, and network meta-analysis to provide input on relative antibiotic efficacy, will yield the highest level of evidence for efficacy of treatments under field conditions (Sargeant and O’Connor, 2014)

Mechanisms of ER Stress-Mediated Mitochondrial Membrane Permeabilization.

During apoptosis, the process of mitochondrial outer membrane permeabilization (MOMP) represents a point-of-no-return as it commits the cell to death. Here we have assessed the role of caspases, Bcl-2 family members and the mitochondrial permeability transition pore on ER stress-induced MOMP and subsequent cell death. Induction of ER stress leads to upregulation of several genes such as Grp78, Edem1, Erp72, Atf4, Wars, Herp, p58ipk, and ERdj4 and leads to caspase activation, release of mitochondrial intermembrane proteins and dissipation of mitochondrial transmembrane potential (DeltaPsim). Mouse embryonic fibroblasts (MEFs) from caspase-9, -2 and, -3 knock-out mice were resistant to ER stress-induced apoptosis which correlated with decreased processing of pro-caspase-3 and -9. Furthermore, pretreatment of cells with caspase inhibitors (Boc-D.fmk and DEVD.fmk) attenuated ER stress-induced loss of DeltaPsim. However, only deficiency of caspase-9 and -2 could prevent ER stress-mediated loss of DeltaPsim. Bcl-2 overexpression or pretreatment of cells with the cell permeable BH4 domain (BH4-Tat) or the mitochondrial permeability transition pore inhibitors, bongkrekic acid or cyclosporine A, attenuated the ER stress-induced loss of DeltaPsim. These data suggest a role for caspase-9 and -2, Bcl-2 family members and the mitochondrial permeability transition pore in loss of mitochondrial membrane potential during ER stress-induced apoptosis

Spoilage Identification of Beef Using an Electronic Nose System

A commercially available Cyranose-320. conducting polymer-based electronic nose system was used to analyze the volatile organic compounds emanating from fresh beef strip loins (M. Longisimmus lumborum) stored at 4°C and 10°C. Two statistical techniques, i.e., linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA), were used to develop classification models from the collected sensor signals. The performances of the developed models were validated by two different methods: leave-1-out cross-validation, and bootstrapping. The developed models classified meat samples based on the microbial population into “unspoiled” (microbial counts \u3c6.0 log10 cfu/g) and “spoiled” (microbial counts \u3e 6.0 log10 cfu/g). Overall, quadratic discriminant-based classification models performed better than linear discriminant analysis based models. For the meat samples stored at 10°C, the highest classification accuracies obtained by the LDA method with leave-1-out and bootstrapping validations were 87.10% and 85.87%, respectively. On the other hand, classification by QDA and subsequent validation by leave-1-out and bootstrapping provided highest accuracies of 87.5% and 97.38%, respectively. For samples stored at 4°C, the LDA method provided highest classification accuracies of 79.17% and 85.64% using leave-1-out and bootstrapping validation, respectively. When the QDA method was used, the highest classification accuracies obtained for the samples stored at 4°C were 87.50% and 98.48%, respectively, with leave-1-out and bootstrapping validations

Escherichia coli, Salmonella, and Mycobacterium avium subsp. paratuberculosis in Wild European Starlings at a Kansas Cattle Feedlot

The prevalence of Escherichia coli, Salmonella spp., and Mycobacterium avium subsp.paratuberculosis isolated from the feces of wild European starlings (Sturnus vulgaris) humanely trapped at a feedlot in central Kansas was assessed. All E. coli and Salmonellaisolates recovered were tested for antimicrobial susceptibility using National Antimicrobial Resistance Monitoring System panels and the E. coli isolates were classified as to their content of genes associated with pathogenic E. coli of birds and cattle, including cvaC, iroN2, ompTp, hlyF2, eitC, iss, iutA, ireA, papC, stxI, stxII, sta, K99, F41, and eae.Escherichia coli O157:H7 and Mycobacterium avium subsp. paratuberculosis were not detected and Salmonella was isolated from only three samples, two of which displayed antimicrobial resistance. Approximately half of the E. coli isolates were resistant to antimicrobial agents with 96% showing resistance to tetracycline. Only one isolate was positive for a single gene associated with bovine pathogenic E. coli. An interesting finding of this study was that 5% of the E. coli isolates tested met the criteria established for identification as avian pathogenic E. coli (APEC). Thus these findings suggest that starlings are not a significant source of Salmonella spp., Mycobacterium avium subsp.paratuberculosis, E. coli O157, or other shiga toxin–producing E. coli in this feedlot. However, they may have the potential to spread APEC, an important pathogen of poultry and a potential pathogen to human beings