Privacy-preserving Distributed Machine Learning via Local Randomization and ADMM Perturbation

With the proliferation of training data, distributed machine learning (DML) is becoming more competent for large-scale learning tasks. However, privacy concerns have to be given priority in DML, since training data may contain sensitive information of users. In this paper, we propose a privacy-preserving ADMM-based DML framework with two novel features: First, we remove the assumption commonly made in the literature that the users trust the server collecting their data. Second, the framework provides heterogeneous privacy for users depending on data's sensitive levels and servers' trust degrees. The challenging issue is to keep the accumulation of privacy losses over ADMM iterations minimal. In the proposed framework, a local randomization approach, which is differentially private, is adopted to provide users with self-controlled privacy guarantee for the most sensitive information. Further, the ADMM algorithm is perturbed through a combined noise-adding method, which simultaneously preserves privacy for users' less sensitive information and strengthens the privacy protection of the most sensitive information. We provide detailed analyses on the performance of the trained model according to its generalization error. Finally, we conduct extensive experiments using real-world datasets to validate the theoretical results and evaluate the classification performance of the proposed framework

Regulation of gene expression by FSP27 in white and brown adipose tissue

<p>Abstract</p> <p>Background</p> <p>Brown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT and WAT and is associated with lipid droplets. Over-expression of FSP27 promotes lipid storage, whereas <it>FSP27 </it>deficient mice have improved insulin sensitivity and are resistant to diet-induced obesity. In addition, <it>FSP27</it>-deficient white adipocytes have reduced lipid storage, smaller lipid droplets, increased mitochondrial activity and a higher expression of several BAT-selective genes. To elucidate the molecular mechanism by which FSP27 controls lipid storage and gene expression in WAT and BAT, we systematically analyzed the gene expression profile of <it>FSP27-</it>deficient WAT by microarray analysis and compared the expression levels of a specific set of genes in WAT and BAT by semi-quantitative real-time PCR analysis.</p> <p>Results</p> <p>BAT-selective genes were significantly up-regulated, whereas WAT-selective genes were down-regulated in the WAT of <it>FSP27-</it>deficient mice. The expression of the BAT-selective genes was also dramatically up-regulated in the WAT of <it>leptin/FSP27 </it>double deficient mice. In addition, the expression levels of genes involved in multiple metabolic pathways, including oxidative phosphorylation, the TCA cycle, fatty acid synthesis and fatty acid oxidation, were increased in the <it>FSP27-</it>deficient WAT. In contrast, the expression levels for genes involved in extracellular matrix remodeling, the classic complement pathway and TGF-β signaling were down-regulated in the <it>FSP27-</it>deficient WAT. Most importantly, the expression levels of regulatory factors that determine BAT identity, such as CEBPα/β, PRDM16 and major components of the cAMP pathway, were markedly up-regulated in the WAT of <it>FSP27-</it>deficient mice. The expression levels of these regulatory factors were also up-regulated in <it>leptin/FSP27 </it>double deficient mice. Interestingly, distinct gene expression profiles were observed in the BAT of <it>FSP27-</it>deficient mice. Taken together, these data suggest that the WAT of <it>FSP27-</it>deficient mice have a gene expression profile similar to that of BAT.</p> <p>Conclusions</p> <p>FSP27 acts as a molecular determinant that controls gene expression for a diversity of metabolic and signaling pathways and, in particular, the expression of regulatory factors, including CEBPα/β, PRDM16 and components of the cAMP signaling pathway, that control the identity of WAT and BAT.</p

Effect of Sow Intestinal Flora on the Formation of Endometritis

Endometritis is the main cause of decreased reproductive performance of sows, while one of the most important factors in the etiology of sow endometritis is an aberration of birth canal microbiota. Therefore, people began to pay attention to the microbiota structure and composition of the birth canal of sows with endometritis. Interestingly, we found that the risk of endometritis was increased in the sows with constipation in clinical practice, which may imply that the intestinal flora is related to the occurrence of endometritis. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceptionally crucial. In this study, the microbiota of birth canal secretions and fresh feces of four healthy and four endometritis sows were analyzed via sequencing the V3 + V4 region of bacterial 16S ribosomal (rDNA) gene. The results showed a significant difference between endometritis and healthy sows birth canal flora in composition and abundance. Firmicutes (74.36%) and Proteobacteria were the most dominant phyla in birth canal microbiota of healthy sows. However, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus) declined in endometritis sow. The abundance of Porphyromonas, Clostridium sensu stricto 1, Streptococcus, Fusobacterium, Actinobacillus, and Bacteroides increased significantly in the birth canal microbiota of endometritis sows. Escherichia–Shigella and Bacteroides were the common genera in the birth canal and intestinal flora of endometritis sows. The abundance of Escherichia–Shigella and Bacteroides in the intestines of sows suffering from endometritis were significantly increased than the intestinal microbiota of the healthy sows. We speculated that some intestinal bacteria (such as Escherichia–Shigella and Bacteroides) might be bound up with the onset of sow endometritis based on intestinal microbiota analysis in sows with endometritis and healthy sows. The above results can supply a theoretical basis to research the pathogenesis of endometritis and help others understand the relationship with the microbiota of sow's birth canal and gut

The multidrug-resistant Pseudomonas fluorescens strain: a hidden threat in boar semen preservation

Although the bacterial composition of boar ejaculate has been extensively studied, the bacterial composition of extended boar semen is often overlooked, despite the potential risks these microorganisms may pose to the long-term preservation of extended boar semen at 15–17°C. In this study, we characterized the bacterial community composition of extended semen and discovered that Pseudomonas spp. was the dominant flora. The dominant strains were further isolated and identified as a potential new species in the Pseudomonas fluorescens group and named GXZC strain, which had adverse effects on sperm quality and was better adapted to growth at 17°C. Antimicrobial susceptibility testing showed that the GXZC strain was resistant to all commonly used veterinary antibiotics. Whole-genome sequencing (WGS) and genome annotation revealed the large genetic structure and function [7,253,751 base pairs and 6,790 coding sequences (CDSs)]. Comparative genomic analysis with the closest type strains showed that the GXZC strain predicted more diversity of intrinsic and acquired resistance genes to multi-antimicrobial agents. Taken together, our study highlights a problem associated with the long-term storage of extended boar semen caused by a P. fluorescens group strain with unique biological characteristics. It is essential to develop a new antibacterial solution for the long-term preservation of boar semen

LRRK2 mediated Rab8a phosphorylation promotes lipid storage

Abstract Background Several mutations in leucine rich repeat kinase 2 (LRRK2) gene have been associated with pathogenesis of Parkinson’s disease (PD), a neurodegenerative disorder marked by resting tremors, and rigidity, leading to Postural instability. It has been revealed that mutations that lead to an increase of kinase activity of LRRK2 protein are significantly associated with PD pathogenesis. Recent studies have shown that some Rab GTPases, especially Rab8, serve as substrates of LRRK2 and undergo phosphorylation in its switch II domain upon interaction. Current study was performed in order to find out the effects of the phosphorylation of Rab8 and its mutants on lipid metabolism and lipid droplets growth. Methods The phosphorylation status of Rab8a was checked by phos-tag gel. Point mutant construct were generated to investigate the function of Rab8a. 3T3L1 cells were transfected with indicated plasmids and the lipid droplets were stained with Bodipy. Fluorescent microscopy experiments were performed to examine the sizes of lipid droplets. The interactions between Rab8a and Optineurin were determined by immunoprecipitation and western blot. Results Our assays demonstrated that Rab8a was phosphorylated by mutated LRRK2 that exhibits high kinase activity. Phosphorylation of Rab8a on amino acid residue T72 promoted the formation of large lipid droplets. T72D mutant of Rab8a had higher activity to promote the formation of large lipid droplets compared with wild type Rab8a, with increase in average diameter of lipid droplets from 2.10 μm to 2.46 μm. Moreover, phosphorylation of Rab8a weakened the interaction with its effector Optineurin. Conclusions Y1699C mutated LRRK2 was able to phosphorylate Rab8a and phosphorylation of Rab8a on site 72 plays important role in the fusion and enlargement of lipid droplets. Taken together, our study suggests an indirect relationship between enhanced lipid storage capacity and PD pathogenesis

Impact of the COVID-19 Pandemic on Physical and Mental Health in Lower and Upper Middle-Income Asian Countries: A Comparison Between the Philippines and China

10.3389/fpsyt.2020.568929Frontiers in Psychiatry1156892

A longitudinal study on the mental health of general population during the COVID-19 epidemic in China

10.1016/j.bbi.2020.04.028BRAIN BEHAVIOR AND IMMUNITY8740-4

Mental Health of the General Population during the 2019 Coronavirus Disease (COVID-19) Pandemic: A Tale of Two Developing Countries

Background: This study aimed to compare the severity of psychological impact, anxiety and depression between people from two developing countries, Iran and China, and to correlate mental health parameters with variables relating to the COVID-19 pandemic. Although China and Iran are developing countries based on the World Bank’s criteria, these two countries are different in access to resources and health care systems. We hypothesized that Iranians would show higher levels of depression, anxiety and stress as compared to Chinese. Methods: This study collected information related to the COVID-19 pandemic including physical health, precautionary measures and knowledge about the pandemic. We also used validated questionnaires such as the Impact of Event Scale-Revised (IES-R) and the Depression, Anxiety and Stress Scale (DASS-21) to assess the mental health status. Results: There were a total of 1411 respondents (550 from Iran; 861 from China). The mean IES-R scores of respondents from both countries were above the cut-off for post-traumatic stress disorder (PTSD) symptoms. Iranians had significantly higher levels of anxiety and depression (p &lt; 0.01). Significantly more Iranians believed COVID-19 was transmitted via contact, practised hand hygiene, were unsatisfied with health information and expressed less confidence in their doctors, but were less likely to wear a facemask (p &lt; 0.001). Significantly more Iranians received health information related to COVID-19 via television while Chinese preferred the Internet (p &lt; 0.001). Conclusions: This cross-country study found that Iranians had significantly higher levels of anxiety and depression as compared to Chinese. The difference in reported measures between respondents from Iran and China were due to differences in access to healthcare services and governments’ responses to the pandemic