Temporomandibular Joint Pathology and Its Indication in Clinical Orthodontics

Temporomandibular joint (TMJ) pathology has been an area of study in dentistry specifically a research interest in clinical orthodontics in which treatment option has been a dilemma for practitioners. Discussion between ‘dos’ and ‘don’ts’ in growth modification has drawn spread however opposite opinions from different schools of thoughts in whether growth modification itself is working. To provide a better illustration of biological process within TMJ, this chapter discussed aspects including overall condylar growth; the histological structure of endochondral bone of condyle; extracellular factors that regulate proliferation, differentiation, hypertrophy, terminal maturation and apoptosis of chondrocytes; and molecular regulation of the entire process. An understanding of the pathology, histology, cellular and molecular events related to the morphology and growth of TMJ forms through reading over this chapter; the emphasis of the mechanotransduction mediators and the influence of mechanical strain on the level of expression of genes were presented in details. Novel studies using virus vector stimulating condylar growth through enhancing angiogenesis within a time limit were discussed; also clinical implications in treatment options in relation to mandibular advancement were briefly compared

Generation of mice with a conditional allele for the p75 NTR neurotrophin receptor gene

The p75 NTR neurotrophin receptor has been implicated in multiple biological and pathological processes. While significant advances have recently been made in understanding the physiologic role of p75 NTR , many details and aspects remain to be determined. This is in part because the two existing knockout mouse models (Exons 3 or 4 deleted, respectively), both display features that defy definitive conclusions. Here we describe the generation of mice that carry a conditional p75 NTR (p75 NTR‐FX ) allele made by flanking Exons 4–6, which encode the transmembrane and all cytoplasmic domains, by loxP sites. To validate this novel conditional allele, both neural crest‐specific p75 NTR /Wnt1‐Cre mutants and conventional p75 NTR null mutants were generated. Both mutants displayed abnormal hind limb reflexes, implying that loss of p75 NTR in neural crest‐derived cells causes a peripheral neuropathy similar to that seen in conventional p75 NTR mutants. This novel conditional p75 NTR allele will offer new opportunities to investigate the role of p75 NTR in specific tissues and cells. genesis 49:862–869, 2011. © 2011 Wiley‐Periodicals, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88029/1/20747_ftp.pd

Rice Soluble Starch Synthase I: Allelic Variation, Expression, Function, and Interaction With Waxy

Starch, which is composed of amylose and amylopectin, is the key determinant of rice quality. Amylose is regulated by the Waxy (Wx) gene, whereas amylopectin is coordinated by various enzymes including eight soluble starch synthases (SSSs), of which SSSI accounts for ∼70% of the total SSS activity in cereal endosperm. Although great progress has been made in understanding SSSI gene expression and function, allelic variation and its effects on gene expression, rice physicochemical properties and qualities, and interactions with the Wx gene remain unclear. Herein, SSSI nucleotide polymorphisms were analyzed in 165 rice varieties using five distinct molecular markers, three of which reside in an SSSI promoter and might account for a higher expression of the SSSIi allele in indica ssp. than of the SSSIj allele in japonica ssp. The results of SSSI promoter-Beta-Glucuronidase (β-GUS) analysis were consistent with the expression results. Moreover, analysis of near isogenic lines (NILs) in the Nipponbare (Nip) background showed that Nip (SSSIi) and Nip (SSSIj) differed in their thermal properties, gel consistency (GC), and granule crystal structure. Knockdown of SSSI expression using the SSSI-RNA interference (RNAi) construct in both japonica and indica backgrounds caused consistent changes in most tested physicochemical characteristics except GC. Moreover, taste value analysis (TVA) showed that introduction of the SSSI allele in indica or knockdown of SSSI expression in japonica cultivars significantly reduced the comprehensive taste value, which was consistent with the superior taste of japonica against indica. Furthermore, to test the potential interaction between SSSI and different Wx alleles, three NILs within the Wx locus were generated in the indica cv. Longtefu (LTF) background, which were designated as LTF (Wxa), LTF (Wxb), and LTF (wx). The SSSI-RNAi construct was also introduced into these three NILs, and physiochemical analysis confirmed that the knockdown of SSSI significantly increased the rice apparent amylose content (AAC) only in the Wxa and Wxb background and caused different changes in GC in the NILs. Therefore, the effect of SSSI variation on rice quality also depends on its crosstalk with other factors, especially the Wx gene. These findings provide fundamental knowledge for future breeding of rice with premium eating and cooking qualities

A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics.

BACKGROUND: Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China. METHODS: Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated, in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test. RESULTS: A phage able to lyse 24/25 clinical isolates was recovered. It was classified in the Podoviridae family and was found to be amenable to propagation. Under the optimal multiplicity of infection (MOI) of 0.1, an eclipse period of around 20 min and a high titer (1012 PFU/ml) produced within 1 h were demonstrated. This phage was found stabile at a wide range of temperatures (24, 37, 40, 50, and 60 °C) and pH values (3-12). After being designated as vB_BpP_HN01, it was fully sequenced, and the 71,398 bp linear genome, containing 93 open reading frames and a tRNA-Asn, displayed a low sequence similarity with known viruses. Additionally, protective effects of applications of vB_BpP_HN01 (MOI = 0.1 and MOI = 1) alone or in combination with antibiotics were found to improve viability of infected cells (70.6 ± 6.8%, 85.8 ± 5.7%, 91.9 ± 1.8%, and 96.8 ± 1.8%, respectively). A significantly reduced mortality (10%) and a decreased pathogen load were demonstrated in infected C. elegans following the addition of this phage. CONCLUSIONS: As the first B. pseudomallei phage was isolated in Hainan, China, phage vB_BpP_HN01 was characterized by promising lytic property, stability, and efficiency of bacterial elimination during the in vitro/vivo experiments. Therefore, we can conclude that it is a potential alternative agent for combating melioidosis

Vastatin, a novel angiogenesis inhibitor, retards condylar bone growthin vivo

published_or_final_versionDentistryDoctoralDoctor of Philosoph

The influence of African swine fever information on consumers’ preference of pork attributes and pork purchase

This paper uses a randomized survey instrument to study the impact of African Swine Fever (ASF) information on Chinese consumers’ preference for pork attributes and purchases during the recent peak of the ASF pandemic in 2019. We study consumers’ preference for pork attributes including brand, meat texture and taste, quality safety assurance, and traceability under different information treatments. Results show that the willingness to pay (WTP) for quality safety assurance is the highest, followed by brands and traceability systems, and the WTP is lowest for good taste. We show that providing detailed ASF information substantially changes consumer preference by altering the relative importance of pork attributes and price sensitivity, which enables consumers to focus more on safety-related attributes while paying less attention to price and taste attributes. Furthermore, we find that a higher belief in the future of ASF occurrence reduces the frequency of purchases marginally but does not significantly influence for amount per purchase and the total purchase amount

Thermodynamic analysis of hydrocarbon reforming

A holistic hydrocarbon reforming analysis is performed using equilibrium calculations based on Gibbs energy minimization. The reforming feed composition is defined based on the oxygen to carbon atom ratio and the hydrogen to carbon atom ratio. The coke formation, carbon dioxide yield, and synthesis gas mole fraction were investigated to determine an operating composition region for hydrocarbon reforming while the hydrogen yield, hydrogen to carbon monoxide ratio, and the stoichiometric ratio were investigated to determine the reforming feed composition to produce hydrogen and optimal synthesis gas compositions for downstream processes. Our findings show that the synthesis of hydrogen via the reforming process will result in carbon dioxide emissions for any hydrocarbon feed. To produce the optimal synthesis gas for oxo-synthesis, Fischer-Tropsch synthesis and methanol synthesis, our findings show that reforming with methane provides the best opportunity for incorporation of carbon dioxide, while the reforming of coke does not result in the incorporation of carbon dioxide.•Reforming feedstock is characterized by H/C and O/C ratios.•Reforming saturated non-oxygenated hydrocarbons yield highest syn gas mole fraction.•Reforming of methane has highest potential for incorporating CO2 into synthesis gas.•Reforming of coke to synthesis gas with H2/CO ratio > 1 result in CO2 emissions.•H2 production from hydrocarbon reforming processes will result in CO2 emissions