Propuesta en Supply Chain Management y Logística en la empresa RENAL MEDICAL

No aplicaEl presente trabajo es realizado por el grupo 57 del curso Diplomado en SCM y Logística O.G. 1601 del 2021, con el fin de realizar una introspección a todos los aspectos de la gestión de la cadena de suministro de Renal Medical Marketing Limitada. Se pretende denotar el análisis realizado a los procesos actuales y a todas las áreas de la compañía, así mismo aprovechar la disposición de Renal Medical Marketing Limitada fabricante, importadora y distribuidora de dispositivos médicos en cuento la generación del espacio para el desarrollo del diplomado de supply chain.No aplic

Systematic Roadmap for Cancer Drug Screening Using Zebrafish Embryo Xenograft Cancer Models: Melanoma Cell Line as a Case Study

From MDPI via Jisc Publications RouterHistory: accepted 2021-07-19, pub-electronic 2021-07-23Publication status: PublishedFunder: Gobierno de Navarra; Grant(s): 0011-1408-2016-000004, 0011-1365-2020-000292Zebrafish embryo tumor transplant models are widely utilized in cancer research. Compared with traditional murine models, the small size and transparency of zebrafish embryos combined with large clutch sizes that increase statistical power and cheap husbandry make them a cost-effective and versatile tool for in vivo drug discovery. However, the lack of a comprehensive analysis of key factors impacting the successful use of these models impedes the establishment of basic guidelines for systematic screening campaigns. Thus, we explored the following crucial factors: (i) user-independent inclusion criteria, focusing on sample homogeneity; (ii) metric definition for data analysis; (iii) tumor engraftment criteria; (iv) image analysis versus quantification of human cancer cells using qPCR (RNA and gDNA); (v) tumor implantation sites; (vi) compound distribution (intratumoral administration versus alternative inoculation sites); and (vii) efficacy (intratumoral microinjection versus compound solution in media). Based on these analyses and corresponding assessments, we propose the first roadmap for systematic drug discovery screening in zebrafish xenograft cancer models using a melanoma cell line as a case study. This study aims to help the wider cancer research community to consider the adoption of this versatile model for cancer drug screening projects

Bactericidal synergism between antibiotics and phage endolysin Cpl-711 to kill multidrug-resistant pneumococcus

Aim: To test the synergistic effect of Cpl-711 endolysin and antibiotics for antipneumococcal activity. Materials & methods: A combination of Cpl-711 and different antibiotics (amoxicillin, cefotaxime, levofloxacin and vancomycin) was tested in a checkerboard assay against several multidrug-resistant Streptococcus pneumoniae strains. Mouse and zebrafish models of pneumococcal sepsis were used to confirm the in vitro data. Results: The activity of Cpl-711 combined with amoxicillin or cefotaxime was synergistic in the bactericidal effect against a serotype 23F multiresistant clinical isolate of S. pneumoniae. Synergy between Cpl-711 and cefotaxime was validated using both mouse and zebrafish models. Conclusion: Combination of Cpl-711 and cefotaxime may help in the treatment of diseases caused by multiresistant pneumococcal strains.This research was supported by grants from the Ministerio de Economía y Competitividad (MINECO) to P García (SAF2017-88664) and JE Yuste (SAF2017-83388). Additional funding was provided by the CIBER de Enfermedades Respiratorias (CIBERES), an initiative of the Instituto de Salud Carlos III (ISCIII).Peer Reviewe

Systematic Roadmap for Cancer Drug Screening Using Zebrafish Embryo Xenograft Cancer Models: Melanoma Cell Line as a Case Study

Zebrafish embryo tumor transplant models are widely utilized in cancer research. Compared with traditional murine models, the small size and transparency of zebrafish embryos combined with large clutch sizes that increase statistical power and cheap husbandry make them a cost-effective and versatile tool for in vivo drug discovery. However, the lack of a comprehensive analysis of key factors impacting the successful use of these models impedes the establishment of basic guidelines for systematic screening campaigns. Thus, we explored the following crucial factors: (i) user-independent inclusion criteria, focusing on sample homogeneity; (ii) metric definition for data analysis; (iii) tumor engraftment criteria; (iv) image analysis versus quantification of human cancer cells using qPCR (RNA and gDNA); (v) tumor implantation sites; (vi) compound distribution (intratumoral administration versus alternative inoculation sites); and (vii) efficacy (intratumoral microinjection versus compound solution in media). Based on these analyses and corresponding assessments, we propose the first roadmap for systematic drug discovery screening in zebrafish xenograft cancer models using a melanoma cell line as a case study. This study aims to help the wider cancer research community to consider the adoption of this versatile model for cancer drug screening projects

Phage lysins against pneumococcal colonization

35 p.-7 fig.-1 tab.Bacteriophage-encoded lytic enzymes, also named lysins, or enzybiotics, are efficient agents to kill bacterial pathogens. Colonization of the respiratory tract by Streptococcus pneumoniae is a prerequisite for the establishment of the infection process. Hence, we have evaluated the antibacterial activity of three different lysins against pneumococcal colonization using human nasopharyngeal and lung epithelial cells as well as a mouse model of nasopharyngeal colonization. The lysins tested were the wild type Cpl-1, the engineered Cpl-7S, and the chimera Cpl-711. Moreover, we have included amoxicillin as a comparator antibiotic. Human epithelial cells were infected with three different multidrug-resistant clinical isolates of S. pneumoniae followed by a single dose of the corresponding lysin. The antimicrobial activity of these lysins was also evaluated using a mouse nasopharyngeal carriage model. Exposure of infected epithelial cells to Cpl-7S did not result in the killing of any of the pneumococcal strains investigated. However, treatment with Cpl-1 or Cpl-711 increased the killing of S. pneumoniae adhered to both types of human epithelial cells with Cpl-711 being more effective than Cpl-1, at sub-inhibitory concentrations. In addition, treatment with amoxicillin had no effect reducing the carrier state whereas mice treated by the intranasal route with Cpl-711 showed significantly reduced nasopharyngeal colonization with no detection of bacterial load in 20-40% of the mice. This study indicates that Cpl-1 and Cpl-711 lysins might be promising antimicrobial candidates for therapy against pneumococcal colonization.This work was supported by grants SAF2017-83388 and SAF2017-88664 from Ministerio de Economía y Competitividad (MINECO)Peer reviewe