Erratum: Surveillance de l'endometre sous traitement hormonal substitutif par echo-Doppler (Journal d'Echographie et de Medecine par Ultrasons)

SCOPUS: er.jinfo:eu-repo/semantics/publishe

Identification and characterization of α1-adrenergic receptors in human myometrium by [3H]prazosin binding

α1-adrenergic receptors can be directly characterized in myometrial membranes using the specific α1-adrenergic radioligand [3H]prazosin. Compared to the previous approach, this allows a more precise determination of the α1-adrenergic receptor number and of their agonist and antagonist binding properties. Use of this radioligand might prove to be a powerful tool in distinguishing α1-receptors from the total amount of α-adrenergic receptors. As a practical application, it becomes now possible to determine whether the reported influence of sex steroid hormones on the total α-adrenergic receptor population in animal uteri does also apply to the α1-subpopulation.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Selective versus universal screening for gestational diabetes mellitus: an evaluation of Naylor's model.

Selective screening for gestational diabetes is still a subject of debate. The aim of this study was to determine the effectiveness of a selective screening strategy for diagnosing gestational diabetes in our setting. This strategy rests on a scoring system that assigns women to different risk categories according to the presence of risk factors such as overweight, older age and ethnicity.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

A single cervical fetal fibronectin screening test in a population at low risk for preterm delivery: An improvement on clinical indicators?

Objective To assess the accuracy of a single cervical fetal fibronectin test to predict spontaneous preterm delivery in an unselected antenatal population. Design A prospective blind cohort study. Setting Antenatal clinic of a teaching hospital in a Brussels semiurban area. Participants An unselected group of 170 women followed at the antenatal clinic. Methods A single cervical sample was obtained between 24 and 33 completed weeks of pregnancy. The fibronectin test was compared with clinical evaluation and their predictive properties were assessed. Results Fifteen women were excluded from the analysis because of elective preterm delivery for medical indications or loss to follow up. Of the 155 remaining women, nine (7%) had a spontaneous preterm delivery. For a single fetal fibronectin test, the sensitivity was 26.7%, the specificity 95.7%, and the positive and negative predictive values 40.0% and 92.4%, respectively. The likelihood ratio of a positive was similar to that of clinical predictors of preterm birth (LR = 6.2; 95% CI 2.0-19.6). Sensitivities were low for both clinical criteria and the fetal fibronectin test. Conclusions Because of low sensitivity in a low risk population, screening for preterm delivery should not be based on the result of a single fetal fibronectin test alone. However, due to its high specificity the test might be useful in avoiding unnecessary medical intervention.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Surveillance de l'endometre sous traitement hormonal de substitution par echo-Doppler

Objectives: To determine endometrial thickness, uterine and endometrial blood flow in postmenopausal women receiving hormone replacement therapy (HRT). Study design: forty hundred forty eight women were divided into six groups based on HRT. The endometrial thickness of all of them were examinated with Aloka 2000 and pulsatility and resistance index of uterine and endometrial arteries (if visible) were calculated. P < 0.05 was considered as significative. Result: The endometrial thickness was 2.8 mm ± 2.6 in group without HRT (n = 168), this endometrial thickness was significatively (p < 0.0001) lower than group with oestrogen alone: 8.7 mm ± 6, HRT sequential: 6.7 mm ± 3, HRT continous: 4 mm ± 3.1 (p<0.01), Progestagen alone: 8 mm ± 5.8 and Tibolone 6.7 mm ± 4. The mean RIs and PIs of uterine arteries are significantly lower in women receiving HRT (group 2-6) than women without HRT (0.72 ± 0.12 versus 0.92 ± 0.01) p < 0.01 and 2.16 ± 0.8 versus 2.46 ± 0.8 p < 0.01. The endometrial PI of 1.17 ± 0.4 in the HRT group was not significantly different from that in the no HRT group (1.85 ± 0.4) p = 0.06. Conclusion: The endometrial thickness was considerably lower with continous hormone replacement therapy around 4 mm. Moderate endometrial blood flow were present in all groups. The color doppler ultrasound reflects the hormonal status and change in the uterine and endometrial perfusion depending on hormonal therapy.SCOPUS: re.jinfo:eu-repo/semantics/publishe

Doppler couleur energie au cours du cycle menstruel

Doppler ultrasonography has been used over the last few years to investigate uteroovarian hemodynamics during the menstrual cycle in women and in nonhuman primates. In the present study, pulsed and energy Doppler imaging was used to determine the PI and RI values of the arcuate, radial, and endometrial uterine arteries and ovarian stroma on days 9, 12, 15, 23, and 26 of the menstrual cycle in ten young volunteers. Cycles were classified as ovulatory (n = 7) or nonovulatory (n = 3) based on the temperature curve and on whether a corpus luteum was visible upon ultrasonography. PI and RI values of the arcuate and radial uterine arteries on the dominant side were significantly lower during the luteal phase than during the follicular phase (P < 0.05). Only during ovulatory cycles were the endometrial arteries visualized during the luteal phase. Thickness of the endometrium was positively correlated with endometrial PI and RI values (r = 0.47; P < 0.01). Perifollicular flows were visualized on day 9 during both ovulatory and nonovulatory cycles. No significant differences were found in perifollicular PI and RI values between the dominant side and the nondominant side; neither were significant differences found in anovulatory cycle PI and RI values between the right side and the left side. PI values for ovarian stroma arteries on the side of ovulation decreased throughout the cycle, from 1.36 ± 0.28 on D9 to 1.25 ± 0.82 on D23 and 1 ± 0.21 on D26; the decrease was less marked on the opposite side. Pulsed and energy Doppler imaging demonstrates endometrial vascularization and increased blood flows in the ovarian stroma and corpus luteum indicating better perfusion during ovulatory cycles.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Myometrial β1-adrenoreptors are detectable only in the midfollicular phase

Both α- and β-adrenergic receptors have been identified in human myometrium by radioligand binding. Both types of receptors mediate uterine contractility: α-adrenergic agonists cause uterine contraction, whereas β-adrenergic agonists induce relaxation. We studied the possible regulatory effects of gonadal steroids on the affinity, concentration, subtype distribution, and linkage to adenylate cyclase of β-adrenergic receptors in human myometrium removed during different phases of the menstrual cycle, from postmenopausal women and during depo-progestin (medroxyprogesterone acetate) therapy. We identified β-adrenergic receptors in human myometrial membranes using the radiolabeled antagonist (-)-[3H]-dihydroalprenolol (DHA). The binding of this radioligand was rapid, reversible, of high affinity (K(D)=0.71 nM) and stereoselective. Total β-receptor concentration was determined by Scatchard analysis of DHA saturation binding and the ratio of receptor subtypes determined by computer-assisted analysis of β2 selective antagonist ICI 118 551/DHA competition binding curves. The fraction of receptors functionally coupled to adenylate cyclase was determined by the agonist/N-ethylmaleimide inactivation method. The affinity of DHA and the fraction of receptors undergoing functional coupling was similar under all hormonal conditions. However, whereas the net concentration of β-receptors was the same in all groups, β1-adrenoreceptors could only be detected in myometrial particulate fractions from uteri obtained in the midfollicular phase, indicating the importance of considering adrenoceptor subtypes as separately regulatable receptors.SCOPUS: ar.jinfo:eu-repo/semantics/publishe