Harnessing the power of cell transplantation to target respiratory dysfunction following spinal cord injury.

The therapeutic benefit of cell transplantation has been assessed in a host of central nervous system (CNS) diseases, including disorders of the spinal cord such as traumatic spinal cord injury (SCI). The promise of cell transplantation to preserve and/or restore normal function can be aimed at a variety of therapeutic mechanisms, including replacement of lost or damaged CNS cell types, promotion of axonal regeneration or sprouting, neuroprotection, immune response modulation, and delivery of gene products such as neurotrophic factors, amongst other possibilities. Despite significant work in the field of transplantation in models of SCI, limited attention has been directed at harnessing the therapeutic potential of cell grafting for preserving respiratory function after SCI, despite the critical role pulmonary compromise plays in patient outcome in this devastating disease. Here, we will review the limited number of studies that have demonstrated the therapeutic potential of intraspinal transplantation of a variety of cell types for addressing respiratory dysfunction in SCI

An inexpensive and continuous radon progeny detector for indoor air-quality monitoring

A silicon photodiode-based inexpensive detector working as a counter and spectrometer for alpha particles has been conceived, designed, constructed and analyzed in depth. Monte Carlo simulations by means of MCNPX ver. 2.7.0 code have been carried out to select the most suitable sensitive element for the intended applications. The detecting unit has been coupled to an Arduino board and tested for low-rate alpha-particle counting and spectroscopy. Results demonstrate a maximum count rate of 4000 s-1, an energy resolution corresponding to a full width at half maximum of 160 keV over the entire energy range of measured alpha (namely 4 ÷ 6.5 MeV), and the sensitive element’s intrinsic efficiency of about 100%. Being the detector capable of distinguishing alpha energy associated to decays of radon daughters, its applications include 222Rn progeny monitoring. The air sampling system has been realized by a volumetric micro-pump forcing the air-flow through a millipore filter. By knowing the air-flow rate processed and the corresponding alpha energy spectrum measured, the concentrations of 218Po, 214Po and 210Po are determined. The potential alpha energy concentration-in-air is inferred, and effective dose evaluated. Calibration and testing measurements have been carried out by comparing the obtained results to the outputs of professional and expensive radon progeny monitor. The detector capability of “following” radon progeny concentration-in-air vs. time has been demonstrated. The device studied here can be configured as a prototype for an inexpensive radon progeny sensor to be potentially suitable for indoor air-monitoring in residential buildings, evaluating people’s exposures to radon and initiating corrective actions (e.g., mechanical ventilation) if necessary

New developments in the analysis of column-collapse pyroclastic density currents through numerical simulations of multiphase flows

Abstract. A granular multiphase model has been used to evaluate the action of differently sized particles on the dynamics of fountains and associated pyroclastic density currents. The model takes into account the overall disequilibrium conditions between a gas phase and several solid phases, each characterized by its own physical properties. The dynamics of the granular flows (fountains and pyroclastic density currents) has been simulated by adopting a Reynolds-averaged Navier-Stokes model for describing the turbulence effects. Numerical simulations have been carried out by using different values for the eruptive column temperature at the vent, solid particle frictional concentration, turbulent kinetic energy, and dissipation. The results obtained provide evidence of the multiphase nature of the model and describe several disequilibrium effects. The low concentration (≤5 × 10−4) zones lie in the upper part of the granular flow, above the fountain, and above the tail and body of pyroclastic density current as thermal plumes. The high concentration zones, on the contrary, lie in the fountain and at the base of the current. Hence, pyroclastic density currents are assimilated to granular flows constituted by a low concentration suspension flowing above a high concentration basal layer (boundary layer), from the proximal regions to the distal ones. Interactions among the solid particles in the boundary layer of the granular flow are controlled by collisions between particles, whereas the dispersal of particles in the suspension is determined by the dragging of the gas phase. The simulations describe well the dynamics of a tractive boundary layer leading to the formation of stratified facies during Strombolian to Plinian eruptions

Indoor radon survey in university buildings: a case study of Sapienza - University of Rome

The indoor radon concentration in underground workplaces pertaining to Sapienza – University of Rome have been monitored since the 90’s according to prescription of Italian Legislative Decree 230/95. In the last years, the recommendations contained in the Council Directive 2013/59/Euratom have shifted the focus to all indoor exposure situations by promoting actions to identify workplaces and dwellings with radon concentrations exceeding the reference level of 300 Bq/m3. In response to the upcoming transposition into national legislation, Sapienza has promoted the first Italian survey addressing workplaces in university buildings, regardless of the position with respect to the ground floor. The survey has interested more than three hundred workplaces, i.e. administration and professors’ offices, research and educational laboratories, conference rooms and classrooms, distributed in fifteen different buildings. Places monitored are strongly heterogeneous in terms of users’ habit, occupancy pattern and building characteristics. The influence of these parameters into seasonal variation have been addressed by organizing the survey in four quarters. The indoor radon concentration is measured by solid state nuclear track detectors, CR39. The aim of the paper is to present features, methods and intermediate results of the survey. The work, relying on the analysis of previous measurements interesting underground workplaces, focuses on methodology followed during all the preliminary and preparatory phases: active measurements by ionization chamber radon continuous monitor, radon progeny equilibrium factor estimations by radon daughters monitor, strategies for occupants’ awareness, positioning protocol and provisions to maximize representativity of results

Radon concentration in self-bottled mineral spring waters as a possible public health issue

Since 2013, the Council Directive 2013/51/Euratom has been regulating the content of radioactive substances in water intended for human consumption. However, mineral waters are exempted from this regulation, including self-bottled springs waters, where higher radon concentration are expected. Therefore, a systematic survey has been conducted on all the 33 mineral spring waters of Lazio (a region of Central Italy) in order to assess if such waters, when self-bottled, may be of concern for public health. Waters have been sampled in two different ways to evaluate the impact of bottling on radon concentration. Water sampling was possible for 20 different spring waters, with 6 samples for each one. The results show that 2 (10%) of measured mineral spring waters returned radon concentrations higher than 100 Bq L−1, i.e., the parametric value established by the Council Directive. These results, if confirmed by other surveys involving a higher number of mineral spring waters, would suggest regulating also these waters, especially in countries like Italy for which: (i) mineral water consumption is significant; (ii) mineral concession owners generally allow the consumers to fill bottles and containers, intended for transport and subsequent consumption, directly from public fountains or from fountains within the plant; (iii) the consumers’ habit of drinking self-bottled mineral water is widespread

Local BDNF Delivery to the Injured Cervical Spinal Cord using an Engineered Hydrogel Enhances Diaphragmatic Respiratory Function.

We developed an innovative biomaterial-based approach to repair the critical neural circuitry that controls diaphragm activation by locally delivering brain-derived neurotrophic factor (BDNF) to injured cervical spinal cord. BDNF can be used to restore respiratory function via a number of potential repair mechanisms; however, widespread BDNF biodistribution resulting from delivery methods such as systemic injection or lumbar puncture can lead to inefficient drug delivery and adverse side effects. As a viable alternative, we developed a novel hydrogel-based system loaded with polysaccharide-BDNF particles self-assembled by electrostatic interactions that can be safely implanted in the intrathecal space for achieving local BDNF delivery with controlled dosing and duration. Implantation of BDNF hydrogel after C4/C5 contusion-type spinal cord injury (SCI) in female rats robustly preserved diaphragm function, as assessed b

A coupled stability and eco-hydrological model to predict shallow landslides

Knowledge of spatio-temporal dynamics of soil water content, groundwater and infiltration processes is of considerable importance for the understanding and prediction of landslides. Rainfall and consequent water infiltration affect slope stability in various ways, mainly acting on the pore pressure distribution whose increase causes a decrease of the shearing resistance of the soil. For such reasons rainfall and transient changes in the hydrological systems are considered the most common triggers of landslides. So far, the difficulty to monitor groundwater levels or soil moisture contents in unstable terrain have made modeling of landslide a complex issue. At the present, the availability of sophisticated hydrological and physically based models, able to simulate the main hydrological processes, has allowed the development of coupled hydrologicalstability models able to predict when and where a failure could occur. In this study, a slope-failure module, with capability to predict shallow landslides, implemented into an ecohydrological model, tRIBS-VEGGIE (Triangulated Irregular Network (TIN)-based Real-time Integrated Basin Simulator with VEGetation Generator for Interactive Evolution), is presented. The model evaluates the stability dynamics in term of factor of safety consequent to the soil moisture dynamics, strictly depending on the textural soil characteristics and hillslope geometry. Failure criterion used to derive factor of safety equation accounts for the stabilizing effect of matric suction arising in unsaturated soils. The eco-hydrological framework allows also to take into account the effect of vegetation with its cohesive effect as well as its weight load. The Mameyes basin, located in the Luquillo Experimental Forest in Puerto Rico, has been selected for modeling based on the availability of soil, vegetation, topographical, meteorological and historic landslide data. A static analysis based on susceptibility mapping approach was also carried out on the same area at a larger spatial scale, providing the hot spot of landsliding area. Application of the model yields a temporal and spatial distribution of predicted rainfall-induced landslides. Moreover, stability dynamics have been assessed for different meteorological forcing and soil types, to better evaluate the influence of hydrological dynamics on slope stability

Early phrenic motor neuron loss and transient respiratory abnormalities following unilateral cervical spinal cord contusion

Contusion-type cervical spinal cord injury (SCI) is one of the most common forms of SCI observed in patients. In particular, injuries targeting the C3-C5 region affect the pool of phrenic motor neurons (PhMNs) that innervates the diaphragm, resulting in significant and often chronic respiratory dysfunction. Using a previously described rat model of unilateral midcervical C4 contusion with the Infinite Horizon Impactor, we have characterized the early time course of PhMN degeneration and consequent respiratory deficits following injury, as this knowledge is important for designing relevant treatment strategies targeting protection and plasticity of PhMN circuitry. PhMN loss (48% of the ipsilateral pool) occurred almost entirely during the first 24 h post-injury, resulting in persistent phrenic nerve axonal degeneration and denervation at the diaphragm neuromuscular junction (NMJ). Reduced diaphragm compound muscle action potential amplitudes following phrenic nerve stimulation were observed as early as the first day post-injury (30% of pre-injury maximum amplitude), with slow functional improvement over time that was associated with partial reinnervation at the diaphragm NMJ. Consistent with ipsilateral diaphragmatic compromise, the injury resulted in rapid, yet only transient, changes in overall ventilatory parameters measured via whole-body plethysmography, including increased respiratory rate, decreased tidal volume, and decreased peak inspiratory flow. Despite significant ipsilateral PhMN loss, the respiratory system has the capacity to quickly compensate for partially impaired hemidiaphragm function, suggesting that C4 hemicontusion in rats is a model of SCI that manifests subacute respiratory abnormalities. Collectively, these findings demonstrate significant and persistent diaphragm compromise in a clinically relevant model of midcervical contusion SCI; however, the therapeutic window for PhMN protection is restricted to early time points post-injury. On the contrary, preventing loss of innervation by PhMNs and/or inducing plasticity in spared PhMN axons at the diaphragm NMJ are relevant long-term targets