Extracellular RNAs as potential biomarkers for placental dysfunction

Placental dysfunction affects approximately 1 in 10 pregnant women in both the developed and developing worlds. Most commonly, it is manifested as preeclampsia or fetal growth restriction. Over the past two decades, an increasing body of research into the developmental biology of the placenta has been amassed, which points to defects in the differentiation of the trophoblast cell lineage as a key player in the pathophysiology of placental dysfunction. A number of clinical parameters are known to be associated with an elevated risk of placental dysfunction. These include maternal risk factors (such as chronic hypertension, renal disease, and lupus), history of placental dysfunction in a prior pregnancy, abnormalities in the levels of certain proteins in the maternal blood that are commonly used to estimate the risk of fetal genetic defects, and abnormalities in uterine artery Doppler waveforms. These current methods have significant drawbacks, including low specificity and sensitivity, high cost, lack of widespread availability, and lack of validity early in pregnancy. In order to provide a more cost-effective and reliable method to detect an elevated risk for placental dysfunction early in pregnancy, we explored the potential for extracellular RNAs (exRNA) in the maternal serum to be used as biomarkers. In our study, we used next generation sequencing technologies to compare extracellular microRNA (miRNA) levels in serum samples of pregnant women of different gestational ages, nonpregnant women, and placental tissue samples. We discovered that the large majority of microRNAs that were present at higher levels in pregnant serum samples than nonpregnant serum samples and were likely of placental origin. We also found that these pregnancy-specific miRNAs were enriched for miRNAs encoded on chromosomes (Chr) 14 and 19, with changes in the relative expression of these two groups of miRNAs throughout pregnancy. Moreover, the miRNA signatures of late gestational pregnant samples correlated more closely with placental tissue samples than those of early pregnant samples, which could be related to the increasing impact of a larger placenta on the maternal serum exRNA profile. Our results demonstrate the potential utility of next generation sequencing technologies in regards to differentiating between different conditions using clinical samples

Modelling the overheating risk in an uniform high-rise building design with a consideration of urban context and heatwaves

Overheating in buildings is one of the increasing concerns related to climate change and can lead to an increase in heat-related health issues and higher energy consumption due to the use of air conditioning systems. Literature shows that internal conditions and demand on environmental control systems can vary with height within buildings. However, an architectural trend towards highly glazed façades for tall buildings suggests the vertical gradient of performance is not always considered in the design process. By simulating a high-rise residential building in London, a comparative analysis of the overheating risks and daylighting at different levels in the building was conducted. In this study the model was able to consider the influence of surrounding built environment on solar gain and so influence of urban location on overheating risk was taken into account. Simulations were conducted using typical reference years as well as meteorological data for specific heat-wave periods experienced in London and that are expected to become more intense and frequent due to climate change. Passive mitigation options (external shading) are demonstrated to help reduce overheating occurrence by 74%, at the same time the impact of decreased daylighting (30%) is less problematic at higher levels where daylight factor is greater

Purification of olive mill waste: a circular economy model for the Mediterranean region

When olive mill wastewater is given directly to nature without being treated, natural waters become coloured, aquatic life is adversely affected, surface and underground waters are polluted, bad odours occur, and soil quality deteriorates. For these reasons, waste water is not allowed to be poured into soil and water without being discharged. This problem has become a problem that needs to be solved for the Mediterranean countries. With this study, acid cracking and chemical treatability studies achieved the conversion of the physicochemical pretreatment process of olive black water to an automation system. With the applied processes, removal efficiencies of 85% for Chemical Oxygen Demand, 99% for suspended solids, 97% for oil grease and 92% for phenol were obtained. Since the mixing processes of the tanks in the chemical additions are long, the heating chamber in the acid cracking process is designed larger than the tanks. Thus, when there is a black water density in the system, the heating tank in this system can be used as a heating and resting tank in sudden densities in this system. This waste separation process includes producing raw materials, protecting natural resources, and increasing sustainability. In particular, separating the phenol material and making it usable have been essential gains

Changes in insulin sensitivity and lipid profile markers following initial and secondary bouts of multiple eccentric exercises

An acute bout of eccentric exercise affects insulin sensitivity and lipid profile, but how the magnitude of muscle damage affects them is not clear. We compared changes in blood insulin sensitivity and lipid markers after the first (EC1) and second (EC2) eccentric exercise bouts. Fifteen sedentary young men performed arm, leg and trunk muscle eccentric exercises, and repeated them 2 weeks later. Fasting blood samples were taken before, 2 h and 1–5 days after each exercise bout to analyze plasma creatine kinase (CK) activity, serum glucose (GLU), insulin, homeostasis model assessment (HOMA), triacylglycerols (TG), total (TC) and low- (LDLC) and high-density lipoprotein cholesterol (HDLC) concentrations as well as TC/HDLC ratio. Changes in these measures were compared between bouts and relationships to peak plasma CK activity were analyzed. Plasma CK activity increased (p \u3c 0.05) after EC1 (peak: 101,668 ± 58,955 IU/L) but not after EC2. The magnitude of changes in GLU (peak after EC1: 26 ± 10% vs. EC2: 7 ± 6%), insulin (46 ± 27% vs. 15 ± 8%), HOMA (86 ± 48% vs. 24 ± 15%), TC (−20 ± 5% vs. −6 ± 4%), TG (−32 ± 11% vs. −6 ± 3%), LDHC (−47 ± 15% vs. −12 ± 9%), HDLC (35 ± 26% vs. 7 ± 4%), and TC/HDLC ratio (−139 ± 13% vs. −11 ± 7%) were significantly greater after EC1 than EC2. Peak plasma CK activity was significantly (p \u3c 0.05) correlated with the peak changes in blood insulin sensitivity and lipid markers for the combined data of EC1 and EC2. These results suggest that the greater the magnitude of muscle damage, the greater the magnitude of changes in the insulin sensitivity to a negative direction and lipid markers to a positive direction

LEGaTO: first steps towards energy-efficient toolset for heterogeneous computing

LEGaTO is a three-year EU H2020 project which started in December 2017. The LEGaTO project will leverage task-based programming models to provide a software ecosystem for Made-in-Europe heterogeneous hardware composed of CPUs, GPUs, FPGAs and dataflow engines. The aim is to attain one order of magnitude energy savings from the edge to the converged cloud/HPC.Peer ReviewedPostprint (author's final draft

Teratoma Generation in the Testis Capsule

Pluripotent stem cells (PSCs) have the unique characteristic that they can differentiate into cells from all three germ layers. This makes them a potentially valuable tool for the treatment of many different diseases. With the advent of induced pluripotent stem cells (iPSCs) and continuing research with human embryonic stem cells (hESCs) there is a need for assays that can demonstrate that a particular cell line is pluripotent. Germline transmission has been the gold standard for demonstrating the pluripotence of mouse embryonic stem cell (mESC) lines1,2,3. Using this assay, researchers can show that a mESC line can make all cell types in the embryo including germ cells4. With the generation of human ESC lines5,6, the appropriate assay to prove pluripotence of these cells was unclear since human ESCs cannot be tested for germline transmission. As a surrogate, the teratoma assay is currently used to demonstrate the pluripotency of human pluripotent stem cells (hPSCs)7,8,9. Though this assay has recently come under scrutiny and new technologies are being actively explored, the teratoma assay is the current gold standard7. In this assay, the cells in question are injected into an immune compromised mouse. If the cells are pluripotent, a teratoma will eventually develop and sections of the tumor will show tissues from all 3 germ layers10. In the teratoma assay, hPSCs can be injected into different areas of the mouse. The most common injection sites include the testis capsule, the kidney capsule, the liver; or into the leg either subcutaneously or intramuscularly11. Here we describe a robust protocol for the generation of teratomas from hPSCs using the testis capsule as the site for tumor growth

Nigeria's transition to a circular economy: challenges, opportunities and future perspectives

The concept of a circular economy (CE) is a sustainable development strategy aimed at minimising waste and maximising resource efficiency. This paper explores the transition towards a circular economy in Nigeria, providing an overview of the challenges, opportunities, and future perspectives. Nigeria is rich in natural resources and has significant economic potential. However, the country faces severe environmental, economic, and social challenges that could be met with a circular economy. Although Nigeria has abundant natural resources and great economic potential, it faces many significant environmental, economic, and social challenges that must be addressed to move to a circular economy as a viable solution. The methods used in research are based on a mixed-methods research approach; the study synthesises findings from a comprehensive literature review, case studies of Nigerian businesses adopting CE practices, and insights from a SWOT analysis conducted in consultation with experts. The conclusions of the SWOT analysis, enriched by discussions with experts, form the basis for a set of targeted recommendations. These recommendations are aimed at policymakers, business leaders, and practitioners, outlining strategic actions to overcome the identified barriers and harness the opportunities inherent in a circular economy. This paper contributes to the scholarly discourse on sustainability and serves as a practical guide for other developing countries navigating the transition to a circular economy. It underscores the urgency and feasibility of adopting circular economy principles in Nigeria, highlighting the path towards a more sustainable, efficient, and economically robust future. In the study, prospects for Nigeria to benefit from include increased resource efficiency, job creation, economic diversification, and environmental sustainability if the circular economy approach were applied

Análisis estructural y modal de las placas adaptación para martillos hidráulicos y minicargadores en condiciones reales de operación

En Perú, la necesidad de proyectos de servicios como la instalación de redes de gas para los residentes, ha incrementado la demanda del uso de martillos hidráulicos (HH) con cargadores compactos debido a la baja inversión en comparación con otros equipos de maquinaria y la versatilidad. La facilidad para intercambiar martillos hidráulicos con cucharones para completar las etapas de demolición y limpieza ofrecen para los contratistas una alta productividad en comparación con una operación manual. Por esa razón, el software de diseño virtual Inventor Professional fue usado para diseñar una placa de adaptación con la resistencia y durabilidad adecuadas, lo cual tiene un impacto directo sobre la estructura del martillo hidráulico y el brazo hidráulico del cargador compacto. Simultáneamente, se desarrolló una animación básica para explicar el efecto del estilo de operación sobre los martillos hidráulicos y las placas de adaptación. Finalmente, para este desarrollo, se consideró la construcción de la placa de adaptación como un fusible en el sistema, en caso de que los operadores excedan la capacidad de resistencia de la estructura del martillo hidráulico.//In Perú the need for utility projects such as gas network installation for residents has increased the demand for the use of Hydraulic Hammers (HH) with mini-loaders, due to the low investment required compared to other machinery equipment and to the versatility. The easiness to interchange hydraulic hammers with buckets to complete the demolition and cleaning stages offers for contractors a higher productivity than manual operations. For that reason, the virtual design software Inventor Professional was used to design a suitable adapter plate with adequate resistance and durability, which has a direct impact on the structure of the hydraulic hammer and the hydraulic arm of the skid steers. Simultaneously, a basic animation was developed to explain the effect of the operation style over the hydraulic hammers and the adapter plates. Finally, for this development, it was considered the construction of an adapter plate as a fuse in the system, in case operators exceed the resistance capacity of the hydraulic hammer structure