Illumination uniformity in endoscopic imaging

Standardised endoscopic digital images were taken and analysed using an image analysis software (National Instruments Vision Assistant version 7.1.1). The luminance plane was extracted and the pixel intensity distribution was determined along a horizontal line at the position of highest average intensity (centroid). The data was exported to MS Excel and the pixel intensity (y-axis) was plotted against pixel position (x-axis). A trendline using a 2nd order polynomial curve was fitted to each data set. The resultant equation for each curve was compared with equations obtained from other images taken under various illumination conditions and settings

Controlled growth factor release from synthetic extracellular matrices

Polymeric matrices can be used to grow new tissues and organs(1,2), and the delivery of growth factors from these matrices is one method to regenerate tissues(3,4). A problem with engineering tissues that exist in a mechanically dynamic environment, such as bone, muscle and blood vessels(5,6), is that most drug delivery systems have been designed to operate under static conditions. We thought that polymeric matrices, which release growth factors in response to mechanical signals, might provide a new approach to guide tissue formation in mechanically stressed environments. Critical design features for this type of system include the ability to undergo repeated deformation, and a reversible binding of the protein growth factors to polymeric matrices to allow for responses to repeated stimuli. Here we report a model delivery system that can respond to mechanical signalling and upregulate the release of a growth factor to promote blood vessel formation. This approach may rnd a number of applications, including regeneration and engineering of new tissues and more general drug-delivery applications.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62980/1/408998a0.pd

Modeling UV and X-Ray Emission in a Post-CME Current Sheet

A post-CME current sheet (CS) is a common feature developed behind an erupting flux rope in CME models. Observationally, white light observations have recorded many occurrences of a thin ray appearing behind a CME eruption that closely resembles a post-CME CS in its spatial correspondence and morphology. UV and X-ray observations further strengthen this interpretation by the observations of high temperature emission at locations consistent with model predictions. The next question then becomes whether the properties inside a post-CME CS predicted by a model agree with observed properties. In this work, we assume that the post-CME CS is a consequence of Petschek-like reconnection and that the observed ray-like structure is bounded by a pair of slow mode shocks developed from the reconnection site. We perform time-dependent ionization calculations and model the UV line emission. We find that such a model is consistent with SOHO/UVCS observations of the post-CME CS. The change of Fe XVIII emission in one event implies an inflow speed of ~10 km/s and a corresponding reconnection rate of M_A ~ 0.01. We calculate the expected X-ray emission for comparison with X-ray observations by Hinode/XRT, as well as the ionic charge states as would be measured in-situ at 1 AU. We find that the predicted count rate for Hinode/XRT agree with what was observed in a post-CME CS on April 9, 2008, and the predicted ionic charge states are consistent with high ionization states commonly measured in the interplanetary CMEs. The model results depend strongly on the physical parameters in the ambient corona, namely the coronal magnetic field, the electron density and temperature during the CME event. It is crucial to obtain these ambient coronal parameters and as many facets of the CS properties as possible by observational means so that the post-CME current sheet models can be scrutinized more effectively

Using music and motion analysis to construct 3D animations and visualisations

This paper presents a study into music analysis, motion analysis and the integration of music and motion to form creative natural human motion in a virtual environment. Motion capture data is extracted to generate a motion library, this places the digital motion model at a fixed posture. The first step in this process is to configure the motion path curve for the database and calculate the possibility that two motions were sequential through the use of a computational algorithm. Every motion is then analysed for the next possible smooth movement to connect to, and at the same time, an interpolation method is used to create the transitions between motions to enable the digital motion models to move fluently. Lastly, a searching algorithm sifts for possible successive motions from the motion path curve according to the music tempo. It was concluded that the higher ratio of rescaling a transition, the lower the degree of natural motio

PmoB subunit of particulate methane monooxygenase (pMMO) in Methylococcus capsulatus (Bath): The Cu^I sponge and its function

In this study, we describe efforts to clarify the role of the copper cofactors associated with subunit B (PmoB) of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) (M. capsulatus). This subunit exhibits strong affinity toward Cu^I ions. To elucidate the high copper affinity of the subunit, the full-length PmoB, and the N-terminal truncated mutants PmoB_(33–414) and PmoB_(55–414), each fused to the maltose-binding protein (MBP), are cloned and over-expressed into Escherichia coli (E. coli) K12 TB1 cells. The Y374F, Y374S and M300L mutants of these protein constructs are also studied. When this E. coli is grown with the pmoB gene in 1.0 mM Cu^(II), it behaves like M. capsulatus (Bath) cultured under high copper stresswith abundant membrane accumulation and high CuI content. The recombinantPmoB proteins are verified by Western blotting of antibodies directed against the MBP sub-domain in each of the copper-enriched PmoB proteins. Cu K-edge X-rayabsorption near edge spectroscopy (XANES) of the copper ions confirms that all the PmoB recombinants are Cu^I proteins. All the PmoB proteins show evidence of a “dicopper site” according to analysis of the Cu extended X-ray absorption edge fine structure (EXAFS) of the membranes. No specific activities toward methane and propene oxidation are observed with the recombinant membrane-bound PmoB proteins. However, significant production of hydrogen peroxide is observed in the case of the PmoB_(33–414) mutant. Reaction of the dicopper site with dioxygenproduces hydrogen peroxide and leads to oxidation of the CuI ions residing in the C-terminal sub-domain of the PmoB subunit

Tellurium substitution effect on superconductivity of the alpha-phase Iron Selenide

We have carried out a systematic study of the PbO-type compound FeSe_{1-x}Te_x (x = 0~1), where Te substitution effect on superconductivity is investigated. It is found that superconducting transition temperature reaches a maximum of Tc=15.2K at about 50% Te substitution. The pressure-enhanced Tc of FeSe0.5Te0.5 is more than 10 times larger than that of FeSe. Interestingly, FeTe is no longer superconducting. A low temperature structural distortion changes FeTe from triclinic symmetry to orthorhombic symmetry. We believe that this structural change breaks the magnetic symmetry and suppresses superconductivity in FeTe.Comment: Some typing errors are corrected; we take out one figures, now the paper has 14 pages, 5 figure

Intra-arterial delivery of triolein emulsion increases vascular permeability in skeletal muscles of rabbits

<p>Abstract</p> <p>Background</p> <p>To test the hypothesis that triolein emulsion will increase vascular permeability of skeletal muscle.</p> <p>Methods</p> <p>Triolein emulsion was infused into the superficial femoral artery in rabbits (triolein group, n = 12). As a control, saline was infused (saline group, n = 18). Pre- and post-contrast T1-weighted MR images were obtained two hours after infusion. The MR images were qualitatively and quantitatively evaluated by assessing the contrast enhancement of the ipsilateral muscles. Histologic examination was performed in all rabbits.</p> <p>Results</p> <p>The ipsilateral muscles of the rabbits in the triolein group showed contrast enhancement, as opposed to in the ipsilateral muscles of the rabbits in the saline group. The contrast enhancement of the lesions was statistically significant (p < 0.001). Histologic findings showed that most examination areas of the triolein and saline groups had a normal appearance.</p> <p>Conclusion</p> <p>Rabbit thigh muscle revealed significantly increased vascular permeability with triolein emulsion; this was clearly demonstrated on the postcontrast MR images.</p

A Very High Energy Gamma-Ray Spectrum of 1ES 2344+514

The BL Lacertae (BL Lac) object 1ES 2344+514 (1ES 2344), at a redshift of 0.044, was discovered as a source of very high energy (VHE) gamma rays by the Whipple Collaboration in 1995 \citep{2344Catanese98}. This detection was recently confirmed by the HEGRA Collaboration \citep{2344Hegra03}. As is typical for high-frequency peaked blazars, the VHE gamma-ray emission is highly variable. On the night of 20 December, 1995, a gamma-ray flare of 5.3-sigma significance was detected, the brightest outburst from this object to-date. The emission region is compatible with a point source. The spectrum between 0.8 TeV and 12.6 TeV can be described by a power law $\frac{ ^3 N}{ E A t}=(5.1\pm1.0_{st}\pm1.2_{sy})\times10^{-7} (E/ \mathrm{TeV})^{-2.54 \pm0.17_{st}\pm0.07_{sy}} \mathrm{\frac{1}{TeV m^2 s}}$. Comparing the spectral index with that of the other five confirmed TeV blazars, the spectrum of 1ES 2344 is similar to 1ES 1959+650, located at almost the same distance. The spectrum of 1ES 2344 is steeper than the brightest flare spectra of Markarian 421 (Mrk~421) and Markarian 501 (Mrk~501), both located at a distance about 2/3 that of 1ES 2344, and harder than the spectra of PKS 2155-304 and H~1426+428, which are located almost three times as far. This trend is consistent with attenuation caused by the infrared extragalactic background radiation.Comment: 27 pages, 11 figures, accepted by Ap

Second-hand smoke and chronic bronchitis in Taiwanese women: a health-care based study

<p>Abstract</p> <p>Background</p> <p>Cigarette smoking cannot fully explain the epidemiologic characteristics of chronic obstructive pulmonary disease (COPD) in women, particularly for those who rarely smoke, but COPD risk is not less than men. The aim of our study is to investigate the relationship between second-hand smoke (SHS) exposure and chronic bronchitis in Taiwanese women.</p> <p>Methods</p> <p>We used Taiwan's National Health Insurance Bureau claims data in 1999, and cross-checked using criteria set by the American Thoracic Society; there were 33 women with chronic bronchitis, 182 with probable chronic bronchitis, and 205 with no chronic bronchitis during our interview time between 2000 and 2005. We measured second-hand smoke (SHS) exposure by self-reported measures (household users and duration of exposure), and validated this by measuring urinary cotinine levels of a subset subjects. Classification of chronic bronchitis was also based on spirometry defined according to the GOLD guidelines to get the severity of COPD.</p> <p>Results</p> <p>Women who smoked and women who had been exposed to a lifetime of SHS were 24.81-fold (95% CI: 5.78-106.38) and 3.65-fold (95% CI: 1.19-11.26) more likely to have chronic bronchitis, respectively, than those who had not been exposed to SHS. In addition, there was a significant increasing trend between the severity of COPD and exposure years of SHS (<it>p </it>< 0.01). The population attributable risk percentages of chronic bronchitis for smokers and those exposed to SHS were 23.2 and 47.3% respectively.</p> <p>Conclusions</p> <p>These findings indicate that, besides cigarette smoking, exposure to SHS is a major risk factor for chronic bronchitis in Taiwanese women.</p