33 research outputs found
Does inbreeding affects developmental stability in Drosophila subobscura populations?
In the present paper, we focused on the coadaptive aspect of genetic variability at population level and its relation to genomic stress such as inbreeding. The paper evaluates the effects of an experimental reduction of average heterozygosity after fourteen generations of systematic inbreeding in laboratory conditions, on developmental stability in Drosophila subobscura populations from two ecologically and topologically distinct habitats, knowing that they possess a certain degree of genetic differences due to their different evolutionary histories. The aims were to analyze: (i) the variability change of wing size (length and width) among the inbred lines from both populations; (ii) the relations between homozigosity and level of fluctuating asymmetry as a potential measure of developmental instability, in inbred lines originating from two populations. Results for the wing size showed similar between line variability pattern across generations of systematic inbreeding in both populations. The obtained results suggest that variability of fluctuating asymmetry as a measure of developmental instability can not be related to homozygosity due to inbreeding per se, in both experimental populations.Rad je fokusiran na ko adaptivni aspekt genetiÄke varijabilnosti na nivou populacije i u odnosu na genomski stres kao Å”to je inbriding. Analizirani su efekti eksperimentalnog smanjenja proseÄne heterozigotnosti nakon 14 generacija sistematskog inbridinga u laboratorijskim uslovima na razvojnu stabilnost Drosophila subobscura populacija sa dva ekoloÅ”ki i topoloÅ”ki odvojena staniÅ”ta, znajuÄi da one poseduju odredjeni stepen genetiÄke diferencijacije usled razliÄitih evolutivnih istorija. Ciljevi rada su bili da se analizira: (i) varijabilnost u promeni veliÄine krila (dužine i Å”irine) medju inbidingovanim linijama i populacijama; (ii) odnosi izmedju homozigotizacije i nivoua fluktuirajuÄe asimetrije kao potencijalne mere razvojne nestabilnosti u inbridingovanim linijama obe populacije. Rezultati veliÄine krila pokazuju sliÄnu varijabilnost medju linijama obe populacije kroz generacije inbridinga. Dobijeni rezultati sugeriÅ”u da varijabilnost fluktuirajuÄe asimetrije kao mere razvojne nestabilnosti ne mogu biti povezani sa homozigotizacijom usled inbridinga per se, u obe eksperimentalne populacije.Projekat ministarstva br. 17301
Intra-species differentiation among Drosophila subobscura from different habitats in Serbia
Adaptation to different environmental conditions is a natural phenomenon that potentially leads to population subdivision. We surveyed genetic differentiation in inversion polymorphism within populations of Drosophila subobscura sampled in three ecologically different forest communities. The analysis of inversion polymorphism revealed significant differences between some pairs of samples in some gene arrangement frequencies of the A, U, and E chromosomes and some karyotype combination frequencies of the U chromosome, but significant differentiation within populations was not observed. It cannot be decided which evolutionary forces are responsible for the observed variability in inversion polymorphism.Adaptacija na razliÄite sredinske uslove može uticati na diferencijaciju u okviru populacija. U ovom radu analizirana je genetiÄka diferencijacija po inverzionom polimorfizmu u okviru populacija D. subobscura sakupljenih u tri ekoloÅ”ki razliÄita staniÅ”ta. UtvrÄene su znaÄajne razlike u uÄestalosti pojedinih hromozomskih aranžmana A, U i E hromozoma, i uÄestalosti pojedinih kombinacija kariotipova U hromozoma, ali nije utvrÄena diferencijacija u okviru populacija. Razmatrani su evolutivni faktori koji bi mogli da budu odgovorni za uoÄenu varijabilnost u inverzionom polimorfizmu.Projekat ministarstva br. 14301
Sexual selection can reduce mutational load in Drosophila subobscura
According to theoretical predictions sexual selection can reduce mutational load through male mating success. Males of good genetic quality should be more successful in matings, compared to the males of low genetic quality, thus in this way females can prevent deleterious alleles to be transmitted to the next generation. We tested this hypothesis through set up of two experimental groups from same genetic pool, where in one group genetic quality was manipulated by ionizing radiation. Within each group opportunity for choosing mates was imposed: males and females had no choice or had multiple choice. Mutational load was measured through the variability of different fitness components: fecundity and egg-to-adult viability. Our results indicate that sexual selection can reduce mutational load, only for fecundity. Group with the presence of female choice exhibited higher fecundity than group in which sexual selection was experimentally eliminated, but only in 'irradiated' group. There was no overall difference in egg-to-adult viability between different sexual selection regimes in any of the group. It should be considered that sexual selection can cause sexual conflict, and potential opposite effects of sexual selection and sexual conflict on fitness. Genetic structure of populations, in terms of the level of mutational load, is an important factor which can determinate the role of sexual selection.Prema teorijskim oÄekivanjima seksualna selekcija može smanjivati mutaciona optereÄenja u populacijama, preko uspeÅ”nosti mužjaka u parenju. Mužjaci koji su boljeg genetiÄkog kvaliteta bi trebalo da budu uspeÅ”niji u parenju od mužjaka loÅ”ijeg genetiÄkog kvaliteta. Na taj naÄin bi ženke mogle da smanje prenoÅ”enje Å”tetnih alela u sledeÄu generaciju. Ova hipoteza je testirana uspostavljanjem dve eksperimentalne grupe od istog genetiÄkog pula jedinki, pri Äemu su u jednoj mutacije indukovane jonizujuÄim zraÄenjem. Unutar svake grupe je nametnuta moguÄnost izbora u parenju: mužjaci i ženke nisu imali izbor, ili su imali viÅ”estruki izbor. Mutaciona optereÄenja su merena preko dve komponente adaptivne vrednosti: fekunditeta i preživljavanja od stadijuma jaja do adulta. Dobijeni rezultati ukazuju da seksualna selekcija može imati uticaj na smanjivanje mutacionih optereÄenja ali samo za fekunditet. U prisustvu seksualne selekcije su vrednosti fekunditeta bile veÄe u odnosu na vrednosti koje su dobijene kad je seksualna selekcija eksperimentalno uklonjena, ali samo u okviru 'ozraÄene' grupe. Razlike u preživljavanju od jaja do adulta izmeÄu razliÄitih režima seksualne selekcije nisu dobijene ni u jednoj od grupa. Kako seksualna selekcija Äesto uzrokuje i seksualni konflikt, treba uzeti u obzir njihovo potencijalno delovanje u suprotnim pravcima na adaptivnu vrednost. GenetiÄka struktura populacija, koja podrazumeva i veliÄinu mutacionih optereÄenja, može biti važan faktor od koga Äe i zavisiti delovanje seksualne selekcija na adaptivnu vrednost.Projekat ministarstva br. 17301
Does inbreeding affects developmental stability in Drosophila subobscura populations?
In the present paper, we focused on the coadaptive aspect of genetic variability at population level and its relation to genomic stress such as inbreeding. The paper evaluates the effects of an experimental reduction of average heterozygosity after fourteen generations of systematic inbreeding in laboratory conditions, on developmental stability in Drosophila subobscura populations from two ecologically and topologically distinct habitats, knowing that they possess a certain degree of genetic differences due to their different evolutionary histories. The aims were to analyze: (i) the variability change of wing size (length and width) among the inbred lines from both populations; (ii) the relations between homozigosity and level of fluctuating asymmetry as a potential measure of developmental instability, in inbred lines originating from two populations. Results for the wing size showed similar between line variability pattern across generations of systematic inbreeding in both populations. The obtained results suggest that variability of fluctuating asymmetry as a measure of developmental instability can not be related to homozygosity due to inbreeding per se, in both experimental populations.Rad je fokusiran na ko adaptivni aspekt genetiÄke varijabilnosti na nivou populacije i u odnosu na genomski stres kao Å”to je inbriding. Analizirani su efekti eksperimentalnog smanjenja proseÄne heterozigotnosti nakon 14 generacija sistematskog inbridinga u laboratorijskim uslovima na razvojnu stabilnost Drosophila subobscura populacija sa dva ekoloÅ”ki i topoloÅ”ki odvojena staniÅ”ta, znajuÄi da one poseduju odredjeni stepen genetiÄke diferencijacije usled razliÄitih evolutivnih istorija. Ciljevi rada su bili da se analizira: (i) varijabilnost u promeni veliÄine krila (dužine i Å”irine) medju inbidingovanim linijama i populacijama; (ii) odnosi izmedju homozigotizacije i nivoua fluktuirajuÄe asimetrije kao potencijalne mere razvojne nestabilnosti u inbridingovanim linijama obe populacije. Rezultati veliÄine krila pokazuju sliÄnu varijabilnost medju linijama obe populacije kroz generacije inbridinga. Dobijeni rezultati sugeriÅ”u da varijabilnost fluktuirajuÄe asimetrije kao mere razvojne nestabilnosti ne mogu biti povezani sa homozigotizacijom usled inbridinga per se, u obe eksperimentalne populacije.Projekat ministarstva br. 17301
Intra-species differentiation among Drosophila subobscura from different habitats in Serbia
Adaptation to different environmental conditions is a natural phenomenon that potentially leads to population subdivision. We surveyed genetic differentiation in inversion polymorphism within populations of Drosophila subobscura sampled in three ecologically different forest communities. The analysis of inversion polymorphism revealed significant differences between some pairs of samples in some gene arrangement frequencies of the A, U, and E chromosomes and some karyotype combination frequencies of the U chromosome, but significant differentiation within populations was not observed. It cannot be decided which evolutionary forces are responsible for the observed variability in inversion polymorphism.Adaptacija na razliÄite sredinske uslove može uticati na diferencijaciju u okviru populacija. U ovom radu analizirana je genetiÄka diferencijacija po inverzionom polimorfizmu u okviru populacija D. subobscura sakupljenih u tri ekoloÅ”ki razliÄita staniÅ”ta. UtvrÄene su znaÄajne razlike u uÄestalosti pojedinih hromozomskih aranžmana A, U i E hromozoma, i uÄestalosti pojedinih kombinacija kariotipova U hromozoma, ali nije utvrÄena diferencijacija u okviru populacija. Razmatrani su evolutivni faktori koji bi mogli da budu odgovorni za uoÄenu varijabilnost u inverzionom polimorfizmu.Projekat ministarstva br. 14301
The effect of lead on the developmental stability of Drosophila subobscura through selection in laboratory conditions
Fluctuating asymmetry (FA), the increased variation of bilateral symmetry in a sample of individuals, can indicate disturbance in developmental stability caused by environmental and/or genomic stress. This developmental instability was analyzed in Drosophila subobscura maintained for seven generations on two different concentrations of lead in laboratory conditions. The FA4 index showed that the genotypes reared on the higher lead concentration were in developmental homeostasis, except for males in the F7 generation, for both wing size parameters. The results show that different degrees of lead pollution cause different responses to selection of the exposed population in laboratory conditions.FluktuirajuÄa asimetrija predstavlja poveÄani stepen variranja bilateralno simetriÄnih karaktera u uzorku individua, i može da bude indikator remeÄenja nivoa razvojne stabilnosti pod uticajem genomskog i/ili sredinskog stresa. Stepen razvojne nestabilnosti je analiziran kod populacije Drosophila subobscura, uzgajane u laboratorijskim uslovima tokom sedam generacija na dve razliÄite koncentracije olova. Rezultati FA4 indeksa su pokazali da genotipovi koji su uzgajani na viÅ”oj koncentraciji olova su u razvojnoj homeostazi, osim mužjaka u F7 generaciji, za oba posmatrana parametra krila. Rezultati ove studije ukazuju na to da razliÄit stepen zagaÄenja olovom u laboratorijskim uslovima može izazivati razliÄite odgovore na selekciju kod tretiranih populacija.Projekat ministarstva br. 14301
Inversion polymorphism in populations of Drosophila subobscura from urban and non-urban environments
Populations of Drosophila subobscura from the urban area of Belgrade and from the locality, Deliblato, which is not under strong anthropogenic influence, were studied with the aim to characterize and compare their genetic structure by examining chromosomal inversion polymorphism. Additional analysis and comparison of this type of polymorphism with several other populations from different habitats in the central Balkans, was done. The obtained results indicate higher heterozygosity in the population from Belgrade. Despite being ecologically marginal and under strong and complex influences, this population did not show a decline in the number of inversions and it is not highly differentiated compared to the referent populations.UraÄena je analiza inverzionog polimorfizma u populacijama Drosophila subobscura poreklom iz urbane sredine, lokalitet BotaniÄka baÅ”ta u Beogradu, i sa lokaliteta u Deliblatu, Srbija, koji nije pod jakim antropogenim uticajem. PoreÄenjem inverzionog polimorfizma ove dve populacije meÄusobno, kao i sa drugim populacijama u regionu, ispitane su karakteristike i dinamika ovog tipa polimorfizma na centralnom Balkanu. Bez obzira Å”to se populacija iz Beograda nalazi pod jakim antropogenim uticajem, ona ne pokazuje smanjenje broja inverzija i nije znaÄajno diferencirana u poreÄenju sa geografski bliskim populacijama. Može se zakljuÄiti da selektivni pritisak uzrokovan zagaÄenjem nije jak ili da je populacija prilagoÄena na zagaÄenje.Projekat ministarstva br. 14301
Sexual selection can reduce mutational load in Drosophila subobscura
According to theoretical predictions sexual selection can reduce mutational load through male mating success. Males of good genetic quality should be more successful in matings, compared to the males of low genetic quality, thus in this way females can prevent deleterious alleles to be transmitted to the next generation. We tested this hypothesis through set up of two experimental groups from same genetic pool, where in one group genetic quality was manipulated by ionizing radiation. Within each group opportunity for choosing mates was imposed: males and females had no choice or had multiple choice. Mutational load was measured through the variability of different fitness components: fecundity and egg-to-adult viability. Our results indicate that sexual selection can reduce mutational load, only for fecundity. Group with the presence of female choice exhibited higher fecundity than group in which sexual selection was experimentally eliminated, but only in 'irradiated' group. There was no overall difference in egg-to-adult viability between different sexual selection regimes in any of the group. It should be considered that sexual selection can cause sexual conflict, and potential opposite effects of sexual selection and sexual conflict on fitness. Genetic structure of populations, in terms of the level of mutational load, is an important factor which can determinate the role of sexual selection.Prema teorijskim oÄekivanjima seksualna selekcija može smanjivati mutaciona optereÄenja u populacijama, preko uspeÅ”nosti mužjaka u parenju. Mužjaci koji su boljeg genetiÄkog kvaliteta bi trebalo da budu uspeÅ”niji u parenju od mužjaka loÅ”ijeg genetiÄkog kvaliteta. Na taj naÄin bi ženke mogle da smanje prenoÅ”enje Å”tetnih alela u sledeÄu generaciju. Ova hipoteza je testirana uspostavljanjem dve eksperimentalne grupe od istog genetiÄkog pula jedinki, pri Äemu su u jednoj mutacije indukovane jonizujuÄim zraÄenjem. Unutar svake grupe je nametnuta moguÄnost izbora u parenju: mužjaci i ženke nisu imali izbor, ili su imali viÅ”estruki izbor. Mutaciona optereÄenja su merena preko dve komponente adaptivne vrednosti: fekunditeta i preživljavanja od stadijuma jaja do adulta. Dobijeni rezultati ukazuju da seksualna selekcija može imati uticaj na smanjivanje mutacionih optereÄenja ali samo za fekunditet. U prisustvu seksualne selekcije su vrednosti fekunditeta bile veÄe u odnosu na vrednosti koje su dobijene kad je seksualna selekcija eksperimentalno uklonjena, ali samo u okviru 'ozraÄene' grupe. Razlike u preživljavanju od jaja do adulta izmeÄu razliÄitih režima seksualne selekcije nisu dobijene ni u jednoj od grupa. Kako seksualna selekcija Äesto uzrokuje i seksualni konflikt, treba uzeti u obzir njihovo potencijalno delovanje u suprotnim pravcima na adaptivnu vrednost. GenetiÄka struktura populacija, koja podrazumeva i veliÄinu mutacionih optereÄenja, može biti važan faktor od koga Äe i zavisiti delovanje seksualne selekcija na adaptivnu vrednost.Projekat ministarstva br. 17301
Synergistic effect of environmental and genomic stress on wing size of drosophila subobscura
Growing anthropogenic influence on every aspect of environment arise important issues regarding the ability of populations and species to adapt to variant pressures. Lead is one of the most present contaminants in the environment with detrimental influence on organisms and populations. In combination with genomic stress, lead may act synergistically, leading to reduction in adaptive values. We sampled two Drosophila subobscura populations, from ecologically different habitats and established differences in genetic backgrounds and population histories. In order to establish different levels of genome heterozygosity, series of intra-line, intra-population and between population crosses were made. The progeny was reared on a standard Drosophila medium and a medium with 200Ī¼g/mL of lead acetate and right wing of approximately 4000 individuals was used for geometric morphometric analysis of wing size. Results showed that lead significantly reduces wing size and that magnitude of this reduction is dependent on genetic background, indicating synergistic effect of genomic and environmental stress. There is also an indication of strong female origin influence on the outcome of hybridization when source of environmental stress is lead. Our results showed that the genetic structure of populations is of great importance for population fitness in anthropogenic induced stressful conditions. Further studies of synergistic effect of genetic and environmental stress are needed, as well as studies of its outcome in natural populations.Genetika (2016), 48(3): 1039-105
Mating behavior as an indicator of quality of Drosophila subobscura males?
According to current theoretical predictions, any deleterious mutations that reduce nonsexual fitness may have a negative influence on mating success. This means that sexual selection may remove deleterious mutations from the populations. Males of good genetic quality should be more successful in mating, compared to the males of lower genetic quality. As mating success is a condition dependent trait, large fractions of the genome may be a target of sexual selection and many behavioral traits are likely to be condition dependent. We manipulated the genetic quality of Drosophila subobscura males by inducing mutations with ionizing radiation and observed the effects of the obtained heterozygous mutations on male mating behavior: courtship occurrence, courtship latency, mating occurrence, latency to mating and duration of mating. We found possible effects of mutations. Females mated more frequently with male progeny of nonirradiated males and that these males courted females faster compared to the male progeny of irradiated males. Our findings indicate a possible important role of sexual selection in purging deleterious mutations