6 research outputs found
The role of myeloperoxidase in the regulation of polymorphonuclear neutrophil cell death
Role of myeloperoxidase in the regulated cell death of polymorphonuclear neutrophils undergoing oxidative burst
Isolation of bovine neutrophils by fluorescence- and magnetic-activated cell sorting
International audienceFlow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production