Characterization of PTZ-Induced Seizure Susceptibility in a Down Syndrome Mouse Model That Overexpresses CSTB

Down syndrome (DS) is a complex genetic syndrome characterized by intellectual disability, dysmorphism and variable additional physiological traits. Current research progress has begun to decipher the neural mechanisms underlying cognitive impairment, leading to new therapeutic perspectives. Pentylenetetrazol (PTZ) has recently been found to have positive effects on learning and memory capacities of a DS mouse model and is foreseen to treat DS patients. But PTZ is also known to be a convulsant drug at higher dose and DS persons are more prone to epileptic seizures than the general population. This raises concerns over what long-term effects of treatment might be in the DS population. The cause of increased propensity for epilepsy in the DS population and which Hsa21 gene(s) are implicated remain unknown. Among Hsa21 candidate genes in epilepsy, CSTB, coding for the cystein protease inhibitor cystatin B, is involved in progressive myoclonus epilepsy and ataxia in both mice and human. Thus we aim to evaluate the effect of an increase in Cstb gene dosage on spontaneous epileptic activity and susceptibility to PTZ-induced seizure. To this end we generated a new mouse model trisomic for Cstb by homologous recombination. We verified that increasing copy number of Cstb from Trisomy (Ts) to Tetrasomy (Tt) was driving overexpression of the gene in the brain, we checked transgenic animals for presence of locomotor activity and electroencephalogram (EEG) abnormalities characteristic of myoclonic epilepsy and we tested if those animals were prone to PTZ-induced seizure. Overall, the results of the analysis shows that an increase in Cstb does not induce any spontaneous epileptic activity and neither increase or decrease the propensity of Ts and Tt mice to myoclonic seizures suggesting that Ctsb dosage should not interfere with PTZ-treatment

COVID-19-Related Thrombotic and Bleeding Events in Adults With Congenital Heart Disease.

BACKGROUND Altered coagulation is a striking feature of COVID-19. Adult patients with congenital heart disease (ACHD) are prone to thromboembolic (TE) and bleeding complications. OBJECTIVES The purpose of this study was to investigate the prevalence and risk factors for COVID-19 TE/bleeding complications in ACHD patients. METHODS COVID-19-positive ACHD patients were included between May 2020 and November 2021. TE events included ischemic cerebrovascular accident, systemic and pulmonary embolism, deep venous thrombosis, myocardial infarction, and intracardiac thrombosis. Major bleeding included cases with hemoglobin drop >2 g/dl, involvement of critical sites, or fatal bleeding. Severe infection was defined as need for intensive care unit, endotracheal intubation, renal replacement therapy, extracorporeal membrane oxygenation, or death. Patients with TE/bleeding were compared to those without events. Factors associated with TE/bleeding were determined using logistic regression. RESULTS Of 1,988 patients (age 32 [IQR: 25-42] years, 47% male, 59 ACHD centers), 30 (1.5%) had significant TE/bleeding: 12 TE events, 12 major bleeds, and 6 with both TE and bleeding. Patients with TE/bleeding had higher in-hospital mortality compared to the remainder cohort (33% vs 1.7%; P < 0.0001) and were in more advanced physiological stage (P = 0.032) and NYHA functional class (P = 0.01), had lower baseline oxygen saturation (P = 0.0001), and more frequently had a history of atrial arrhythmia (P < 0.0001), previous hospitalization for heart failure (P < 0.0007), and were more likely hospitalized for COVID-19 (P < 0.0001). By multivariable logistic regression, prior anticoagulation (OR: 4.92; 95% CI: 2-11.76; P = 0.0003), cardiac injury (OR: 5.34; 95% CI: 1.98-14.76; P = 0.0009), and severe COVID-19 (OR: 17.39; 95% CI: 6.67-45.32; P < 0.0001) were independently associated with increased risk of TE/bleeding complications. CONCLUSIONS ACHD patients with TE/bleeding during COVID-19 infection have a higher in-hospital mortality from the illness. Risk of coagulation disorders is related to severe COVID-19, cardiac injury during infection, and use of anticoagulants

Application of GAPS Software for Kinematic Positioning

W artykule przedstawiono wyniki badań dotyczących wyznaczenia pozycji statku powietrznego w trybie kinematycznym w systemie GPS. W obliczeniach użyto dwuczęstotliwościowych obserwacji kodowo-fazowych GPS z odbiornika Topcon TPS Hiper. Pozycja samolotu została wyznaczona w komercyjnym oprogramowaniu GAPS, przy zastosowaniu metody PPP. Dodatkowo, w artykule scharakteryzowano program GAPS oraz metodę PPP. Wstępne rezultaty z programu GAPS umożliwiają osiągnięcie dokładności około 0,035 m dla współrzędnych horyzontalnych oraz ponad 0,06 m dla współrzędnej wertykalnej. W artykule porównano również wartości błędów średnich z programu GAPS i CSRS-PPP oraz wyznaczono parametr RMS dla wysokości elipsoidalnej H.The paper presents the results on determination of aircraft’s position in kinematic mode in GPS system. The dual-frequency code and phase observations from Topcon TPS Hiper receiver were used for computations. The aircraft’s position in commercial GAPS software was estimated using PPP method. In addition, the GAPS software and PPP method were characterized. Preliminary results from GAPS software show that accuracy for horizontal coordinates is about 0.035 m and for vertical coordinate it is more than 0.06 m, respectively. Mean errors from GAPS and CSRS-PPP software were also compared and RMS parameter for the ellipsoidal height H was determined

Application the GPS code observations in BSSD method for recovery the position of the aircraft

In this paper, the results of aircraft positioning based on GPS code observations in air aviation are presented. The aircraft position was recovery using Between Satellite Single Difference (BSSD) method in GPS system. The BSSD method was applied for designation the precise position of Cessna 172 plane in flight test in air navigation. The coordinates of Cessna 172 plane were determinated using least square estimation in XYZ geocentric frame. The average accuracy of aircraft position equals to 0.797 m for X axis, 0.496 m for Y axis and 0.966 m for Z axis, respectively. In addition, the protection level of HPL parameter amounts to 4.991 m and 5.749 m for VPL term. In paper, the XYZ coordinates of Cessna 172 plane were also compared with PPP solution from GAPS software. The value of RMS bias is about 1.642 m for X axis, 0.902  m for Y axis and 0.892 m for Z axis, respectively

The co-operation of manufacturers of buses and coach hauliers from present the football organizations as way on building picture of these enterprises

Celem niniejszego artykułu jest ukazanie współczesnych organizacji piłkarskich jako przedsiębiorstw skoncentrowanych na maksymalizacji swoich zysków. Ponadto podjęto również próbę określenia roli, jaką spełnia przewóz piłkarzy na mecze w prawidłowym funkcjonowaniu współczesnych, profesjonalnych organizacji piłkarskich. W pierwszej części pracy przedstawiono podobieństwa pomiędzy współczesnymi klubami piłkarskimi a przedsiębiorstwami z innych branż. Następnie omówiono kwestię przewozu autokarowego na mecze ligowe w Lotto Ekstraklasie w sezonie 2016/2017, a także zabezpieczenia transportowego piłkarzy oraz ważnych gości i delegatów podczas piłkarskiego turnieju finałowego Euro 2012. W pracy przedstawiono wybranych producentów autobusowych i przewoźników autokarowych współpracujących z organizacjami piłkarskimi oraz sposoby wykorzystania tego współdziałania przez producentów i przewoźników do budowy wizerunku.present of soccer organizations is the aim of present article. The test of qualification of part moreover was undertaken also, what fulfils the transport of footballers on games in correct functioning the present, professional soccer organizations. It soccer clubs in first part article were introduced was similarities among present, and enterprises from different trades. Talk over on league games the matter of coach transport then in Lotto the Top-league in season 2016/2017, and also the forwarding protection of footballers as well as important it entertains and the delegates during soccer tournament final Euro 2012. In article were introduced the and hauliers coach co-operating from organizations chosen of bus manufacturers soccer the as well as ways utilization of this co-operation by manufacturers and hauliers to building the picture

Preliminary results of DCB C1-C2 in a GPS system

The paper present the results of studies related to estimation of instrumental biases C1-C2 in a GPS system. The data from LAMA and WROC reference stations in Poland were used in numerical processing, using the least squares method in SciTEC software. The Differential Code Biases C1-C2 were determined based on the Geometry Free linear combination with temporal resolution of 2 hours, separately for each station. The first results of the DCB C1-C2 were compared with theoretical values based on monthly CODE’s product. Moreover, the theoretical values of SDCB C1-C2 include many anomalies and the reference sum of SDCB C1-C2 is not equal to 0. The standard deviation of the mean difference between CODE and each station is about ±3 ns. The magnitude order of SDCB C1-C2 for each station for each day is less than ±10 ns with the standard deviation less than ±0.5 ns. The average values of SDCB C1-C2 for each station were determined based on a RINEX file from 6 measurements days. The SDCB C1-C2 from each station have got similar trends, except to the bias of SVN1, where the difference is more than 2 ns. Generally, the mean difference of SDCB C1-C2 between the LAMA and WROC solution is about ±1 ns. The RDCB C1-C2 are more stable than the SDCB C1-C2, with a daily repeatability about 0.7 ns. The characteristic of RDCB C1-C2 for each station over a few days is very irregular, with the range of about ±1.5 ns

Comparison of numerical and physical models of contacts of vacuum circuit breaker

Artykuł zawiera wyniki obliczeń i pomiarów rozkładu pola magnetycznego na powierzchni nakładki styków komór wyłączników próżniowych. Obliczenia pola magnetycznego przeprowadzono za pomocą programu Maxwell 3D. Pomiary rozkładu pola wykonano za pomocą układu trzech sond mierzących pole magnetyczne (promieniowe, obwodowe, osiowe) na powierzchni styku i przeliczono dla prądu 50 kA.The paper presents results of measurements and calculations of the magnetic field distribution on models of coil contacts of HV interrupter vacuum chambers. Calculation was made using Maxwell 3D computer software. The magnetic field was measured using a system of 3 coils (axial, azimuthal, radial); the results were recalculated for 50 kA

Application of PPP-Kinematic method and aircraft positioning software for calculation of plane's position

W artykule zaprezentowano rezultaty zastosowania techniki GPS w transporcie lotniczym. Współrzędne statku powietrznego wyznaczono z użyciem kodowych obserwacji GPS dla metody PPP-Kinematic w programie APS. Eksperyment lotniczy z użyciem samolotu Cessna 172 przeprowadzono na lotnisku wojskowym w Dęblinie. Scharakteryzowano metodę PPP-Kinematic oraz opisano konfigurację parametrów wejściowych dla tej metody. Ponadto przedstawiono wartości takich parametrów jak: dokładność poprawki chodu zegara odbiornika, dokładność współrzędnych statku powietrznego, współczynniki DOP, wartości poziomów bezpieczeństwa HPL i VPL, wartości testu statystycznego Chi-kwadrat.Results of application of GPS technique in the air transport are presented in the paper. Aircraft’s coordinates were obtained using GPS code observations for PPP-Kinematic method in the APS software. Cessna 172 aircraft was applied in a flight test at Dęblin military airport. The paper describes the PPP-Kinematic method and configuration of input parameters for this method. In addition, the values of such parameters as: accuracy of receiver clock bias, accuracy of aircraft’s coordinates, DOP coefficients, accuracy of HPL and VPL terms, results of statistical test Chi-square are presented in the paper