4 research outputs found
Genetic dissection of the biochemical activities of human DNA repair protein, APE1
Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key DNA repair enzyme
involved in both base excision repair (BER) and nucleotide incision repair (NIR) pathways. In the BER
pathway, APE1 cleaves DNA at AP sites and 3'-blocking moieties generated by DNA glycosylases. In the
NIR pathway, APE1 incises DNA 5' to a number of oxidatively damaged bases. Here we propose to identify
and characterize critical amino acids of APE1 involved in either BER and/or NIR functions by using
the alignment of the known three-dimensional (or tertiary) structures of Xth family AP endonucleases
including the Methanothermobacter thermautotrophicus Mth212, Bacillus subtilis ExoA (1), E. coli Xth
and human APE1 proteins (2)
Genetic dissection of the biochemical activities of human DNA repair protein, APE1
Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key DNA repair enzyme
involved in both base excision repair (BER) and nucleotide incision repair (NIR) pathways. In the BER
pathway, APE1 cleaves DNA at AP sites and 3'-blocking moieties generated by DNA glycosylases. In the
NIR pathway, APE1 incises DNA 5' to a number of oxidatively damaged bases. Here we propose to identify
and characterize critical amino acids of APE1 involved in either BER and/or NIR functions by using
the alignment of the known three-dimensional (or tertiary) structures of Xth family AP endonucleases
including the Methanothermobacter thermautotrophicus Mth212, Bacillus subtilis ExoA (1), E. coli Xth
and human APE1 proteins (2)