Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of the cross: ‘Retina’ × ‘Topaz’. Twenty three of the 27 SSR markers covered 225 cM in ‘Retina’ and 371 cM in ‘Topaz’ genome. High level of colinearity (≈85%) was found between obtained map and the respective map regions of ‘Fiesta’, ‘Discovery’, ‘Ralls Janet’ and ‘Delicious’. Only single inversions of marker positions were noted, predominantly in ‘Topaz’. CAPS and SSCP/SNP markers in seven ESTs, chosen based on the metabolic pathways of ascorbic acids and sugar, were identified. Four of the CAPS markers, linked to the genes coding: UDP glucose: flavonoid 3-o-glucosyl transferase, ascorbate peroxidase, and two sugar transporters, were mapped on LG2 (GFglTra - both cultivars), LG12 (PGiso1B and PSTS - ‘Topaz’) and LG14 (PST - ‘Retina’). According to our knowledge, loci of these markers have never been identified on the apple genome map

How to search herbicide stress in plant system at molecular level?

Application of herbicides is connected with stress in plant system. Molecular signals of stress are of significant value. How to find them

Changes In The Expression Of Three Cold-Regulated Genes In ‘Elsanta’ And ‘Selvik’ Strawberry (Fragaria × Ananassa) Plants Exposed To Freezing

Cold temperatures in midwinter and late-spring frosts cause severe damages to strawberry plants cultivated in temperate climate regions. Despite the seriousness of the problem, the plant mechanism of defense against cold stress has not been fully elucidated yet, especially in its molecular aspect. The presented investigations were conducted on the cold-susceptible cultivar ‘Elsanta’ and the cold-tolerant cultivar ‘Selvik’. Expression profiles of three genes (CBF4, COR47 and F3H) were determined at three time-points: 0, 6 and 12 weeks after sub-zero treatment at -12 °C. The CBF4 gene was very strongly up-regulated in ‘Selvik’ plants and the highest value of the transcript level was detected just after the treatment (time - point 0). The F3H transcript in the treated ‘Selvik’ plants reached the level 4 times higher than in control plants in the 12th week after treatment (time-point 3). In ‘Elsanta’ plants, the CBF4 and COR47 genes were slightly up-regulated (time-point 2), while the F3H gene showed stability of expression. High positive correlation between the transcript level of COR47 and transcript level of CBF4 genes was observed for both cultivars

The History Of Genome Mapping In Fragaria Spp.

This overview summarizes the research programs devoted to mapping the genomes within Fragaria genus. A few genetic linkage maps of diploid and octoploid Fragaria species as well as impressive physical map of F. vesca were developed in the last decade and resulted in the collection of data useful for further fundamental and applied studies. The information concerning the rules for proper preparation of mapping population, the choice of markers useful for generating linkage map, the saturation of existing maps with new markers linked to economically important traits, as well as problems faced during mapping process are presented in this paper