Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments

Abstract:A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100 mg·l-1), 4% sucrose and 8 g·l-1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse